Journal of Analytical Methods in Chemistry
ISSN / EISSN: 20908865 / 20908873
Published by: Hindawi Limited
Total articles ≅ 1,356
Latest articles in this journal
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-19; https://doi.org/10.1155/2022/1748324
Yinqiao powder, with significant antiinflammatory and antiviral effects, is a classical formula for the treatment of febrile diseases in China. During the SARS period in 2003, Yinqiao powder showed a good antipyretic effect. It also plays a major role in the treatment for COVID-19 in China. Although there are many studies on the chemical compositions and pharmacological effects of Yinqiao powder, there are few studies on the quality standard system of it. In our study, a systematic quality evaluation method of Yinqiao powder combining HPLC fingerprint with quantitative analysis of multi-components by single marker (QAMS) based on network pharmacology and UPLC-Q-Exactive-Orbitrap-MS was established for the first time. In the UPLC-Q-Exactive-Orbitrap-MS experiment, a total of 53 compounds were identified in the extract solution of Yinqiao powder. In addition, 33 blood components were characterized, 23 of which were prototypes. The results of network pharmacology analysis showed that Yinqiao powder may inhibit inflammatory responses by suppressing IL-6, CXCL2, TNFα, NF-κB, etc., in the treatment of COVID-19. The HPLC fingerprint analysis of Yinqiao powder was conducted at 237nm and 29 characteristic peaks were matched, 11 of which were identified. Forsythoside A was selected as the internal standard reference and double-wavelength (237nm and 327nm) was established in QAMS experiment. The repeatability was well under different conditions, and the results measured by QAMS were consisted with that of the external standard method (ESM), indicating that the QAMS method was reliable and accurate. The quality evaluation method of Yinqiao powder would be helpful to evaluate the intrinsic quality of Yinqiao powder more comprehensively, which is conducive to improve the quality standard of Yinqiao powder and provide a beneficial guarantee for the clinical treatment of COVID-19.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-11; https://doi.org/10.1155/2022/8026410
Dendrobium officinale (D. officinale) is a valuable traditional Chinese herbal medicine with high commercial value. In Chinese Pharmacopoeia (Ch.P., 2020 edition), the quality of D. officinale is mainly evaluated by its polysaccharide content. However, varying growth and production conditions, such as cultivation environment, origin, harvesting process, or processing methods, resulting in highly variable yields, quality, and composition. The aim of this study was to investigate whether the content of secondary metabolites in D. officinale from different origins is consistent with the polysaccharide content. The results showed that the polysaccharide content and pass rate were ranked as GX > AH > GZ > YN. Based on the nontargeted metabolomics approach, we searched for differential components in 22 different regions of D. officinale, including amides, bibenzyls, disaccharide, flavonoids, organic nitrogenous compounds, and phenolic glycosides. The overall expression was opposite to the polysaccharide, and the most expressed was YN, followed by GZ, AH, and GX. These results indicated that the current quality standard for evaluating the quality of D. officinale by polysaccharide content alone is imperfect, and small molecule compounds need to be included as quality markers.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-12; https://doi.org/10.1155/2022/4610140
Proline is an important amino acid that widely affects life activities. It plays an important role in the occurrence and development of diseases. It is of great significance to monitor the metabolism of the machine. With the great advantages of deep learning in feature extraction, near-infrared analysis technology has great potential and has been widely used in various fields. This study explored the potential application of near-infrared spectroscopy in the detection of serum proline. We collected blood samples from clinical sources, separated the serum, established a quantitative model, and determined the changes in proline. Four algorithms of SMLR, PLS, iPLS, and SA were used to model proline in serum. The root mean square errors of prediction were 0.00111, 0.00150, 0.000770, and 0.000449, and the correlation coefficients (Rp) were 0.84, 0.67, 0.91, and 0.97, respectively. The experimental results show that the model is relatively robust and has certain guiding significance for the clinical monitoring of proline. This method is expected to replace the current mainstream but time-consuming HPLC, or it can be applied to rapid online monitoring at the bedside.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-12; https://doi.org/10.1155/2022/6575140
