First Investigative Study of Azole-Resistant Aspergillus fumigatus in the Environment in Burkina Faso
Open Access
- 25 February 2021
- journal article
- research article
- Published by MDPI AG in International Journal of Environmental Research and Public Health
- Vol. 18 (5), 2250
- https://doi.org/10.3390/ijerph18052250
Abstract
Azole-resistant Aspergillus fumigatus (ARAF) strains have been reported on all continents, however, limited data exist on these strains in Africa, while several factors, mainly environmental ones, suggest their presence on this continent. This study aimed to assess the environmental prevalence of ARAF strains in Burkina Faso, a country situated in the West African region where data on ARAF is non-existent. In total, 120 environmental samples (soil) were collected and analyzed. Samples were screened for resistance using three azole-containing agar plates; one without azole antifungal (growth control) and two supplemented with either itraconazole (4 mg/L) or voriconazole (2 mg/L). The EUCAST susceptibility testing method was used to confirm the azole-resistant phenotype of A. fumigatus sensu-stricto isolates. Mutations in the cyp51A gene were determined by sequencing. Of the 120 samples, 51 positive samples showed growth of A. fumigatus isolates on control medium. One ARAF (2%; 1/51) isolate was found amongst A. fumigatus positive samples and harbored the F46Y/M172V/E427K cyp51A mutations. No TR34/L98H or TR46/Y121F/T289A mutations were observed. Our study described the first A. fumigatus isolate resistant to an azole antifungal in Burkina Faso.Funding Information
- Académie de recherche et d'enseignement supérieur (PFS - Master professionnel en mycologie médicale)
This publication has 22 references indexed in Scilit:
- Multi-centric evaluation of the online MSI platform for the identification of cryptic and rare species of Aspergillus by MALDI-TOFMedical Mycology, 2019
- Triazole resistance in Aspergillus fumigatus: recent insights and challenges for patient managementClinical Microbiology & Infection, 2018
- Triazole-Resistant Aspergillus fumigatus from Fungicide-Experienced Soils in Naivasha Subcounty and Nairobi County, KenyaInternational Journal of Microbiology, 2018
- Triazole resistance surveillance in Aspergillus fumigatusMedical Mycology, 2018
- Aspergillus fumigatus carrying TR34/L98H resistance allele causing complicated suppurative otitis media in Tanzania: Call for improved diagnosis of fungi in sub-Saharan AfricaBMC Infectious Diseases, 2016
- Use of Chemical Pesticides in Ethiopia: A Cross-Sectional Comparative Study on Knowledge, Attitude and Practice of Farmers and Farm Workers in Three Farming SystemsAnnals of Occupational Hygiene, 2016
- Nationwide Surveillance of Azole Resistance in Aspergillus DiseasesAntimicrobial Agents and Chemotherapy, 2015
- Multi-azole-resistant Aspergillus fumigatus in the environment in TanzaniaJournal of Antimicrobial Chemotherapy, 2014
- Hidden Killers: Human Fungal InfectionsScience Translational Medicine, 2012
- Triazole Fungicides Can Induce Cross-Resistance to Medical Triazoles in Aspergillus fumigatusPLOS ONE, 2012