Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader
Open Access
- 17 February 2021
- journal article
- research article
- Published by IWA Publishing in Water Science & Technology
- Vol. 83 (6), 1399-1406
- https://doi.org/10.2166/wst.2021.072
Abstract
Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel simple and high throughput method developed in our laboratory to enumerate E. coli concentrations in RW samples. The method is based on the use of the synthetic substrate specific for the β-D-glucuronidase (GUS) produced by E. coli. GUS activities and E. coli concentrations were monitored at eight selected sites in rivers running through Sapporo, Japan. Because the fluorescence intensities of the synthetic substrate in the RW samples increased linearly over a 4-h incubation period, we could estimate the GUS activities of the RW samples. The GUS activities were highly correlated with E. coli concentrations at >100 most probable numbers 100 mL−1 with a correlation coefficient of 0.87. The GUS activities of the RW samples collected from all sampling sites fitted well to a single correlation equation, which indicates that it was applicable to the estimation of E. coli concentrations regardless of the sampling sites. This method is simple, rapid, reliable, inexpensive, and high throughput, and is therefore useful for monitoring E. coli in RW.Keywords
Funding Information
- Japan Science and Technology Agency (JPMJMI18DB)
- Japan Society for the Promotion of Science (20KK0090)
- Japan Society for the Promotion of Science (19K21979)
- Japan Society for the Promotion of Science (17K18894)
- Ministry of Land, Infrastructure, Transport and Tourism (GAIA_2016-4)
- Northern Advancement Center for Science and Technology (2016-Startup-7)
- Toda Scholarship Foundation (2016-1)
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