Molecular Microbiology

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ISSN / EISSN : 0950-382X / 1365-2958
Current Publisher: Wiley (10.1111)
Former Publisher:
Total articles ≅ 15,604
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Latest articles in this journal

Ying‐Jung Lai, Helen Yakhnin, Archana Pannuri, Christine Pourciau, Paul Babitzke,
Published: 9 June 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14769

The publisher has not yet granted permission to display this abstract.
Ankita J. Sachla, Yuanchan Luo,
Published: 7 June 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14767

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Emily A. Masser, Peter E. Burby, Wayne D. Hawkins, Brooke R. Gustafson, Justin S. Lenhart,
Published: 7 June 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14765

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Ambika Dattani, Isatou Drammeh, Aishah Mahmood, Mahbubur Rahman, Joanna Szular,
Published: 1 June 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14763

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Mohammad Sadik, Mohammad Afsar, Ravishankar Ramachandran,
Published: 25 May 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14735

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Xuejiao Song, Sarah K. Henke,
Published: 24 May 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14761

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Christopher I. Graham, Palak G. Patel, Jennifer R. Tanner, Jacqueline Hellinga, Teassa L. MacMartin, ,
Published: 20 May 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14760

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Ran Zhang, Mac A. Shebes, Kelvin Kho, Salvatore J. Scaffidi, ,
Published: 4 May 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14734

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Lauren V. Carruthers, Jane C. Munday, Godwin U. Ebiloma, Pieter Steketee, Siddharth Jayaraman, , Marzuq A. Ungogo, Leandro Lemgruber, Anne‐Marie Donachie, Tim G. Rowan, et al.
Published: 1 May 2021
by Wiley
Molecular Microbiology; doi:10.1111/mmi.14733

Abstract:
Trypanosoma congolense is a principal agent causing livestock trypanosomiasis in Africa, costing developing economies billions of dollars and undermining food security. Only the diamidine diminazene and the phenanthridine isometamidium are regularly used, and resistance is widespread but poorly understood. We induced stable diminazene resistance in T. congolense strain IL3000 in vitro. There was no cross‐resistance with the phenanthridine drugs, melaminophenyl arsenicals, oxaborole trypanocides, or with diamidine trypanocides, except the close analogues DB829 and DB75. Fluorescence microscopy showed that accumulation of DB75 was inhibited by folate. Uptake of [3H]‐diminazene was slow, low affinity and partly but reciprocally inhibited by folate and by competing diamidines. Expression of T. congolense folate transporters in diminazene‐resistant T. b. brucei significantly sensitized the cells to diminazene and DB829, but not to oxaborole AN7973. However, [3H]‐diminazene transport studies, whole genome sequencing and RNA‐seq found no major changes in diminazene uptake, folate transporter sequence or expression. Instead, all resistant clones displayed a moderate reduction in the mitochondrial membrane potential. We conclude that diminazene uptake in T. congolense proceed via multiple low affinity mechanisms including folate transporters; while resistance is associated with a reduction in Ψm it is unclear whether this is the primary cause of the resistance.
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