Journal Buletin Plasma Nutfah

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Untung Setyo-Budi, B. Heliyanto, Nfn Sudjindro
Buletin Plasma Nutfah, Volume 10; doi:10.21082/blpn.v10n2.2004.p77-81

Abstract:Exploration was conducted to be used for genetic variation improvement of abaca germplasm. It was performed at Sangir Besar Island in Sangihe Achipilago and Karakelang Island in the Talaud Archipilago, both of them were located in the teritorial of Sangihe-Talaud District of North Sulawesi Province, in September 1999. Result indicated that Sangihe-Talaud District had many species of abaca introduced from Philippina. Fifteen accessions of abaca were collected. The difference characters of each accession were identified by stem colour, bloom shape and colour, stem height and diameter, and fibre strength. Those accessions were conserved in the RITFC’s Experimental Garden for characterization and evaluation.AbstrakUntuk memperluas keragaman sumber daya genetik tanaman abaca, telah dilakukan eksplorasi di Pulau Sangir Besar di gugusan Kepulauan Sangihe, dan di Pulau Karakelang di gugusan Kepulauan Talaud, Kabupaten Sangihe-Talaud, Propinsi Sulawesi Utara, pada bulan September 1999. Dari eksplorasi tersebut diketahui bahwa di Kabupaten Sangihe-Talaud, Propinsi Sulawesi Utara khususnya di Pulau Sangir Besar dan Pulau Karakelang, banyak terdapat jenis abaca yang diduga berasal dari Filipina. Diperoleh 15 aksesi abaca dari kedua pulau tersebut. Perbedaan yang mencolok dari masing-masing aksesi terletak pada karakter warna batang, warna dan bentuk jantung, tinggi dan diameter batang serta kekuatan seratnya. Aksesi-aksesi tersebut ditanam di kebun plasma nutfah Balittas untuk karakterisasi dan evaluasi.
Imron Riyadi, Nfn Tirtoboma
Buletin Plasma Nutfah, Volume 10; doi:10.21082/blpn.v10n2.2004.p82-89

Abstract:Direct induction of somatic embryos in Arabica coffee (Coffea arabica L.) using plant groth regulators (PGR's) has been successful. The concentration and combination of different kinds of PGR's can influence the response and success in embryo induction. An experiment was conducted to determine the optimal concentration of 2.4-D in combination with kinetin for direct induction and proliferation of somatic embryos. The plant material used was Arabica coffee var. Kartika-l originating from The Indonesian Coffee and Cacao Research Institute, Jember. Explants were taken from young leaves of reddish-green in color. Somatic embryos were induced directly on a Murashige-Skoog (MS) standard medium containing 30 g/l sucrose and supplemented with 0, 1, 2, 4, and 8 mg/l 2.4-D in combination with 0.1 mg/l kinetin each. The cultures were incubated in the dark at temperature 26oC and RH +60% for 6 weeks with 10 replications. The results showed that somatic embryogenesis in Arabica coffee was best induced in a culture medium wiyh 2.4-D at 4 mg/l, combination with 0.1 mg/l kinetin. Induction of somatic embryos was achieved at 100% 4 weeks after culture. Three morphological stages of embryo development were identified: globular, early heart, and middle heart. The embryos were of three distinct colors such as, yellowish, yellowish-white, and white. The highest rate of proliferation of somatic embryos was achieved at 2 mg/l, 2.4-D in combination with 0.1 mg/l kinetin averaging 68.53 embryos per explant 6 weeks after subculture.
Nfn Subandriyo
Buletin Plasma Nutfah, Volume 10; doi:10.21082/blpn.v10n2.2004.p90-100

Abstract:There are several sources of publication could be used as database. The sources could be articles that have been published in the periodicals as well as unpublished data (idle data). Database manager should be the person who know the background, objectives, and the principles to manage the data. In addition, the coordinators should be the persons who have background of animal geneticist by training. Data on the characteristics of breeds or strain within species are extracted and presented on specific format. The format are either in the free or fixed format, for computerization. The presentation could be separated into two form of Master Records containing physical characteristics, and Slave Records containing performance characteristics and environment. Each breed or strain has only one Master Record, but it could have several Slave Records. The number of slave records are dependent upon the number of publication available.AbstrakDi dalam pembuatan pangkalan data (database) yang merupakan bank data, bermacam-macam sumber dapat digunakan, mulai dari artikel yang telah dipublikasi maupun data yang tidak dipublikasi (idle data). Pengelola database harus mengetahui latar belakang, tujuan serta prinsip-prinsip dasar penanganan data. Koordinatornya paling tidak, telah mendapat pendidikan di bidang pemuliaan ternak (animal geneticist by training). Data karakteristik suatu rumpun (breed) atau strain dalam spesies diekstrak dan dipresentasikan dalam suatu format, baik dalam bentuk bebas (free format) maupun tetap (fixed format), sehingga mudah untuk dilakukan komputerisasi. Presentasinya dibedakan menjadi dua, yakni dalam bentuk karakteristik fisik (master record) serta untuk karakteristik performa dan lingkungan (slave record). Setiap rumpun atau strain mempunyai sebuah master record dan beberapa slave record. Jumlah slave record bergantung pada jumlah publikasi yang tersedia.
Mamik Setyowati, Nfn Hadiatmi, Nfn Sutoro
Published: 31 December 2007
Buletin Plasma Nutfah, Volume 11; doi:10.21082/blpn.v11n2.2005.p41-48

