Bioscience Biotechnology Research Communications

Journal Information
ISSN / EISSN: 09746455 / 23214007
Total articles ≅ 2,125

Latest articles in this journal

Bioscience Biotechnology Research Communications, Volume 15, pp 466-474; https://doi.org/10.21786/bbrc/15.3.14

Abstract:
The study was conducted to examine the effect of plant growth promoting rhizobacteria; Pseudomonas punonensis LMT03 (R1), Pseudomonas plecoglossicida (R4), Pseudomonas aeruginosa DSM 50071 (R2), Alcaligenes faecalis (DBHU5) and their consortium on yield and lignin deposition of the barley crop. Consortium treated plants had the highest plant height, leaf surface area, number of fertile tillers, spike length, grains per spike, 1000 grain weight, grain yield, straw yield, total biomass, and harvest index percent and the lowest values were found in control plots. The consortium treated plant produced the highest grain yield 7976 kg/ha, while control plants produced 3200 kg/ha. In comparison to the control plant, the PGPR-treated barley plant showed dense lignin deposition in the vascular bundles of the stem section.This is the first report on the effect of P.punonensis and P. plecoglossicida on barley crop yield parameters under field conditions, also the first report on lignin deposition in barley plant treated with P. punonensis and P. plecoglossicida strains.
Sharique A Ali
Bioscience Biotechnology Research Communications, Volume 15, pp 475-479; https://doi.org/10.21786/bbrc/15.3.15

Abstract:
Plankton are small microscopic organisms, comprising diatoms and algae, which form the basis of primary production, whereas zooplankton are mainly crustaceans, rotifers and larval stages of larger animals which constitute the higher aquatic food web. Both are bio-indicators of a water body, constituting the main food for the carnivorous and omnivorous fishes in the aquatic ecosystem. In the present work, a detailed analysis of zooplankton diversity along with variations in species, and seasonal abundance with average and total population density was carried out in different types of domestic sewage oxidation ponds. Similar analysis for a comparison was done in a freshwater control pond situated in Bhopal India. In this study out of 8 ponds, 4 were selected for zooplankton analysis two being primary and two being secondary sewage oxidation ponds and one freshwater control pond was selected for comparative analysis. The results indicated that the number of zooplankton in all ponds ranged from 162 to 14776 org/L, seasonally it was observed that the sewage oxidation ponds had the maximum population of zooplankton in winter, followed by rainy and summer seasons. Regarding the population of zooplankton in the individual oxidation ponds, it was found that sewage secondary pond IIIB had the maximum zooplankton followed by IIIA. Interesting observations on the cascading effects of physicochemical parameters on plankton dynamics, including the variations in the diel population of various species of zooplankton have been recorded. The phenomenon of plankton grazing in different types of domestic sewage ponds is being reported for the first time.
Ricardo Osés Rodríguez
Bioscience Biotechnology Research Communications, Volume 15, pp 390-398; https://doi.org/10.21786/bbrc/15.3.4

Abstract:
In this work, 8 meteorological variables were modeled in the Yabú station, Cuba, for which the daily database of this meteorological station was used, where the meteorological variables were taken into account are: extreme temperatures, extreme humidity and its average value, precipitation, wind force and cloudiness corresponding to the period 1977 to 2021. A linear mathematical model was obtained using the Objective Regressive Regression (ORR) methodology for each variable, which explains its behavior according to these variables, 15, 13, 10 and 8 years in advance. The calculation of the mean error with respect to the persistence forecast in temperatures, wind strength and cloudiness, as well as the persistence model was better with respect to humidity, this allows having valuable long-term information of the weather in a locality, which results in better decision making in the different aspects of the economy and society that are impacted by the weather forecast. It is concluded that these models allow long-term weather forecasting, opening a new possibility for forecasting, so that weather chaos can be overcome if this way of forecasting is used; moreover, it is the first time that an ORR model is applied to weather forecasting processes for a specific day so many years in advance.
Sujata S
Bioscience Biotechnology Research Communications, Volume 15, pp 399-406; https://doi.org/10.21786/bbrc/15.3.5

