Journal of Scientific Research in Medical and Biological Sciences

Journal Information
ISSN / EISSN : 2709-0159 / 2709-1511
Total articles ≅ 50
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Manal Mohamed Elsayed Ahmed, Walid Nazmy, Asmaa Noureen
Journal of Scientific Research in Medical and Biological Sciences, Volume 3, pp 17-28; https://doi.org/10.47631/jsrmbs.v3i1.441

Abstract:
Purpose: This study aimed to develop ELISA kits for the detection of meat species adulteration in raw and processed meat and evaluate its specificity and sensitivity.Method: We identify the unique peptide markers based on the proteomics approach. Subsequently, we prepared specific antisera for beef, pork, horse, sheep, chicken and developed ELISA kits.Results: Evaluation of the developed kits in testing crooked reference sample mixtures revealed that the developed  ELISA kits showed 100% specificity with no cross-reactivity detected for the non-target species. In terms of sensitivity, the developed ELISA kits were able to consistently detect pork in the sample mixtures and horses at levels down to 5% w/w. Although sheep and chicken were detected at levels as low as 1% w/w. The beef-specific ELISA test showed greater sensitivity. Conclusions: It is concluded that the developed ELISA kits are a promising tool but further studies are still required for validation of the developed kits and comparing its results with the commercially used kits before entry into the production phase.
Adamu Mohammed Saba, Muhammad Abdullahi, Baba John, Samuel Angulu, James Dogari
Journal of Scientific Research in Medical and Biological Sciences, Volume 3, pp 1-6; https://doi.org/10.47631/jsrmbs.v3i1.437

Abstract:
Purpose:The assessment of HIV rapid test kits commonly used was evaluated for optimum performance on the suspected patient. This study aims to determine the sensitivity and specificity of rapid test kits used in public hospitals in Minna, Niger State. Methods: A total of 300 gold positive and gold negative samples were analyzed. Three rapid test kits (Determine, Stat-Pak, and Uni-Gold) were tested against gold standard serum using the method provided by the manufacturers of these kits.Results: Determine kit gave result sensitivity of (100%), specificity (96.5%), while Stat-Pak sensitivity was (99%), specificity (98%) and Uni-Gold sensitivity (100%), specificity (99%). In all Uni-Gold has the highest performance followed by Stat-Pak and lastly, Determine, but the differences were not statistically significant (p>0.05). Conclusion: This evaluation provides evidence for reliable rapid test kits for HIV testing in Nigeria. However, the claim by the manufacturers that their kit was 100% sensitive and 100% specific remains doubtful which may be a market strategy. Therefore, it is pertinent on a routine basis to evaluate the rapid test kits in circulation to re-validate their performance before usage for accurate HIV testing.
Mohamed Salman, Ahmed M. Gamal, Ebeed Yassen Ebrahim, Hesham Hamed Refae
Journal of Scientific Research in Medical and Biological Sciences, Volume 3, pp 7-16; https://doi.org/10.47631/jsrmbs.v3i1.345

Abstract:
Purpose: The study aimed at presenting a comparison between the modalities of treatment different of floating knee injury at Aswan University Hospital. Materials and Methods:  This study is a prospective study including all of our 20 cases of floating knee injuries who were treated utilizing various treatment modalities at Aswan University Hospital between December 2018 and September 2019 with a follow-up period of 12 months Results:Based on the data analysis,  nailing is a better modality in floating knee injury (especially with diaphyseal long bone). Moreover, plating is a good choice for distal fractures, the external fixator is considered a choice for limb saving(as in popliteal ischemia, open fractures(OG3), and compartment syndrome). Conclusion: Management of floating knee injury is critical as floating knee injury is not like other fractures. Floating knee injuries are serious injuries with a high rate of complications. Besides being caused by high-energy trauma with extensive skeletal and soft tissue damage, they are also associated with potentially life-threatening injuries of the head, chest, and abdomen. There are multiple controversies in surgical management starting from choosing suitable fixation for each patient according to variable conditions.  Floating knee injury remains a challenging orthopedic problem in which regaining good knee function outcome is a major concern. Stable osteosynthesis to achieve rigid fixation and early mobilization should always be attempted.
Mohammad Ohid Ullah, Mst. Farzana Akter, Shahnaj Sultana Sathi, Ayesha Akter
Journal of Scientific Research in Medical and Biological Sciences, Volume 3, pp 29-36; https://doi.org/10.47631/jsrmbs.v3i1.457

Abstract:
Purpose: The study aims to examine the association between food habits/lifestyle and breast cancer using an unsupervised machine approach.Method: The dataset was collected from the hospitals of eight divisional cities in Bangladesh using a semi-structured questionnaire. Descriptive statistical tools and an unsupervised machine learning approach- Factor Analysis were used to analyze the data.Results: The highest numbers of breast cancer patients were observed in the Sylhet division, followed by the Dhaka and Khulna divisions. It is noted that, overall, left breast cancer patients outnumber right breast cancer patients. We found that betel nuts, beverages, beef/mutton, etc. are high commonalities, which indicates that these food habits are highly associated with breast cancer. Moreover, most of the patients can’t bear the cost of treatment. Conclusions: Itis concluded that most breast cancer patients are used to taking betel nuts and beverages that may cause this disease. Therefore, we should avoid unhealthy and junk foods.
Rasha T Hamza, Amira I Hamed, Basma B B Hassan, Wafaa O Ahmed
Journal of Scientific Research in Medical and Biological Sciences, Volume 2, pp 18-29; https://doi.org/10.47631/jsrmbs.v2i4.365

