Cyclo olefin polymer-based solvent-free mass-productive microphysiological systems
- 1 March 2021
- journal article
- research article
- Published by IOP Publishing in Biomedical Materials
- Vol. 16 (3), 035009
- https://doi.org/10.1088/1748-605x/abe660
Abstract
A microphysiological system (MPS) holds great promise for drug screening and toxicological testing as an alternative to animal models. However, this platform faces several challenges in terms of the materials used (e.g. polydimethylsiloxane; PDMS). For instance, absorption of drug candidates and fluorescent dyes into PDMS, as well as the effect elicited by materials on cultured cells, can cause inaccurate or misleading results in cell assays. The use of PDMS also poses challenges for mass production and long-term storage of fabricated MPSs. Hence, to circumvent these issues, herein we describe the development of a cyclo olefin polymer (COP)-based MPS using photobonding processes and vacuum ultraviolet (VUV), designated as COP-VUV-MPS. COP is an amorphous polymer with chemical/physical stability, high purity and optical clarity. Due to the thermostability and high modulus of COP, the metal molding processes was applied for mass production of MPSs without deformation of microstructures and with quick fabrication cycle time (approx. 10 min/cycle). Moreover, VUV photobonding process with an excimer light at a 172nm wavelength allowed assembling COP materials without the use of additional solvents and tapes, which might cause cell damages. In comparison with the conventional MPS made of PDMS (PDMS-MPS), COP-VUV-MPS showed improved chemical resistance without causing molecule absorption. Moreover, COP-VUV-MPS maintained the stemness of environmentally sensitive human-induced pluripotent stem cells without causing undesired cellular phenotypes or gene expression. These results suggest that COP-VUV-MPS may be broadly applicable for the advancement of MPS and applications in drug development, as well as in vitro toxicological testing.Keywords
Funding Information
- Japan Society for the Promotion of Science (16K14660)
This publication has 40 references indexed in Scilit:
- Chemically defined conditions for human iPSC derivation and cultureNature Methods, 2011
- Cyclic olefin polymers: emerging materials for lab-on-a-chip applicationsMicrofluidics and Nanofluidics, 2010
- Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditionsLab on a Chip, 2010
- Microfluidic chip made of COP (cyclo-olefin polymer) and comparion to PMMA (polymethylmethacrylate) microfluidic chipJournal of the American Academy of Dermatology, 2008
- Induced Pluripotent Stem Cell Lines Derived from Human Somatic CellsScience, 2007
- Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined FactorsCell, 2007
- A ROCK inhibitor permits survival of dissociated human embryonic stem cellsNature Biotechnology, 2007
- Telomere and telomerase in stem cellsBritish Journal of Cancer, 2007
- Nanog and transcriptional networks in embryonic stem cell pluripotencyCell Research, 2007
- Embryonic Stem Cell Lines Derived from Human BlastocystsScience, 1998