Abstract:Sorghum had been cultivated by farmers in certain area for a long time. It is used for food or feed and its production was relatively low. Germplasm collection have to be evaluated to obtain certain character which were important to plant breeding. The purpose of this study was to evaluate ratooning ability of sorghum. Research was conducted in Cikeumeuh Experiment Station, Bogor from April-December 1999 using randomized complete block design. There were three type of maturity were studied (21 accessions, 44 accessions and 35 accessions for short, medium and long maturity, respectively). Result showed that main crop was better than ratoon crops. Grain yield of accession No. 15/226 classified as short maturity in ratoon crop, was better than main crop, but total grain yield (main and ratoon crop) was not significantly different compare to Keris M3 and ICSV 93003. AbstrakTanaman sorgum telah lama dibudidayakan petani di daerah tertentu. Sorgum dapat digunakan sebagai pangan atau pakan dan umumnya memiliki produksi relatif rendah. Koleksi plasma nutfah perlu dievaluasi untuk mendapatkan karakter tertentu yang penting untuk pemuliaan tanaman. Tujuan dari penelitian ini adalah untuk mengevaluasi kemampuan ratoon dari plasma nutfah sorgum. Penelitian dilaksanakan di Instalasi Percobaan Cikeumeuh Bogor yang berlangsung pada April- Desember 1999 dengan menggunakan rancangan acak kelompok. Umur masak tanaman sorgum yang dipelajari terdiri dari 3 kelompok umur panen (21 aksesi umur pendek, 44 aksesi umur sedang, dan 35 aksesi umur panjang). Hasil penelitian menunjukkan bahwa tanaman utama lebih baik daripada tanaman ratoon. Bobot biji aksesi No. 15/226 lebih baik daripada tanaman utama, tetapi total bobot biji tanaman utama dan ratoon tidak berbeda nyata apabila dibandingkan dengan aksesi Keris-M3 dan ICSV 93003.
Sri Gajatri Budiarti
Buletin Plasma Nutfah, Volume 13; doi:10.21082/blpn.v13n1.2007.p1-10

