WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age‐mediated flowering
- 12 May 2020
- journal article
- research article
- Published by Wiley in Journal of Integrative Plant Biology
- Vol. 62 (11), 1659-1673
- https://doi.org/10.1111/jipb.12946
Abstract
WRKY12 and WRKY13 are two WRKY transcription factors that play important roles in the control of flowering time under short‐day (SD) conditions. The temporally regulated expression of WRKY12 and WRKY13 indicates that they may be involved in the age‐mediated flowering pathway. However, their roles in this pathway are poorly understood. Here, we show that the transcription of WRKY12 and WRKY13 is directly regulated by SQUAMOSA PROMOTER BINDING–LIKE 10 (SPL10), a transcription factor downstream of the age pathway. Binding and activation analyses revealed that SPL10 functions as a positive regulator of WRKY12 and a negative regulator of WRKY13. Further mechanistic investigation revealed that WRKY12 and WRKY13 physically interact with SPL10 and that both of them bind to the promoter of miR172b. Thus, the WRKY12‐SPL10 and WRKY13‐SPL10 interactions facilitate and inhibit SPL10 transcriptional function, respectively, to regulate miR172b expression. Together, our results show that WRKY12 and WRKY13 participate in the control of age‐mediated flowering under SD conditions though physically interacting with SPLs and co‐regulating the target gene miR172b.Keywords
Funding Information
- National Natural Science Foundation of China (31801023, 31671274)
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
This publication has 47 references indexed in Scilit:
- Arabidopsis VQ10 interacts with WRKY8 to modulate basal defense against Botrytis cinereaJournal of Integrative Plant Biology, 2018
- Arabidopsis WRKY45 Interacts with the DELLA Protein RGL1 to Positively Regulate Age-Triggered Leaf SenescenceMolecular Plant, 2017
- WRKY8 transcription factor functions in the TMV-cg defense response by mediating both abscisic acid and ethylene signaling in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2013
- SnapShot: Control of Flowering in ArabidopsisCell, 2010
- Seasonal and developmental timing of floweringThe Plant Journal, 2010
- Integrating hormones into the floral‐transition pathway of Arabidopsis thalianaPlant, Cell & Environment, 2009
- MicroRNAs: Target Recognition and Regulatory FunctionsCell, 2009
- Evolution of plant microRNAs and their targetsTrends in Plant Science, 2008
- The WRKY superfamily of plant transcription factorsTrends in Plant Science, 2000
- Functional analysis of the Arabidopsis thaliana SBP‐box gene SPL3: a novel gene involved in the floral transitionThe Plant Journal, 1997