In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10fM to 1μM with a detection limit of 7.96fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-12; https://doi.org/10.1155/2022/5822562
Pacific abalone (Haliotis discus hannai) is a commercially important mollusk; therefore, improvement of its growth performance and quality has been emphasized. During embryonic development, abalones undergo a series of distinct larval stages, including swimming veliger larvae, juveniles, and mature individuals, and their biomolecular composition varies depending on the developmental stage. Therefore, in the present study, we performed untargeted lipid profiling of abalone tissues at different developmental stages as well as the hemolymph of mature female and male abalones using ultrahigh-performance liquid chromatography-tandem mass spectrometry. These profiles can provide meaningful information to understand compositional changes in lipids through abalone metamorphosis and development. A total of 132 lipids belonging to 15 classes were identified from abalone tissues at different developmental stages. Moreover, 21 lipids belonging to 8 classes were identified from the hemolymph of mature abalones. All data were processed following strict criteria to provide accurate information. Triglycerides and phosphatidylcholines were the major lipid components identified in both tissues and hemolymph, accounting for, respectively, 27 and 15 of all lipids in tissues and, respectively, 24 and 38 of all lipids in the hemolymph. Of note, lysophosphatidylcholine was only detected in the tissues of mature abalones, paving the way for further analyses of abalone lipids based on developmental stages. The present findings offer novel insights into the lipidome of abalone tissues and hemolymph at different developmental stages, building a foundation for improving the efficiency and quality of abalone aquaculture.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-11; https://doi.org/10.1155/2022/2915018
With the continuous development of medical science and technology, especially with the advent of the era of precision diagnosis and treatment, molecular biology detection technology is widely valued and applied as an aid to early diagnosis of tuberculosis. The GeneXpert Mycobacterium tuberculosis Branching (MTB) technology is a suite of semi-nested real-time fluorescent quantitative PCR in vitro diagnostic technologies developed by Cepheid Inc. It targets the rifampicin resistance gene, rpoB, and can detect both MTB and resistance to rifampicin within 2h. This review analyzed the papers related to GeneXpert using bibliometric software CiteSpace and Bibliometrix. A total of 151 articles were analyzed, spanning from 2011 to 2021. This bibliometrics-based review summarizes the history of the development of GeneXpert in tuberculosis diagnosis and its current status. Contributions of different countries to the topic, journal analysis, key paper analysis, and clustering of keywords were used to analyze this topic.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-10; https://doi.org/10.1155/2022/2080600
The determination of trace metals in seawater is an important project of marine environmental monitoring. However, the presence of many alkali metal ions with high concentration, such as sodium ion, seriously interferes with the detection limit and accuracy of atomic absorption spectrometry (AAS, flame/graphite furnace integrated). The conventional chemical methods for the enrichment of trace metals are complex, and low boiling point organic solvents are used. In this paper, a kind of commercial cross-linked polystyrene resin microspheres was chloromethylated and aminated to introduce EDTA-type amino polycarboxylic groups and then loaded in a column as the absorption filler. A set of seawater pretreatment and enrichment devices was designed and assembled. The enriching process and conditions of trace Cu, Zn, Pb, and Cd in standard seawater were studied. 10 g of the modified resin could enrich the equivalent seawater and remove successfully the light metal ions. pH = 5∼9 and 0.2 mL/min of the flow rate were the suitable conditions for preconcentration. The enriched metal ions in the eluent were analyzed on the AAS. Compared with the conventional solvent method, the novel material and enrichment device have high preconcentration efficiency, strong anti-interference ability, and low cost and could be directly applied for routine seawater detection.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-7; https://doi.org/10.1155/2022/2819855
