MiR-142-3p ameliorates high glucose-induced renal tubular epithelial cell injury by targeting BOD1
- 18 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Clinical and Experimental Nephrology
- Vol. 25 (11), 1182-1192
- https://doi.org/10.1007/s10157-021-02102-y
Abstract
Background Tubular injury plays a crucial role in the pathogenesis of diabetic nephropathy (DN). It is well known that many microRNAs (miRNAs) exert crucial effects on tubular injury. This study intends to explore the effect of miR-142-3p on the apoptosis and oxidative stress of high glucose (HG)-treated renal tubular epithelial cells (HK-2) and its underlying mechanism. Materials and methods HK-2 cells were exposed to HG to mimic cell injury. MTT assays and flow cytometry analyses were conducted to measure cell viability and cell apoptosis, respectively. RT-qPCR and western blot analyses were carried out to detect RNA and protein levels, respectively. The levels of oxidative stress markers were evaluated by ELISA. The binding between miR-142-3p and biorientation of chromosomes in cell division 1 (BOD1) was validated by a luciferase reporter assay. Result MiR-142-3p is low-expressed in HG-stimulated HK-2 cells. Functionally, miR-142-3p overexpression attenuates the apoptosis and oxidative stress of HG-stimulated HK-2 cells. Mechanistically, BOD1 was confirmed to be targeted by miR-142-3p in HK-2 cells. Moreover, BOD1 overexpression reversed the suppressive effect of miR-142-3p overexpression on the apoptosis and oxidative stress of HK-2 cells treated with HG. Conclusion MiR-142-3p ameliorates HG-induced renal tubular epithelial cell injury by targeting BOD1. The finding might provide novel insight into the role of miR-142-3p/BOD1 axis in DN treatment.Keywords
Funding Information
- Henan science and technology research project (162102310287)
- Henan health and Family Planning Commission provincial Ministry co construction project (201701022)
- Henan Medical Science and technology research project (2018020395)
This publication has 56 references indexed in Scilit:
- Study of microRNA in diabetic nephropathy: Isolation, quantification and biological functionNephrology, 2015
- Identification of microRNA biomarkers in type 2 diabetes: a meta-analysis of controlled profiling studiesDiabetologia, 2015
- Overview of MicroRNA BiologySeminars in Liver Disease, 2015
- Bod1 regulates protein phosphatase 2A at mitotic kinetochoresNature Communications, 2013
- MicroRNAs: a new ray of hope for diabetes mellitusProtein & Cell, 2012
- miRmap: Comprehensive prediction of microRNA target repression strengthNucleic Acids Research, 2012
- Autophagy Protects the Proximal Tubule from Degeneration and Acute Ischemic InjuryJournal of the American Society of Nephrology, 2011
- The role of site accessibility in microRNA target recognitionNature Genetics, 2007
- A Pattern-Based Method for the Identification of MicroRNA Binding Sites and Their Corresponding HeteroduplexesCell, 2006
- Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA TargetsCell, 2005