Abstract:
Purpose: The study examined the effect of antenatal steroids on thyroid functions in late preterm infants on the third to the seventh day of life. Patients and Methods: A comparative Cross-Sectional study was conducted on 75 neonates admitted to NICU in the first week of life. They were divided according to exposure to antenatal steroids into three groups. First group: exposed to complete course of ANS. Second group: exposed to partial course of ANS. The third group: not exposed to ANS. Serum samples were obtained from selected cases free T3, Free T4, and TSH levels in the third day of life, compared to the cut of levels currently available (the TSH reference range is (1.7 to 9.1 mU per L), T4 should be greater than( 10 mcg per dL). Using Eleusis and Cubase analyzers kits (Roche Diagnostics, Indianapolis, IN, USA) by ELISA (enzyme-linked immune sorbent assay) technique. Results: The study showed that there were significantly higher serum T4 levels in group 1 that was exposed to a complete course compared to group 2 (partial course) and 3 (Third group). There were no significant differences in serum TSH, T3 levels between groups. The current study found a decreased incidence of the ROP, NEC and BPD among group 1 as compared to the other two groups although was non statistically significant. In addition, the complete course of ANS had delayed the date of delivery (P=0.04) as compared to the partial course in the current study. Conclusion: Antenatal corticosteroids can influence thyroid function in late preterm infants as serum T4 was significantly higher in infants exposed to complete course compared to those who were exposed to partial course or did not receive antenatal corticosteroids.
Ahmed M. Abdul Hameed, Haney A. Farouk, Mohamed S. Fahmy
Journal of Scientific Research in Medical and Biological Sciences, Volume 2, pp 38-43; https://doi.org/10.47631/jsrmbs.v2i4.330

Abstract:
Purpose: The study aims to investigate the effectiveness of Hayman’s suture in the management of Atonic postpartum hemorrhage. Subjects and Method: The study includes 37 patients who developed severe atonic PPH not responsive to pharmacological measures at the Department of Obstetrics and Gynecology at Aswan University hospital, in the period between Mars 2018 and December 2019. This is a case series study of 37 patients upon whom Hayman suture is applied when failed to respond to medical management, step-wise devascularization of the uterus is attempted when the suture cannot control the bleeding alone, reserving hysterectomy as a last resort when all measures are failed. Results: Results of the research None of the 37 patients of our study required hysterectomy to control the bleeding, Hayman suture succeeded solely to arrest the hemorrhage in 30 patients (81.1%), while 7 patients(18.9%) required step-wise devascularization of the uterus with Hayman suture to stop the bleeding. It is not uncommon for complications to be described with the use of uterine compression sutures. In our study, we did not report any complications. Conclusion: The application of Hayman’s suture is a fast, efficient, non-complex, lifesaving and fertility-sparing technique to control atonic postpartum hemorrhage.
Hawar M.H. Zebari
Journal of Scientific Research in Medical and Biological Sciences, Volume 2, pp 30-37; https://doi.org/10.47631/jsrmbs.v2i4.332

Abstract:
Purpose: The objective of the present study was to compare the reproductive performance in synchronized and spontaneous estrus sheep raised under farm conditions. Subjects and Method: Two hundred Karadi and Two hundred Awassi were used in this study. Of each breed, 100 ewes were allowed to come in estrus spontaneously and the other 100 ewes were synchronized using intravaginal sponges (ESPONJAVET, HIPRA, Amer, Girona, SPAIN). Ewes were injected with intramuscular injection of 500 IU of PMSG when sponges were removed on day 14. Results: This study revealed that estrus synchronization had a higher rate of lambing (P=0.014), twining (P<0.001), dystocia (P=0.024), and barrenness (P=0.012) in Karadi ewes. Estrus synchronization also had a significantly higher rate of lambing (P=0.018), twining (P=0.017), abortion (P<0.001), barrenness (P=0.012) and productivity (P=0.010) in Awassi ewes. the result of the current study shows that the barrenness rate was higher in spontaneously ovulated ewes compared to synchronized Awassi ewes. The result of the current study shows that the abortion rate was higher in spontaneous ewes compared to synchronized ewes. The twinning rate was higher in synchronized compared to spontaneous Karadi ewes. Conclusion: In both breeds, synchronized ewes had higher reproductive efficiency compared to ewes that had spontaneous estrus.
Ibrahim Arafa I. Hamed, Nahla Waer Shady, Abdu Saeed Ait-Allah
Journal of Scientific Research in Medical and Biological Sciences, Volume 2, pp 57-63; https://doi.org/10.47631/jsrmbs.v2i4.355