Abstract:In December 2005, maize germplasm collection at the institute were 886 accessions consisted of 581 local varieties, 165 introduced varieties, 107 inbred, lines, and 33 old and the present improved varieties. Required traits in varietal improvement or development are drought tolerant, tolerant to Al-toxicity, early maturity, have better quality, resistant to downy mildew disease, resistant to insect Atherigona exigua and high yield. Maize germplasm must be characterized accordingly. Evaluation for Al toxicity was conducted at Tamanbogo, Lampung, 100-200 accessions of maize germplasm and tolerance to drought were conducted in Jakenan and Imogiri Central Java, 63-100 accessions. Evaluation for downy mildew resistance was conducted at Cikeumeuh Bogor, 100-200 accessions. Evaluation for resistance to seedingfly was conducted at Cikeumeuh, 75-100 accessions. The results of evaluation conducted from 1999-2004 were: 30 accessions tolerant to drought, 21 accessions. tolerant to Al-toxicity, 70 accessions resistant to downy mildew disease (Peronosclerospora maydis), 22 accessions resistant to seeding fly (Atherigona exigua), 126 accessions had very early maturity <80 days (consisted of 31 accessions had yellow seed, 35 accessions had white seed, and 60 accessions had other colour seed). Accessions having good characters, need to be further tested prior to their use in the breeding program, particularly the accessions resistant to Atherigona exigua in monoculture test. AbstrakSampai Desember 2005 koleksi plasma nutfah jagung di bank gen BB-Biogen berjumlah 886 aksesi yang terdiri dari 581 varietas lokal, 165 varietas introduksi, 107 galur inbrida, dan 33 varietas unggul lama dan baru. Sifat-sifat yang diinginkan dalam perakitan varietas antara lain adalah toleran kekeringan dan keracunan Al, berumur genjah, mutu gizi baik, dan tahan terhadap penyakit bulai, hama lalat bibit, dan hasil tinggi. Untuk tujuan tersebut, plasma nutfah perlu dikarakterisasi dan dievaluasi. Uji toleransi keracunan Al dilakukan di Tamanbogo, Lampung terhadap 100-200 aksesi. Uji kekeringan dilakukan di Jakenan dan Imogiri, Jawa Tengah terhadap 63-100 aksesi. Uji ketahanan penyakit bulai dilakukan di Cikeumeuh, Bogor terhadap 100-200 aksesi. Pengujian ketahanan terhadap lalat bibit dilakukan di Cikeumeuh terhadap 75-100 aksesi. Hasil pengujian sejak tahun 1999-2007 diperoleh informasi sebagai berikut: sebanyak 30 aksesi mempunyai sifat toleran terhadap kekeringan, 21 aksesi toleran keracunan Al, 70 aksesi sangat tahan penyakit bulai (Peronosclerospora maydis), dan 22 aksesi tahan terhadap hama lalat bibit (Atherigona exigua), 126 aksesi berumur sangat genjah (<80 hari). Aksesi-aksesi tersebut perlu diuji kembali kemantapan sifatnya sebelum digunakan dalam program pemuliaan, khususnya untuk aksesi yang tahan hama lalat bibit perlu diuji secara monokultur.
Nfn Kusmana, Eri Sofiari
Buletin Plasma Nutfah, Volume 13; doi:10.21082/blpn.v13n1.2007.p27-33

Abstract:Characterization was carried out in Indonesian Vegetable Reserach Institute (IVEGRI), Lembang. Twenty plants/plot and three replications for each variety were arranged in Randomized Block Design. The objective of the experiment was to compile characters of potato varieties Granola, Atlantic, and Balsa by implementing UPOV methods. The result shown in the form of table containing descriptions of 50 characters of Granola, Atlantic, and Balsa varieties. AbstrakKegiatan karakterisasi dilakukan di Balai Penelitian Tanaman Sayuran Lembang, pada ketinggian tempat 1250 m di atas permukaan laut. Populasi tanaman untuk masing-masing varietas adalah 20 tanaman yang diulang sebanyak 3 kali dengan menggunakan Rancangan Acak Kelompok. Tujuan kegiatan ini adalah untuk menerapkan metode baku UPOV mengenai penyusunan karakterisasi tanaman kentang pada varietas yang populer, yaitu Granola, Atlantic, dan Balsa, sehingga didapatkan informasi morfologi yang lengkap tentang varietas tersebut. Hasil kegiatan ini adalah karakterisasi dalam bentuk tabel yang berisi deskripsi 50 sifat varietas kentang Granola, Atlantic, dan Balsa.
N. M. Heriyanto, Reny Sawitri, Didi Subandinata
Buletin Plasma Nutfah, Volume 13; doi:10.21082/blpn.v13n1.2007.p34-42

