Lactobacillus plantarum FRT10 alleviated high-fat diet–induced obesity in mice through regulating the PPARα signal pathway and gut microbiota
- 14 May 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Applied Microbiology and Biotechnology
- Vol. 104 (13), 5959-5972
- https://doi.org/10.1007/s00253-020-10620-0
Abstract
Previous studies showed that probiotics supplementation contributed to alleviate obesity. This work was to assess the efficacy of Lactobacillus plantarum FRT10 from sour dough in alleviating obesity in mice fed with a high-fat diet (HFD), and the underlying mechanisms focusing on modulation of the gut microbiota profile. Kunming mice were fed with a regular diet (CT), a high-fat diet (HFD), and two HFDs containing low and high doses of L. plantarum FRT10 for 8 weeks. The physiological and biochemical modulations in liver were analyzed. Cecal contents were analyzed by high-throughput 16S ribosomal RNA sequencing. FRT10 supplementation significantly reduced body weight gain, fat weight, and liver triacylglycerols (TGs) and alanine aminotransferase (ALT) concentrations (P < 0.05). FRT10 significantly ameliorated the HFD-induced gut dysbiosis, as evidenced by increased abundance of microbes, including Butyricicoccus, Butyricimonas, Intestinimonas, Odoribacter, and Alistipes, and decreased abundance of Desulfovibrionaceae, Roseburia, and Lachnoclostridium. Lactobacillus, Bifidobacterium, and Akkermansia were markedly increased after FRT10 intervention. In addition, real-time quantitative PCR revealed that FRT10 upregulated the mRNA expression levels of peroxisome proliferator–activated receptor-α (PPARα) and carnitine palmitoyltransferase-1α (CPT1α), and downregulated the mRNA expression levels of sterol regulatory element–binding protein 1 (SREBP-1) and TG-synthesizing enzyme diacylglycerol acyltransferase 1 (DGAT1) in liver. These findings suggested that FRT10 had anti-obesity effects in obese mice partly related to the activation of PPARα/CPT1α pathway. FRT10 can be considered a single probiotic agent for preventing HFD-induced obesity in humans and animals.Keywords
Funding Information
- National Basic Research Program of China (973 Program) (2017YFD0400303)
- National Natural Science Foundation of China (31802081)
- Agricultural Science and Technology Innovation Program of China (ASTIP-FRI07)
This publication has 55 references indexed in Scilit:
- SIRT3 Deficiency Promotes High‐Fat Diet‐Induced Nonalcoholic Fatty Liver Disease in Correlation with Impaired Intestinal Permeability through Gut Microbial DysbiosisMolecular Nutrition & Food Research, 2018
- Sugary Kefir Strain Lactobacillus mali APS1 Ameliorated Hepatic Steatosis by Regulation of SIRT‐1/Nrf‐2 and Gut Microbiota in RatsMolecular Nutrition & Food Research, 2018
- Short-chain fatty acids in control of body weight and insulin sensitivityNature Reviews Endocrinology, 2015
- UPARSE: highly accurate OTU sequences from microbial amplicon readsNature Methods, 2013
- Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesityProceedings of the National Academy of Sciences of the United States of America, 2013
- Activation of peroxisome proliferator-activated receptor-alpha stimulates both differentiation and fatty acid oxidation in adipocytesJournal of Lipid Research, 2011
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Interplay between obesity and associated metabolic disorders: new insights into the gut microbiotaCurrent Opinion in Pharmacology, 2009
- Obesity and nonalcoholic fatty liver disease: Biochemical, metabolic, and clinical implicationsJournal of Hepatology, 2009
- DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarumJournal of Applied Microbiology, 2006