Abstract:
Keratinases from Aneurinibacillus aneurinilyticus are capable of degrading keratinous proteins. Salt precipitation and diethylaminoethyly determined the purification and characterization of the enzyme. Ion-exchange and gel permeation chromatography, and SDS-PAGE. Physicochemical factors like pH, temperature, metal ions, enzyme inhibitors and substrate. To study Km and Vmax various concentrations of keratin were used for the activity of enzyme. Gel permeation chromatography with 20.84-fold purification. 203.87 U/mg specific activity showed 34KDa between 14 to 31KDa in SDS-PAGE. The number was stable at pH 7.0-9.0 400-500C, and optimum at pH 9.0 and 500C. Further stimulated by Mg2+, Ca2+, K+, Fe2+, Zn2+, Mn2+, and Na2+ inhibited by Cu2+, Co2+ and Hg2+. Ethylene diamine tetra acetic acid with the highest stimulatory effect was inhibited by Di-isopropyl fluoro phosphatase and phenyl methyl sulfonyl fluoride. Enzyme was stable with Tween-60, TritonX-100 and TritonX-114 declined with ß-mercaptoethanol. It hydrolyzed several keratinous substrates as keratin and casein were 100 and 85.47% utilized with Km=3mM, Vmax =249µmol/ml/min. Xerophytic endophytes are treasure houses as they tolerate biotic and abiotic stress, are stable at high temperatures and pH are selected, such keratinases can be used in leather processing and detergent industries.
Shikha Yadav
Bioscience Biotechnology Research Communications, Volume 15, pp 379-385; https://doi.org/10.21786/bbrc/15.3.2

Abstract:
In recent times, when the COVID pandemic has hit the world badly, there has been a marked shortage of food, water, and other essentialities, an increase in food pricing which together with other socioeconomic impacts have eventually posed threats to agriculture, food supplies, and nutritional security all over the world. Researchers worldwide are looking for potential solutions to combat poverty and hunger issues. This review focuses on the various medicinal properties of Kodo, its uses in various fields, and prospects where it could be utilized thereafter. The findings of the present rerview revealed that the Kodo millets, Paspalum scrobiculatum: are nutritionally dense when compared to the number of grains consumed. They have a greater mineral content when compared to rice and wheat. Some of them weigh fifty times as much as rice. Finger millet contains thirty times the calcium content of rice, whereas every other millet has at least half the calcium content.
Bioscience Biotechnology Research Communications, Volume 15, pp 424-430; https://doi.org/10.21786/bbrc/15.3.8

Abstract:
The goal of this investigation was to see if it was possible to produce α-amylase from agricultural waste (groundnut shell). The strain (M1) identified as Aspergillus sp. exhibited the largest clearance zone (1.6 cm) and was used in fermentation studies. The activity of α-amylase increased after 24 hours of fermentation, peaked at 72.3 U/mL on day 5, and then began to decline. The effect of optimized environmental conditions studied using OFAT, and it was discovered that pH 6, inoculum size of 1 × 107 spores/mL, incubation period of 120 h, substrate concentration of 3 percent (w/v), and temperature of 35oC were the best for producing α-amylase from groundnut shell using Aspergillus sp. In a single fermentation, these optimum conditions were used, and the experiment yielded an optimum enzyme yield of 121.3 U/mL. This research shows that groundnut shell, a low-cost and commonly available waste, could be an ideal substrate for the manufacture of value-added products.
Sharique A. Ali
Bioscience Biotechnology Research Communications, Volume 15, pp 386-389; https://doi.org/10.21786/bbrc/15.3.3

Abstract:
Reclamation or recycling wastewater is an alternative to the gradual degradation of natural water resources. Reused or domestic sewage is highly loaded with nutrients, suspended solids, organic and inorganic matter, and microorganisms that provide natural food for several species of edible fishes. The effluent contains excessive nutrients which may increase the growth of aquatic plants and stimulate the production of natural food for fish. Oxidation ponds or stabilization ponds in the tropics are recognized as effective and economical units for the treatment of domestic sewage as well as biodegradable industrial wastes if managed properly. The driving force in a waste oxidation pond is solar energy utilized by active continuous photosynthesis. The action of sunlight on algae in the pond enables them to grow and rapidly consume the nutrients contained in the sewage. The algae and bacteria play an inter-dependent symbiotic role in these ponds, while the algae use the nutrients and carbon dioxide by bacterial decomposition, the bacteria make use of the oxygen liberated by the algae during photosynthesis, consequently increasing the rich natural biomass for the fishes. Updated compiled information in this review article suggests that domestic waste-water aquaculture is one of the best alternative ways to remove eutrophication as well as increase the culture of poly carps. This domestic sewage-purification cum reclamation bioprocess can be one of the cheapest methods, where natural sunlight, tropical conditions and biological parameters if managed judiciously, can be recycled and reclaimed for economically viable fish culture.
Ami D. Varia
Bioscience Biotechnology Research Communications, Volume 15, pp 407-418; https://doi.org/10.21786/bbrc/15.3.6

