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Journal Applied Environmental Biotechnology

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39 articles
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Jian-Lan Wang, Wen Gu, Shi-Fa Wang
Applied Environmental Biotechnology, Volume 4; doi:10.26789/aeb.2019.01.002

Abstract:New N-alkylfurfurylacetamides were synthesized and characterized from abundant biomass derived furfural by a simple and straightforward procedure with overall yields of 51 to 92%. The antimicrobial activity of the synthesized compounds were then investigated and it was found that N-benzyl-N-furfurylacetamide and N-cyclohexyl-N-furfurylacetamide showed promising activity against bacteria, particularly white-rot-fungi, suggesting a feasible new type of anti-fungal agent in applications to protection and conservation of cultural heritage.
Yong-Feng Li
Applied Environmental Biotechnology, Volume 4, pp 11-17; doi:10.26789/aeb.2019.01.003

Abstract:The depletion of non-renewable resources and pollution of industrial wastewater are major challenges to the human security. Using green renewable resources to address the above problems coincides with the sustainable development of human society. In this study, we attend to design hybrid aerogel, derived from nanocellulose and graphene oxide (GO), to realize wastewater purification via adsorption behavior, benefitting from its high specific surface area and high porosity. Nanocellulose, isolated from Amorpha fruticosa Linn. as a shrub plant, and graphene oxide were combinely employed to prepare the hybird aerogel via freeze-drying process; and its purification ability to remove methylene blue(MB), congo red (CR) and waste oil in waste water was tested. The results indicate that the isolated nanocellulose bears abundant hydroxyl groups and high aspect ratio of ~500 with average diameter of ~30 nm, which is well distributed on the surface of graphene oxide sheet with side length of about 1~3 μm, both of which form the hybrid aerogel with porosity larger than 99%. The nanomaterials physically assemble its orignial aggregation state. When the mass ratio of nanocellulose and graphene oxide is 8 : 2, the hybrid aerogel reaches the highest adsorption capacity of 265.6mg/g and 21.5mg/g for MB and CR, respectively. The hybrid aerogel after hydrophobic treatment shows excellent oil adsorption capacity up to 25.6 g/g, which is beneficial to oil/water separation. This strategy provides potential great-application of the nanocellulose in water purification.
Ji-Dong Gu
Applied Environmental Biotechnology, Volume 4, pp 1-4; doi:10.26789/aeb.2019.01.001

Abstract:Microorganisms play an important role in cycling of elements of ecosystems, including a wide range of chemical pollutants from anthropogenic origin. These pollutants in ecosystems, particularly aquatic, and sediment and soils, are in different physical and chemical forms in association with the inorganic and organic constituents of the sediment and soils, resulting in variable availability of them to microorganisms for assimilation and transformation. A thorough and comprehensive knowledge of the physical and chemical states of them in the environments requires detailed information of both the bioavailable pollutant concentration and also the metabolic capability of the microorganisms to assess the ecological and environmental toxicity of these pollutants meaningfully. Apart from the primary role as decomposers, microorganisms are qualified to be sensitive indicators for environmental pollution, and ecological health and ecotoxicity of pollutants because of their very short generation time and quickly response to chemical pollutants than higher and large organisms. When used for testing with the same strain, different laboratories can generate high reproducible results to allow comparison of the data feasible, not mention the reduction in cost. Based on the current advances made on genomics analysis and bioinformatics, microbial genomes are easily assembled with the technologies available to providing useful transcriptomic and metabolic annotations, expression and prediction to allow advance toxicological to another level.
Zomesh Artus Nath Maini, Kiara Marie J. Aribal, Regine Marinelli A. Narag, Jeorgina Kamella Luanshya T. Melad, Juan Angelo D. Frejas, Luis Alfonso M. Arriola, Pia Clarisse Ramos Gulpeo, Ian A. Navarrete, Crisanto M. Lopez
Applied Environmental Biotechnology, Volume 4, pp 18-29; doi:10.26789/aeb.2019.01.004

Ji-Dong Gu
Applied Environmental Biotechnology, Volume 3, pp 1-22; doi:10.26789/aeb.2018.02.002

Abstract:Coastal ecosystem is important because it bridges ocean and land. The brackish water receiving nutrients originated from land may nourish heterotrophic bacteria including Vibrio species, some of which may pose potential hazards to the public, marine lives and migratory birds in the coastal environment. A rich diversity of Vibrios is evident in the coastal and open oceans, but information on their ecophysiological adaptation and survival is still very limited. Their important roles in the geobiochemical cycles of nutrients have not been exolored adequately. In addition, it also been recently discovered that these Vibrios harbor a very rich of plasmids of various sizes with little knowledge on their function to the hosts. This information deserves attention in Vibrio ecology and their role in the various ecosystems for a better understanding of their survival and physiological function.
Zhao Yanting, Ye Lin, Duan Cuilan, Zhang Xu-Xiang
Applied Environmental Biotechnology, Volume 3, pp 1-8; doi:10.26789/aeb.2018.02.001

