The Role of Overexpressed Apolipoprotein AV in Insulin-Resistant Hepatocytes
Open Access
- 22 April 2020
- journal article
- research article
- Published by Hindawi Limited in BioMed Research International
- Vol. 2020, 1-13
- https://doi.org/10.1155/2020/3268505
Abstract
In this paper, we sought to explore the relationship between apolipoprotein AV (APOAV) overexpression and insulin resistance in hepatocytes. The insulin-resistant HepG2 cell model was constructed, and then, APOAV-overexpressed HepG2 cells (B-M) were induced by infecting with a recombinant adenovirus vector. Microarray data were developed from B-M samples compared with negative controls (A-con), and the microarray data were analyzed by bioinformatic methods. APOAV-overexpression induced 313 upregulated genes and 563 downregulated ones in B-M sample. The differentially expressed genes (DEGs) were significantly classified in fat digestion and absorption pathway. Protein-protein interaction network was constructed, and AGTR1 (angiotensin II receptor type 1) and P2RY2 (purinergic receptor P2Y, G-protein coupled 2) were found to be the significant nodes closely related with G-protein related signaling. Additionally, overexpression of APOAV could change the expression of Glut4 and release the insulin resistance of hepatic cells. Thus, APOAV overexpression may prevent the insulin resistance in liver cells by mediating the genes such as AGTR1 and P2RY2.Funding Information
- National Natural Science Foundation of China (81500234)
This publication has 42 references indexed in Scilit:
- Bcl10 Links Saturated Fat Overnutrition with Hepatocellular NF-κB Activation and Insulin ResistanceCell Reports, 2012
- Detecting overlapping protein complexes in protein-protein interaction networksNature Methods, 2012
- Oxidative stress and diabetes: What can we learn about insulin resistance from antioxidant mutant mouse models?Free Radical Biology & Medicine, 2012
- Reciprocal Transcriptional Regulation of Metabolic and Signaling Pathways Correlates With Disease Severity in Heart FailureCirculation: Cardiovascular Genetics, 2011
- Cellular mechanism of insulin resistance in nonalcoholic fatty liver diseaseProceedings of the National Academy of Sciences of the United States of America, 2011
- The STRING database in 2011: functional interaction networks of proteins, globally integrated and scoredNucleic Acids Research, 2010
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression dataBioinformatics, 2009
- Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNature Protocols, 2008
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003
- Seven-transmembrane receptorsNature Reviews Molecular Cell Biology, 2002