This paper describes a capillary electrophoresis method for the determination of the cationic B-vitamins thiamine, nicotinamide, pyridoxine, pyridoxal, and pyridoxamine in untreated cell culture medium samples. The effects of the buffering capacity, the mobility of the coion, and the preconditioning solution on the robustness of the method were investigated. Using a 100mM phosphoric acid and 55mM triethanolamine background electrolyte at pH 2.3 and capillary preconditioning with 1M NaOH, all five vitamins could be separated with good resolution. Preliminary method validation data over the range 10110µM for undiluted samples, with 10μM being the lower range limit of quantification QL, showed accuracy recoveries of 94104, and migration time and peak area repeatabilities within 0.4 RSD and 2.6 RSD, respectively.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-9; https://doi.org/10.1155/2022/5607347
Diosgenin, a steroidal sapogenin, has attracted attention worldwide owing to its pharmacological properties, including antitumor, cardiovascular protective, hypolipidemic, and anti-inflammatory effects. The current diosgenin analysis methods have the disadvantages of long analysis time and low sensitivity. The aim of the present study was to establish an efficient, sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach for pharmacokinetic analysis of diosgenin amorphous solid dispersion (ASD) using tanshinone IIA as an internal standard (IS). Male Sprague-Dawley rats were orally administered diosgenin ASD, and orbital blood samples were collected for analysis. Protein precipitation was performed with methanol-acetonitrile (50:50, v/v), and the analytes were separated under isocratic elution by applying acetonitrile and 0.03 formic acid aqueous solution at a ratio of 80:20 as the mobile phase. MS with positive electron spray ionization in multiple reaction monitoring modes was applied to determine diosgenin and IS with m/z 415.2271.2 and m/z 295.2277.1, respectively. This approach showed a low limit of quantification of 0.5ng/ml for diosgenin and could detect this molecule at a concentration range of 0.5 to 1,500ng/ml (r=0.99725). The approach was found to have intra- and inter-day precision values ranging from 1.42 to 6.91 and from 1.25 to 3.68, respectively. Additionally, the method showed an accuracy of -6.54 to 4.71. The recoveries of diosgenin and tanshinone IIA were 85.81100.27 and 98.29, respectively, with negligible matrix effects. Diosgenin and IS were stable under multiple storage conditions. Pharmacokinetic analysis showed that the Cmax and AUC0⟶t of diosgenin ASD were significantly higher than those of the bulk drug. A sensitive, simple, UPLC-MS/MS analysis approach was established and used for the pharmacokinetic analysis of diosgenin ASD in rats after oral administration.
Journal of Analytical Methods in Chemistry, Volume 2022, pp 1-9; https://doi.org/10.1155/2022/6788394
To study the differences in phenolic compounds between tobacco smokers saliva and mainstream smoke, a method was developed for the analysis of 12 phenolic compounds in saliva and mainstream smoke based on ultrahigh-performance liquid chromatography with fluorescence detection (UPLC-FLD). The contents and distributions of phenolic compounds in tobacco smokers saliva and mainstream smoke were compared. The results were as follows: (1) Phenolic compounds were quantitatively analyzed by the internal standard method using 4-fluorophenol as an internal standard. For smokers saliva samples, the limits of quantification (LOQs) ranged from 2.2 to 19.1μg/L, and the recoveries were from 80.2 to 119.2 at the three spiked levels. For mainstream smoke samples, the LOQs ranged from 0.03 to 0.26μg/cig, and the recoveries ranged from 84.9 to 107.0 at the three spiked levels. (2) The contents of phenolic compounds from 14 cigarettes in mainstream smoke and smokers saliva were determined. In mainstream smoking, the main phenolic compounds were hydroquinone, catechol, phenol, meta- and para-Cresol, and o-methylhydroquinone. In smokers saliva, the main phenolic compounds were phenol and meta- and para-Cresol and the contents of phenolic compounds in smokers saliva from different cigarettes were significantly different. (3) The content distribution patterns of phenolic compounds in smokers saliva differed from those in mainstream smoke. The predominant phenolic compound in mainstream smoke was dihydroxybenzene, while monophenols predominated in smokers saliva. (4) The contents of phenolic compounds from five kinds of cigarettes were analyzed in the saliva of different smokers using principal component analysis, which indicated that cigarettes with different sensory effects were clearly distinguished by differences in the contents of phenolic compounds in saliva.