Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS
Open Access
- 3 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Genome Biology
- Vol. 21 (1), 1-9
- https://doi.org/10.1186/s13059-020-01969-6
Abstract
We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.Funding Information
- Cancer Research UK (C63763/A26394, C63763/A27122)
- Innovation Programme Consortia HEP-CAR (667273)
- Medical Research Council (MR/R022011/1)
- Wellcome Trust (IA 200838/Z/16/Z)
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