Advantage of fat-derived CD73 positive cells from multiple human tissues, prospective isolated mesenchymal stromal cells
Open Access
- 15 September 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 10 (1), 1-9
- https://doi.org/10.1038/s41598-020-72012-8
Abstract
Somatic stem cells have been isolated from multiple human tissues for their potential usefulness in cell therapy. Currently, mesenchymal stromal cells (MSCs) are prepared after several passages requiring a few months of cell culture. In this study, we used a prospective isolation method of somatic stem cells from gestational or fat tissues, which were identified using CD73 antibody. CD73-positive population from various tissues existed individually in flowcytometric pattern, especially subcutaneous fat- and amniotic-derived cells showed the highest enrichment of CD73-positive cells. Moreover, the cell populations isolated with the prospective method showed higher proliferative capacity and stem cell marker expression, compared to the cell populations which isolated through several passages of culturing whole living cells: which we named “conventional method” in this paper. Furthermore, the therapeutic potential of CD73-positive cells was evaluated in vivo using a mouse model of pulmonary fibrosis. After intranasal administration, murine CD73-positive cells reduced macrophage infiltration and inhibited fibrosis development. These results suggest that further testing using CD73-positive cells may be beneficial to help establish the place in regenerative medicine use.Funding Information
- Japan Agency for Medical Research and Development (Practical Applications of Regenerative Medicine, Practical Applications of Regenerative Medicine)
- Uehara Memorial Foundation
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Science and Technology Agency (JPMJMI18CB, JPMJMI18CB, JPMJMI18CB)
- Takeda Science Foundation
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