Abstract:The study of population and distribution of saninten (Castanopsis argentea (Bl.) A.DC) seedling was conducted in August 2003 at block Pasarean, Cibodas Resort, Gede Pangrango National Park. The square inventory of 20 plots sizing at 2 x 2 m for seedling level and 20 x 20 m for tree level. Regeneration analysis were laid down at 1,300, 1,400, 1,500, 1,600, and 1,700 m asl. The research showed that the species composition of seedling communities at 1,300 and 1,600 m asl was kileho (Saurauia pendula Bl.), at 1,400 and 1,700 m asl was huru (Litsea sp.) and at 1,500 m asl was nangsi (Villebrunea sp.). The highest density distribution of tree and seedling stage of saninten were at 1,400 m asl which were 18 trees/ha and 833 seedlings/ha with dominance index of 0.06. While the highest of species diversity index occured at 1,300 m asl about 3.34 with 35 invidual species. More over, based on altitudinal sites, the value of index similarity was occured between 1,600 m asl and 1,700 m asl was 65.24%. AbstrakKajian populasi dan penyebaran anakan saninten (Castanopsis argentea (Bl.) A.DC) dilakukan pada Agustus 2003 di blok Pasarean, Resort Cibodas, Taman Nasional Gunung Gede Pangrango. Pengumpulan data dilakukan dengan menggunakan metode kuadrat bujur sangkar 2 x 2 m, sedangkan untuk tingkat pohon hanya jenis saninten. Ukuran petak yang digunakan 20 x 20 m. Petakan untuk semai terletak di salah satu sudut dalam petak untuk pengukuran pohon. Penelitian dilakukan pada ketinggian tempat 1.300, 1.400, 1.500, 1.600, dan 1.700 m, dpl. Petakan dibuat pada setiap ketinggian tempat sebanyak 20 petak. Hasil penelitian menunjukkan bahwa jenis yang mendominasi komunitas tumbuhan tingkat semai adalah kileho (Saurauia pendula Bl.) yang mendominasi pada ketinggian 1.300 dan 1.600 m dpl, huru (Litsea sp.) mendominasi semai pada ketinggian 1.400 dan 1.700 m dpl, dan nangsi (Villebrunea sp.) mendominasi semai pada ketinggian 1.500 m dpl. Penyebaran terluas pohon dan semai saninten pada ketinggian tempat 1.400 m dpl, yaitu 18 pohon dan 833 anakan/ha. Nilai dominansi tertinggi tingkat semai dimiliki oleh ketinggian tempat 1.400 m dpl dengan indeks dominansi 0,06. Nilai indeks keanekaragaman jenis tertinggi dimiliki oleh ketinggian tempat 1.300 m dpl yang mempunyai keanekaragaman jenis sebesar 3,34 dengan 37 jenis individu. Nilai kesamaan komunitas (IS) tertinggi komunitas semai terdapat antara ketinggian tempat 1.600 dan 1.700 m dpl sebesar 65,24%.
Sri Gajatri Budiarti
Published: 31 December 2007
Buletin Plasma Nutfah, Volume 11; doi:10.21082/blpn.v11n2.2005.p49-54

Abstract:The aim of the experiment were to rejuvenate and characterize of wheat germplasm. Eighty nine genotypes planted at Kuningan Field Research Instalation, West Java from June to October 2002. Plot size for each genotypes 3 x 1 m2 with spacing 25 x 10 cm. Seeds were planted with 2 seed per hole and thinned out to one plant. Several agronomic characters were observed: date of flowering, date of maturing, plant height, number of productive tiller per hill, seed weight per hill and yield per plot. The results showed that: date of flowering (mean 60.9 days with range 48-77 days), date of maturity (mean 102.7 days with range 87-119 days), plant height (mean 72.4 cm, range 53.5-88.7 cm), number of productive tiller per hill (mean 9.71, range 4.9-24.0), seed weight per hill (mean 11.96 g, range 5.3-34.5 g) and seed yield per plot (200.0- 2624.5 g). Seventeen genotypes have date of flowering 17.5, some of them were C8 (23.6), C27 (22.7) and the greatest number was C28 (24.0). Sixty six genotypes have number of productive tiller per hill, ranged 4.9-11.2, some of them were: C34 (4.9), C24 (5.2), H85 (5.6). Seed weight per hill showed that 60 genotypes have seed weight 2000 g were V192 (2016.0 g), and V167 (2624.5 g). AbstrakTujuan penelitian adalah merejuvenasi dan mengkarakterisasi plasma nutfah gandum, terutama morfologi dan agronomi. Penelitian dilaksanakan pada Juni hingga Oktober 2002 di Inlitpa Kuningan, Jawa Barat. Sejumlah 89 genotipe gandum ditanam pada petak berukuran 3 x 1 m2, dengan jarak tanam 25 x 10 cm, secara tugal, dua biji per lubang, dan diperjarang menjadi satu tanaman. Pemupukan dilakukan pada 1 MST secara alur di samping barisan tanaman. Pupuk dasar terdiri dari 100 kg urea, 200 kg SP36, dan 50 kg KCl/ha. Sejumlah 100 kg urea/ha diberikan lagi pada umur 5 MST dan 9 MST. Karakterisasi dilakukan terhadap umur berbunga, umur masak, tinggi tanaman, jumlah anakan produktif per rumpun, bobot biji per rumpun, dan hasil biji per petak. Umur berbunga ratarata 60,9 hari dengan rentang 48-77 hari, umur masak 102,7 hari dengan rentang 87-119 hari, tinggi tanaman 72,4 cm dengan rentang 53,5-88,7 cm, jumlah anakan produktif per rumpun 9,71 dengan rentang 4,9-24, bobot biji per rumpun 11,96 g dengan rentang 5,3-34,5 g, dan hasil biji per petak berkisar antara 200-2624,5 g. Genotipe yang mempunyai umur berbunga 17,5 di antaranya adalah C28, C8, C27. Genotipe yang mempunyai jumlah anakan produktif 4,9-11,2 di antaranya adalah C34, C24, dan H85. Genotipe yang mempunyai bobot biji 2000 g adalah V192 dan V167.
Ahmad Sulle
Buletin Plasma Nutfah, Volume 13; doi:10.21082/blpn.v13n1.2007.p19-26