Abstract:
Haloalkaliphilic bacteria are a specific group of bacteria known to us. The diversity of microorganisms is critical to the functioning of the ecosystem as there is the need to maintain ecological process such as decomposition of organic matter, nutrient cycling, soil aggregation and control of pathogens within the ecosystem. Microbial diversity as an indicator of the quality of agroecosystems has been widely debated, In the present study various saline soil samples collected from Bhavnagar and Uncha Kotda, Gujarat, India. The collected five samples were analysed for diversity study of soil sample for physicochemical analysis like pH, redox potential, conductivity, humidity, salinity and soil analysis for total nitrogen and organic carbon analysis also. Total 55 haloalkaliphilic bacterial morphotypes were isolated and screened for alkaline protease production on halophilic agar medium. Out of them 76.4% gram positive bacilli, 21.8% gram positive cocci and 0.0002% were gram negative short rods. From total 55 morphotypes 33% were different morphotypes, 8% were zone of casein producer, and 16% of them were pigment producing morphotypes. All the pigmented colony producer morphotypes showed growth of orange, yellow, red, light pink and light-yellow colonies on 15%, 20%, 25%, 30% NaCl containing medium. Dominantly bacilli were found in all five samples. Diversity indices for metabolic characterization studies like Shannon-weiner index (H’), Richness, Evenness, Cho-1, Simpson’s index and Good’s coverage were calculated based on the site wise obtained different morphotypes. Phenotypic characteristics were studied. The secondary screening and tertiary screening were done on the basis of REA and different NaCl concentration accordingly. Identification of all haloalkaliphilic protease producers were confirmed by 16S r-RNA identification.
Bioscience Biotechnology Research Communications, Volume 15, pp 442-449; https://doi.org/10.21786/bbrc/15.3.11

Abstract:
The potential microalgal strains that are robust and display high growth and lipid accumulation rates are an important prerequisite for using them as a bioenergy source. We have isolated and screened six morphologically different microalgae strains, isolated from the Sabarmati River in Gujarat, India. The growth rates and lipid productivity of all six microalgae were assessed. Three potential microalgae strains were screened based on maximum biomass and lipid production. We named them MA001, MA002 and MA003. We identified MA001 as Micractinium reisseri based on its 28S rRNA sequencing. The M. reisseri showed an optimal growth rate of 2 g/L (dry weight) and 52 % lipid content after 20 days of cultivation in a normal artificial saline medium. Further analysis of lipid accumulation in M. reisseri was investigated at different concentrations of nitrogen. M. reisseri accumulated the highest amount of lipid under nitrogen starvation.
Bioscience Biotechnology Research Communications, Volume 15, pp 431-434; https://doi.org/10.21786/bbrc/15.3.9

Abstract:
The increase in number of Postpartum Depression (PPD) cases in Kerala is increasing day by day. So, a study on the awareness of PPD has become necessary in this scenario. This study tries to quantify the awareness among women in Kerala about PPD and covers how they tackled the issue through various methods used by them to cope with the issue. The study is conducted using an online survey method. A prepared questionnaire was circulated online among 150 young mothers who were born and brought up in Kerala. The questionnaire consisted of eight questions about PPD and baby blues. Each question was provided with options from which the participants had to choose one. The data thus collected were studied and analyzed. The results of the survey were analyzed to arrive at a conclusion. There was active participation from the participants’ side. The results showed that the percentage of participants who were well aware of PPD was very low. The study brought to light that, much attention and activities are needed to solve PPD cases in Kerala. Most women who suffer from PPD are reluctant to seek medical help. This situation can change through proper campaigns and other related activities.
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