Escherichia coli Strains with Virulent Factors Typical for Uropathogens were Isolated from Sinuses from Patients with Chronic Rhinosinusitis—Case Report
Open Access
- 24 April 2020
- Vol. 9 (5), 318
- https://doi.org/10.3390/pathogens9050318
Abstract
Escherichia coli were isolated from three patients with chronic rhinosinusitis (CRS) by intraoperative sinus tissue biopsy. Taking into account the unusual replicative niche and previous treatment failures, it was decided to focus on the virulence and drug resistance of these bacteria. The strains turned out to be multi-sensitive, but the rich virulence factors profile of bacteria typical for phylogenetic group B2 deserved attention. Tests were carried out for the presence of 32 genes using the PCR method. Particularly noteworthy are the toxins Cnf-1, HlyA, Usp—an extensive iron uptake system (enterobactin, salmochelin, yersiniabactin and outer membrane hemin receptor ChuA)—SPATE autotransporters such as vat and pic, Ag43 autoaggregative protein—important for biofilm formation—and TosA/B which enhance the fitness of E.coli. All these virulence factors are identified predominantly in UPEC strains and provide a fitness advantage during colonization of the sinuses. Patients with CRS should be asked for past or present UTI. The specific virulence factors of E. coli that facilitate the colonization of the GI tract and urinary tract may also favor the colonization of a new ecological niche (sinuses) as a result of microbial imbalance or dysbiosis.Keywords
This publication has 23 references indexed in Scilit:
- A subset of two adherence systems, acute pro-inflammatory pap genes and invasion coding dra, fim, or sfa, increases the risk of Escherichia coli translocation to the bloodstreamEuropean Journal of Clinical Microbiology & Infectious Diseases, 2013
- The Clermont Escherichia coli phylo‐typing method revisited: improvement of specificity and detection of new phylo‐groupsEnvironmental Microbiology Reports, 2012
- Presence of Putative Repeat-in-Toxin Gene tosA in Escherichia coli Predicts Successful Colonization of the Urinary TractmBio, 2011
- Involvement of IbeA in Meningitic Escherichia coli K1‐Induced Polymorphonuclear Leukocyte Transmigration Across Brain Endothelial CellsBrain Pathology, 2010
- (CGG) 4 -Based PCR as a Novel Tool for Discrimination of Uropathogenic Escherichia coli Strains: Comparison with Enterobacterial Repetitive Intergenic Consensus-PCRJournal of Clinical Microbiology, 2009
- Genomic Islands of Uropathogenic Escherichia coli Contribute to VirulenceJournal of Bacteriology, 2009
- Identification of Type 3 Fimbriae in UropathogenicEscherichia coliReveals a Role in Biofilm FormationJournal of Bacteriology, 2008
- High prevalence of Escherichia coli belonging to the B2+D phylogenetic group in inflammatory bowel diseaseGut, 2007
- PicU, a second serine protease autotransporter of uropathogenic Escherichia coliFEMS Microbiology Letters, 2004
- Bacteriologic Findings Associated with Chronic Bacterial Maxillary Sinusitis in AdultsClinical Infectious Diseases, 2002