Abstract:Cashew and cocoa are main commodities in South-East Sulawesi. Main problem of cocoa is cocoa pod borer (CPB) which to date, no effective control was found. Used of resistant variety is recommended control measure. The result of individual identification in field were: BPTP.R1.97, BPTP.R2.97, BPTP.R3.97, BPTP.W1.97, BPTP.W2.97, and BPTP.W3.97 resistant to CPB. Others having high yield i.e.: GC 7, ICS 60, ICS 13, UIT 1, Pa 300, TSH 858, RCC 70, RCC 71, RCC 72, and RCC 73. The result of side grafting trial at smallholder plantation the best clones were UIT-1, Na-32, ICS-13, Pa-7, RCC-70, RCC71, and BR-25. It is recommended for enters to side grafting the control to CBB. Problem of Cashew farming is low productivity and rainfall fluctuation. Individual selection on muna type 95 accessions were high productivity and 28 of the other were tolerant to rainfall fluctuation. Sixty two cashew accessions were collected. Six accessions were planted in commercial plantation. Superior clones of cocoa and cashew nut will be used for new planting and rehabilitation of old crop. AbstrakJambu mete dan kakao merupakan komoditas unggulan di Sulawesi Tenggara. Masalah utama pada kakao adalah serangan hama penggerek buah kakao (PBK) yang sampai saat ini belum ditemukan cara penanggulangan yang efektif dan efisien. Pilihan utama yang prospektif adalah menggunakan bahan tanam yang tahan. Hasil identifikasi di lapang ditemukan individu BPTP.R1.97, BPTP.R2.97, BPTP.R3.97, BPTP.W1.97. BPTP.W2.97, dan BPTP.W3.97 yang tahan terhadap hama PBK. Selain itu, telah dianjurkan menanam GC 7, ICS 60, ICS 13, UIT 1, Pa 300, TSH 858, RCC 70, RCC 71, RCC 72, dan RCC 73. Uji sambung samping kakao di pertanaman rakyat menghasilkan 7 nomor yang memiliki daya sambung amat baik dan baik yaitu UIT-1, Na-32, ICS-13, Pa-7, RCC-70, RCC71, dan BR-25. Masalah pada usahatani mete adalah produktivitas rendah dan berfluktuasi karena curah hujan yang tinggi. Dari seleksi individu pada tipe muna ditemukan 95 nomor berproduksi tinggi dan 28 nomor di antaranya toleran terhadap fluktuasi curah hujan. Koleksi Jambu mete mengsilkan 62 nomor aksesi. Enam nomor yang diunggulkan ditanam dalam kebun entres komersil. Klon-klon unggul kakao dan mete tersebut layak digunakan sebagai bahan tanam untuk pengembangan baru dan merehabilitasi tanaman tua.
Yenni Kusandriyani, Nfn Luthfy, Nfn Gunawan
Published: 31 December 2007
Buletin Plasma Nutfah, Volume 11; doi:10.21082/blpn.v11n2.2005.p55-59

Abstract:Characterization of tomato germplasm on the basis of tomato description was conducted of the Lembang Vegetables Government Research Institute. (1250 m asl), in the dry season 2003. Twenty five accession were observed, each accessions 20 plants were planted per plot with the distance 70 x 50 cm. Plants were fertilizer at the rate of 30 t/ha stable manure and 1000 kg/ha NPK applied two days before planting. Description of those accessions was determined for further use in breeding programme. AbstrakKegiatan karakterisasi dilakukan untuk mendapatkan deskripsi sifat tanaman untuk dimanfaatkan sebagai bahan working collection dalam program pemuliaan. Deskripsi merupakan ciri dari sifat tanaman yang dikoleksi. Penelitian menggunakan 25 aksesi, ditanam di kebun percobaan Lembang (1250 m dpl) pada musim kemarau 2003, masing-masing aksesi ditanam 20 tanaman per petak dengan jarak tanam 70 x 50 cm. Tanaman dipupuk dengan pupuk kandang kuda dengan takaran 30 t/ha dan NPK 1000 kg/ha. Pupuk kandang diberikan dua hari sebelum tanam dan NPK pada saat tanam. Hasil penelitian menghasilkan deskripsi pertumbuhan dan komponen hasil.
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