Application of antimicrobial peptide Mytichitin‐A in pork preservation during refrigerated storage
- 20 February 2021
- journal article
- research article
- Published by Hindawi Limited in Journal of Food Processing and Preservation
- Vol. 45 (5), e15404
- https://doi.org/10.1111/jfpp.15404
Abstract
Antimicrobial peptide Mytichitin‐A has been expressed in large quantities in Pichia pastoris and has good antibacterial activity against Gram‐positive bacteria. To explore its potential as a food preservative in a pork system, the activity of P. pastoris‐derived Mytichitin‐A against Pseudomonas spp., one of dominant spoilage flora in meat products, was first conducted. Results showed that Mytichitin‐A effectively inhibited the growth of both P. fluorescens and P. tolaasii. Moreover, it exhibited a broad range of pH and thermal stability, was resistant to various protease digestion and amino acid interferences, and had very low hemolytic activity. Importantly, 25 μg/ml Mytichitin‐A could effectively inhibit the bacterial growth, the increase of drip loss, pH value, lipid oxidation, and total volatile alkaline nitrogen during pork storage. Thus, it was conducive to the maintenance of pork quality. These results suggested that P. pastoris‐derived Mytichitin‐A has excellent potential as a preservative for fresh pork. Practical applications This research reveals that Mytichitin‐A has a great potential for the fresh pork preservation during storage and could conducive to the maintenance of pork quality. The completion of this study also contributes to the development of novel preservation method to maintain meat quality in the future.Keywords
Funding Information
- Natural Science Foundation of Tianjin City (18JCZDJC34100)
- Beijing Technology and Business University
This publication has 44 references indexed in Scilit:
- A Novel Antimicrobial Peptide from Skin Secretions of the Tree FrogTheloderma kwangsiensisZoological Science, 2013
- The Future of Pork Production in the World: Towards Sustainable, Welfare-Positive SystemsAnimals, 2013
- Sensory characteristics of spoilage and volatile compounds associated with bacteria isolated from cooked and peeled tropical shrimps using SPME–GC–MS analysisInternational Journal of Food Microbiology, 2011
- Oxidative Stability of Pork Longissimus Muscle Injected with Marbling-Like Emulsified LipidsAdvanced Materials Research, 2011
- Emerging carbapenemases: a global perspectiveInternational Journal of Antimicrobial Agents, 2010
- Expression of antimicrobial peptides in cutaneous infections after skin surgeryBritish Journal of Dermatology, 2010
- Pentocin 31-1, a novel meat-borne bacteriocin and its application as biopreservative in chill-stored tray-packaged pork meatFood Control, 2010
- A review of bacteriocinogenic lactic acid bacteria used as bioprotective cultures in fresh meat produced in ArgentinaMeat Science, 2008
- Meat spoilage during distributionMeat Science, 2008
- Benchmarking value in the pork supply chain: Characterization of US pork in the retail marketplaceMeat Science, 2005