Abstract:
Purpose: The study examines how useful laparoscopy is in finalizing the diagnosis of unexplained infertility. Materials: The study included 50 women with 1 year or 2 years of infertility, who had a regular marital life with unprotected intercourse for more than a year, normal husband's semen analysis, normal ovulation by folliculometry, normal hormonal profile, and normal hysterosalpingogram at the department of Obstetrics and Gynecology at Aswan University Hospital from January to December 2019. Methods: This is a cross-sectional observational study involving 50 women who underwent diagnostic laparoscopy after investigations and imaging failed to reveal the cause of infertility. During the procedure, the pelvis was inspected for any pathology, including the uterus, fallopian tubes, round ligaments, ureterovesical pouch, uterosacral ligaments, Douglas pouch, and ovarian fossae. Results: Diagnostic laparoscopy helped uncover abnormal pathologies such as endometriosis, adhesions, and tubal pathologies in 30 women (60%) of the 50 included in the study, whereas no abnormality was detected in the remaining 20 (40%). Conclusion: Laparoscopy is not only a crucial diagnostic technique in infertility patients, but it can also help with treatment selections. Conducting laparoscopy in cases of unexplained infertility is linked to both peritubular adhesions and pelvic endometriosis. These pelvic disorders might not be appropriately detected or treated without laparoscopy, and hysterosalpingography and basic imaging such as pelvic ultrasonography are frequently ignored.
Mohammad Abdelmonaem Sharaf, Heba Ezzat Hashem, Wafaa O. Ahmed
Journal of Scientific Research in Medical and Biological Sciences, Volume 2, pp 1-10; https://doi.org/10.47631/jsrmbs.v2i4.346

Abstract:
Purpose: The study examined the use of factor XIII and fibrin degradation products in diagnosing early cases of NEC and neonatal sepsis. Methods: Sixty neonates were divided into two groups. 30 preterm neonates suspected with early NEC Diagnosis of NEC was confirmed by modified Bell’s score and 30 preterm neonates with symptoms of neonatal sepsis; where sepsis was confirmed by blood culture and CRP. Laboratory evaluation of FDPs and plasma factor XIII was done for all the patients. The study was carried out in a tertiary NICU of the pediatric department, Ain Shams University Hospital. All enrolled neonates had a matched mean birth weight and gestational age. They were either moderate preterms >32 weeks, but 34 weeks, but <37 weeks). Results: The results indicate a correlation between FDPs and the laboratory data of group B, and it was found out that FDPs were negatively correlated with TLC, Plate-lets, and CRP, reflecting FDPs increase with bone marrow suppression and progression of sepsis. Factor XIII was significantly lower in the group with NEC as compared to the group of sepsis (p<0.001), while FDPs level was significantly higher in the group with sepsis (p<. 0.001). The correlation between the clinical stages of NEC BELL's score and the level of Initial factor XIII level revealed that the factor level is negatively correlated with stage I of BELL's score. The follow-up revealed that there was no correlation between BELL's score and the level of follow-up factor XIII. On follow-up, the current study demonstrated that TLC, CRP, FDPS, PTT were significantly increased in the sepsis group with p values of 0.021,, 0.001, 0.001 and 0.01. The current study found significantly higher partial thromboplastin time (PTT) in the group with sepsis Conclusion: Factor XIII level can predict early cases of NEC and can differentiate it from neo-natal sepsis.
Manju Lata, Mohammad Jamali
Journal of Scientific Research in Medical and Biological Sciences, Volume 2, pp 44-56; https://doi.org/10.47631/jsrmbs.v2i4.337

Abstract:
Purpose: This study aimed to document the present status of medicinal plants used to boost the immunity to combat Coronavirus. Materials and Methods: For the assessment and use of herbs during the Covid 19 pandemic, surveys were conducted in different villages of Seraj block. The surveys were conducted during the spread of the pandemic from 2020 to 2021. Local people were interviewed and information on the utilization of local herbs during this pandemic was recorded. Information on parts used, the procedure of use, habit, habitat, etc. was also collected. Results: A total of 58 species of medicinal plants from 27 families and 42 genera were documented. Among them, the most dominant family was Rosaceae. Most of the plants such as Morchella sp, Naustratium officinale,Taraxacum officinale ,Urtica dioica ,Allium sp,Withania foraminifera, Curcuma longa, Cannabis, Mentha, Ocimum sanctum are rich in antioxidants and have a wide range of medicinal values used to treat cough, cold, fever, and bronchitis. All these plants were used traditionally to boost immunity. Due to the covid -19 pandemic, the utilization of these herbal plants has increased many folds in the area. These medicinal plants should be made available to scientists to design clinical trials. Integration of this concept would certainly develop drug therapy in the near future. Conclusion: The study found that the use of medicinal plants has increased during COVID-19 and most of the respondents recommended medicinal plants to prevent COVID-19 and to boost immunity. More studies should be conducted to develop certain formulations.
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