Journal International Journal of Bioprinting

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Cijun Shuai, Youwen Yang, Pei Feng, Shuping Peng, Wang Guo, Anjie Min, Chengde Gao
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.133

Abstract:It is critical to develop a fabrication technology for precisely controlling an interconnected porous structure of scaffolds to mimic the native bone microenvironment. In this work, a novel combined process of additive manufacturing (AM) and chemical etching was developed to fabricate graphene oxide/poly(L-lactic acid) (GO/PLLA) scaffolds with multi-scale porous structure. Specially, AM was used to fabricate an interconnected porous network with pore sizes of hundreds of microns. And the chemical etching in sodium hydroxide solution constructed pores with several microns or even smaller on scaffolds surface. The degradation period of the scaffolds was adjustable via controlling the size and quantity of pores. Moreover, the scaffolds exhibited surprising bioactivity after chemical etching, which was ascribed to the formed polar groups on scaffolds surfaces. Furthermore, GO improved the mechanical strength of the scaffolds.
Chee Kai Chua
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.131

Tae-Sik Jang, Hyun-Do Jung, Houwen Matthew Pan, Win Tun Han, Shenyang Chen, Juha Song
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.126

Abstract:Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and promote cell infiltration for tissue engineering. However, pure hydrogels alone lack the necessary mechanical stability and are too easily degraded to be used as printing ink. To overcome this problem, significant progress has been made in the 3D printing of hydrogel composites with improved mechanical performance and biofunctionality. Herein, we provide a brief overview of existing hydrogel composite 3D printing techniques including laser based-3D printing, nozzle based-3D printing, and inkjet printer based-3D printing systems. Based on the type of additives, we will discuss four main hydrogel composite systems in this review: polymer- or hydrogel-hydrogel composites, particle-reinforced hydrogel composites, fiber-reinforced hydrogel composites, and anisotropic filler-reinforced hydrogel composites. Additionally, several emerging potential applications of hydrogel composites in the field of tissue engineering and their accompanying challenges are discussed in parallel.
Yannapol Sriphutkiat, Surasak Kasetsirikul, Yufeng Zhou
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.130

Abstract:3D bioprinting becomes one of the popular approaches in the tissue engineering. In this emerging application, bioink is crucial for fabrication and functionality of constructed tissue. The use of cell spheroids as bioink can enhance the cell-cell interaction and subsequently the growth and differentiation of cells in the 3D printed construct with the minimal amount of other biomaterials. However, the conventional methods of preparing the cell spheroids have several limitations, such as long culture time, low-throughput, and medium modification. In this study, the formation of cell spheroids by SSAW was evaluated both numerically and experimentally in order to overcome the aforementioned limitations. The effects of excitation frequencies on the cell accumulation time, diameter of formed cell spheroids, and subsequently, the growth and viability of cell spheroids in the culture media over time were studied. Using the high-frequency (24.9 MHz) excitation, cell accumulation time to the pressure nodes could be reduced in comparison to that of the low-frequency (10.4 MHz) excitation, but in a smaller spheroid size. SSAW excitation at both frequencies does not affect the cell viabilities up to 7 days, > 90% with no statistical difference compared with the control group. In summary, SSAW can effectively prepare the cell spheroids as bioink for the future 3D bioprinting and various biotechnology applications (e.g., pharmaceutical drug screening and tissue engineering).
Fan Liu, Chen Liu, QIUHONG CHEN, QIANG AO, Xiaohong Tian, Jun Fan, Hao Tong, XIAOHONG WANG
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.128

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Mehri Behbehani, Adam Glen, Caroline S. Taylor, Alexander Schuhmacher, Frederik Claeyssens, John W. Haycock
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.123

The publisher has not yet granted permission to display this abstract.
Youwen Deng, Youwen Yang, Chengde Gao, Pei Feng, Wang Guo, Chongxian He, Jian Chen, Cijun Shuai
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.124

Abstract:It remains the primary issue to enhance the corrosion resistance of Mg alloys for their clinical applications. In this study, β-tricalcium phosphate (β-TCP) was composited with Mg-6Zn-1Zr (ZK60) using laser rapid solidification to improve the degradation behavior. Results revealed rapid solidification effectively restrained the aggregation of β-TCP, which thus homogenously distributed along grain boundaries of α-Mg. Significantly, the uniformly distributed β-TCP in the matrix promoted the formation of apatite layer on the surface, which contributed to the formation of a compact corrosion product layer, hence retarding the further degradation. Furthermore, ZK60/8β-TCP (wt. %) composite showed improved mechanical strength, as well as improved cytocompatibility. It was suggested that laser rapidly solidified ZK60/8β-TCP composite might be a potential materials for tissue engineering.
Hongtao Liang, Jiankang He, Jinke Chang, Bing Zhang, Dichen Li
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.127

Abstract:Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements. However, it is still challenging to print cell-laden 3D structures simultaneously with high resolution and high cell viability. Here a coaxial nozzle-assisted electrohydrodynamic cell printing strategy was developed to fabricate living 3D cell-laden constructs. Critical process parameters such as feeding rate and stage moving speed were evaluated to achieve smaller hydrogel filaments. The effect of CaCl2 feeding rate on the printing of 3D alginate hydrogel constructs was also investigated. The results indicated that the presented strategy can print 3D hydrogel structures with relatively uniform filament dimension (about 80 μm) and cell distribution. The viability of the encapsulated cells was over 90%. We envision that the coaxial nozzle-assisted electrohydrodynamic printing will become a promising cell printing strategy to advance biomedical innovations.
Cijun Shuai, Wang Guo, Chengde Gao, Youwen Yang, Ping Wu, Pei Feng
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v4i1.120

Abstract:Bone repair failure caused by implant-related infections is a common and troublesome problem. In this study, an antibacterial scaffold was developed via selective laser sintering with incorporating nano magnesium oxide (nMgO) to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The results indicated the scaffold exerted high antibacterial activity. The antibacterial mechanism was that nMgO could cause oxidative damage and mechanical damage to bacteria through the production of reactive oxygen species (ROS) and direct contact action, respectively, which resulted in the damage of their structures and functions. Besides, nMgO significantly increased the compressive properties of the scaffold including strength and modulus, due to its excellent mechanical properties and uniform dispersion in the PHBV matrix. Moreover, the degradation tests indicated nMgO neutralized the acid degradation products of PHBV and benefited the degradation of the scaffold. The cell culture demonstrated that nMgO promoted the cellular adhesion and proliferation, as well as osteogenic differentiation. The present work may open the door to exploring nMgO as a promising antibacterial material for tissue engineering.
Eric Lepowsky, Savas Tasoglu
International Journal of Bioprinting, Volume 4; doi:10.18063/ijb.v1i1.119

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