ACS Applied Bio Materials

Journal Information
ISSN / EISSN : 2576-6422 / 2576-6422
Published by: American Chemical Society (ACS) (10.1021)
Total articles ≅ 2,492
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Michael C. Robitaille, Joseph A. Christodoulides, Patrick J. Calhoun, Jeff M. Byers,
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00797

Abstract:
Surface ligand activity is a key design parameter for successfully interfacing surfaces with cells—whether in the context of in vitro investigations for understanding cellular signaling pathways or more applied applications in drug delivery and medical implants. Unlike other crucial surface parameters, such as stiffness and roughness, surface ligand activity is typically based on a set of assumptions rather than directly measured, giving rise to interpretations of cell adhesion that can vary with the assumptions made. To fill this void, we have developed a concurrent control technique for directly characterizing in vitro ligand surface activity. Pairs of gold-coated glass chips were biofunctionalized with RGD ligand in a parallel workflow: one chip for in vitro applications and the other for surface plasmon resonance (SPR)-based RGD activity characterization. Recombinant αVβ3 integrins were injected over the SPR chip surface as mimics of the cellular-membrane-bound receptors and the resulting binding kinetics parameterized to quantify surface ligand activity. These activity measurements were correlated with cell morphological features, measured by interfacing MDA-MB-231 cells with the in vitro chip surfaces on the live cell microscope. We demonstrate how the interpretation of a cell phenotype based on direct activity measurements can vary markedly from interpretations based on assumed activity. The SPR concurrent control approach has multiple advantages due to the fact that SPR is a standardized technique and has the sensitivity to measure ligand activity across the most relevant range of extracellular surface densities, while the in vitro chip design can be used with all commonly used light microscopy modalities (e.g., phase contrast, DIC, and fluorescence) so that a wide range of phenotypic and molecular markers can be correlated to the ligand surface activity.
Ji-Hong Bong, Jun-Hee Park, Jeong Soo Sung, Chang Kyu Lee, Ga-Yeon Lee, Min-Jung Kang, Hyun Ok Kim,
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00538

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Wenbo Wang, Chang-Yu Hung, Leslie Howe, Jia Chen, Kaiwen Wang, Vinh X. Ho, Shannon Lenahan, , Nguyen Q. Vinh,
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00826

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Mengyuan Zhou, Xuemei Shi, Xiaobai Li, Gang Xiao, Liping Liang, Jun Ju, , , Wei Sun, , et al.
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00948

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Sudan Liu, Zirui Li, Qiuxiang Wang, Jing Han, Wenying Wang, Shenghui Li, , , , Kun Ge, et al.
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00967

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Samavi Farnush Bint E Naser, Hui Su, Han-Yuan Liu, Zachary A. Manzer, Zhongmou Chao, Arpita Roy, Anna-Maria Pappa, Alberto Salleo, ,
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00878

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Chiara Tullio, Lucia Salvioni, Michela Bellini, Anna Degrassi, Luisa Fiandra, , Stefania Garbujo, Rany Rotem, Filippo Testa, , et al.
ACS Applied Bio Materials; https://doi.org/10.1021/acsabm.1c00724

Abstract:
Magnetic resonance imaging (MRI) is one of the most sophisticated diagnostic tools that is routinely used in clinical practice. Contrast agents (CAs) are commonly exploited to afford much clearer images of detectable organs and to reduce the risk of misdiagnosis caused by limited MRI sensitivity. Currently, only a few gadolinium-based CAs are approved for clinical use. Concerns about their toxicity remain, and their administration is approved only under strict controls. Here, we report the synthesis and validation of a manganese-based CA, namely, [email protected] Manganese is an endogenous paramagnetic metal able to produce a positive contrast like gadolinium, but it is thought to result in less toxicity for the human body. Mn ions were efficiently loaded inside the shell of a recombinant H-ferritin (HFn), which is selectively recognized by the majority of human cancer cells through their transferrin receptor 1. [email protected] was characterized, showing excellent colloidal stability, superior relaxivity, and a good safety profile. In vitro experiments confirmed the ability of [email protected] to efficiently and selectively target breast cancer cells. In vivo, [email protected] allowed the direct detection of tumors by positive contrast enhancement in a breast cancer murine model, using very low metal dosages and exhibiting rapid clearance after diagnosis. Hence, [email protected] is proposed as a promising CA candidate to be developed for MRI.
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