Abstract:The lactic acid bacteria (LAB) play a crucial role in the health of aquatic animals through controlling and competing with pathogens. In this study, based on the high-throughput sequencing of 16S rRNA gene amplicons, we examined the LAB in the gut of freshwater shrimp (Macrobrachium nipponense) and their living environments (sediment and pond water) and analyzed the correlations between the shrimp production and abundance of LAB. A high diversity and abundance of LAB (27 genera) were observed among the freshwater shrimp gut samples, and the results indicated that dissolved oxygen and temperature could affect the LAB community in the shrimp gut. In addition, share and unique LAB among the shrimp gut, sediment and pond water were further analyzed. Linear regression analysis showed that the relative abundance of LAB was positively correlated with the levels of shrimp production. Moreover, comparison of the LAB community among different animals indicated that some LAB in shrimp gut may also play a beneficial role in fish, houseflies, pig and other animals. Collectively, this study provides comprehensive information for better understanding LAB in shrimp gut and their environments and further improving the ecological management of aquatic ecosystems regarding the application of probiotics and disease prevention.
Lin Na Du, Ke Ke Pan, Gang Li, Yu Yi Yang, Fang Cheng Xu
Applied Environmental Biotechnology, Volume 3, pp 1-7; doi:10.26789/aeb.2018.02.005

Abstract:Microbial decolorization has been investigated extensively. Various microbes have been studied for their dye removing capability; however, microbial decolorizer with a strong environmental adaptability and wide substrate spectrum is of great potential for its possible practical application. Therefore, in this study, Aeromonas sp. DH-6, a wide dye spectrum decolorizer, was investigated in terms of its use for Malachite Green (MG) remediation. Results indicated that most of carbon sources have no effect on decolorization, while the nitrogen sources of beef extract and yeast extract could enhance MG decolorization significantly. Among the tested metal ions, Cu2+, Fe2+, and Zn2+ could significantly inhibit decolorization. Moreover, the strain showed a very stable and efficient decolorization performance in the pH of 5.0-10.0 and at 20-40oC. Besides, it could almost completely decolorize MG at concentrations ≤ 1000 mg/L within 36 h. Based on UV-visible, GC-MS, and FTIR analysis, biodegradation of MG by the strain DH-6 was confirmed and data showed that MG was decomposed into 4-(Dimethylamino)benzophenone and other metabolites containing –C=O, –NH, and –OH groups. Enzyme analysis showed that tyrosinase, laccase, LiP, NADH-DCIP reductase, and MG reductase might be involved in MG degradation by the strain DH-6. Overall, the results demonstrated that the strain DH-6 will have an effective use as an alternative in MG bioremediation.
Chen-Yang Li, Fang-Fang Liu, Jiang Ye, Jin-Feng Liu, Shi-Zhong Yang, Hui-Zhan Zhang, Bo-Zhong Mu
Applied Environmental Biotechnology, Volume 3, pp 1-9; doi:10.26789/aeb.2018.02.004

Abstract:A low-temperature active endo-β-1,4-mannanase (YBMan) from Bacillus subtilis TD7 was isolated, characterized and successfully expressed in Escherichia coli to enhance the yield of mannanase for a potential application as a gel-breaker in guar gum-based fracturing fluids in oilfields. YBMan showed good compatibility with a wide temperature range and retained about 70% relative activity at 20°C compared to its optimal temperature (65°C). This is the highest relative activity among reported low-temperature active mannanases against guar gum. The gene (1104 bp) of strain TD7 coding a protein with 367 amino acid residues was cloned and its expression generated two recombinant mannanases, TBMan-1 and TBMan-2. Compared to the wild type, the protein yield of TBMan-1 from a one-liter shake flask broth increased 5.6-fold, and the specific activity (crude enzyme) increased 6.4-fold. The total enzyme activity increased 35.8-fold with a total activity of approximately 79550 U. Moreover, TBMan-1 had at 20°C still about 80% relative activity. The enzyme was evaluated also for its application as gel-breaker and showed excellent ability for viscosity reduction with guar gum at 20°C. Low-temperature activity and high yield make the recombinant β-mannanase attractive for applications with guar-based hydraulic fracturing fluids and other biotechnological aspects.
Ji-Dong Gu
Applied Environmental Biotechnology, Volume 3, pp 1-4; doi:10.26789/aeb.2018.02.006

Abstract:Pollution of soils and sediments by metals and metalloids is a serious environmental problem and threat to the ecological health and environmental quality. Microorganisms are known capable of detoxifying metals and metaloids into insoluble or non-bioavailable forms so that bioaccumualtion can be prevented under selective conditions. A key issue involved in bioremediation is the very poor understanding on the chemistry of the pollutants, specifically the bioavailable concentartions of metals and metalloids in the environmental matrices, especially soils and sediments and at the relevant pH value. Chemical states of the pollutants in terms of speciation are crucial to the possible success of any remediation practice, but it is impossible to conduct an effective operation for cleaning up without such information in mind. In the literature available, it is a common trend and practice to justify bioremediation for in situ application by using pure cultures of microorganisms, but this is a very prematured and bold attempt to applying microorganisms for in situ cleaning up without any scientific ground to support. For polluted soils and sediments, microorganisms have no role for cleaning up but phytoremediation is an effective means to remove and extract toxic metals and metallods from the complex soil matrices. This has been demonstrated successfully with a number of metals and organics as well as organic pollutants in both laboratory and also field trials.
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