Sučasne ptahìvnictvo

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ISSN / EISSN : 2309-6659 / 2415-7686
Total articles ≅ 28
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Y.Y. Dovbnia, National University of Life and Environmental Sciences of Ukraine, L.V. Shevchenko, Т.B. Zheltonozhskaya, S.V. Shulyak, Institute of Macromolecular Chemistry, State Research Institute of Laboratory Diagnostics and Veterinary Sanitary Examination
Sučasne ptahìvnictvo pp 25-29; https://doi.org/10.31548/poultry2021.07-08.025

Abstract:
Nanosilver preparation is synthesized in the carriers based on polymer/inorganic hybrids in aqueous dispersion form by in situ synthesis of silver nanoparticles in biocompatible and biodegradable polymer/inorganic hybrid based on silica sol and polyacrylamide. Hydrophilic polymer/inorganic hybrid used as a carrier for silver nanoparticles was synthesized by the developed method of directly grafting polyacrylamide "from" the unmodified surface of silica sol. The size of silver particles in the preparation was <10 nm. Studies have found that single, double and three-fold sprinkling of laying hens of nanosilver in polymer/inorganic hybrid carriers at concentrations of 1.0 and 2.0 mg/l (at doses of 0.2 and 0.4 mg per hen per day) did not affect the consumption of feed, water and egg productivity of poultry. The obtained nanosilver preparation is safe for laying hens and it does not cause disorders of clinical condition, diseases and poultry death during three-fold drinking with 10 days interval. After drinking the preparation of nanosilver to the laying hens at doses of 0.2 and 0.4 mg per head per day with an interval of 10 days, the dose-dependent amount of silver in the manure was increased only after a single drinking and after double and triple drinking, it did not affect the content of silver, copper, zinc, iron and lead. Single drinking of laying hens with a solution of silver nanoparticles in carriers based on polymer/hybrids at a concentration of 1.0 mg/l (0.2 mg per hen per day) increased the silver content in hen manure by 20% compared to the control group, and it did not affect the content of copper, zinc, iron and lead in manure. Nanosilver preparation at a concentration of 2.0 mg/l (0.4 mg per hen per day) increased the silver content by 44% in hen manure on the 10th day only after the first drinking of the preparation and it did not affect the content of copper, zinc, iron and lead compared to the control and with similar data of hens to which the same preparation was given at a concentration of 1.0 mg/l.
O.V. Tsinoviy, State Research Station of Poultry NAAS, G.V. Biletska
Sučasne ptahìvnictvo pp 18-24; https://doi.org/10.31548/poultry2021.07-08.018

Abstract:
A new method of purification and concentration of goose enteritis virus has been developed. For this purpose, three options for further purification of the virus were used and their comparative analysis was performed. The virus was purified without detergents, as well as with detergents – sarcosyl and nonide R-40. We obtained the best results using the mild nonionic detergent nonidet R-40, which was used in our further work. The virus was identified by electrophoretic studies in polyacrylamide gel, as well as electron microscopy. During purification and concentration of the virus, the infectious titer of the virus was 9.2-9.5 lg TCD50/cm3 suspension, in which the cell monolayer in 50% of the wells was affected by cytopathic action), which is 2 lg TCD50/cm3 higher than in the original material. The protein content in the test samples ranged from 200 to 500 mg/ ml. Thus, analyzing our results on the purification and concentration of goose enteritis virus, we can conclude that the antigen obtained by this method is suitable for the development of enzyme-linked immunosorbent assay (ELISA). Hyperimmune and negative sera for ELISA diagnostics were obtained, the optimal ratios of components for designing an ELISA test system were worked out, and the formula for recalculating antibody titers in geese blood sera when testing them in one dilution was derived. A positive-negative threshold was determined for this diagnosticum (which of the studied sera have a positive, doubtful or negative titer of antibodies to the causative agent of viral enteritis in geese). In the new conditions the spread of particularly dangerous viruses, this development, with the appropriate equipment, can be further used to purify and concentrate these viruses, study their biological properties, cultivate and use them in the development of new vaccines.
V. Honchar, National University of Life and Environmental Sciences of Ukraine, O. Iakubchak
Sučasne ptahìvnictvo pp 7-13; https://doi.org/10.31548/poultry2021.07-08.007

Abstract:
The effect of feeding lycopene and astaxanthin to laying hens on the morphological parameters of edible eggs was investigated. The experiment consisted of three periods and lasted 90 days. For this, 3 groups of chickens were formed, 15 heads each. The control group was fed complete feed, the first for its research group - lycopene at doses of 20, 40, 60 mg/kg of compound feed, the second research group-astaxanthin at doses of 10, 20, 30 mg/kg of compound feed from 1 to 30, from 31 to 60, from 61 for 90 days, respectively. Eggs were taken from each group of laying hens and divided into two lots. The first batch was stored at 4±0.5 °C, and the second at 12±0.5°C. Both batches of eggs were stored for 30 days. Feeding hens-laying hens with lycopene at a dose of 20, 40, 60 mg/kg or astaxanthin at a dose of 10, 20 and 30 mg/kg of compound feed does not affect the morphological parameters of eggs when stored at 4±0.5 °C. The addition of lycopene at a dose of 20 mg/kg or astaxanthin at a dose of 10 mg/kg of compound feed, at a temperature of 12±0.5 °C for 30 days, contributed to a decrease in the weight of chicken eggs by 0.66 and 0.92%, respectively, which happened by reducing the mass of protein by 1.01 and 1.73%, respectively. Storage of eggs for 30 days at a temperature of 12±0.5 °C obtained from chickens fed with supplements of both lycopene at a dose of 40 mg/kg and astaxanthin at a dose of 20 mg/kg of compound feed contributed to a decrease in egg weight by 0.75% and the mass of protein-by 1.13%, respectively, and by 0.78% – the mass of eggs and the mass of protein – by 1.02%, respectively. Storage of eggs from chickens that received supplements of lycopene at a dose of 60 mg/kg or astaxanthin at a dose of 30 mg/kg of compound feed at a temperature of 12±0.5 °C led to a decrease in the weight of eggs by 0.71 and 0.67%, respectively, and the weight of protein by 1.19 and 1.56%, respectively.
I.M. Grechkivsky, National University of Life and Environmental Sciences of Ukraine, M.Ya. Kryvenok, V.M. Mykhalska
Sučasne ptahìvnictvo pp 11-15; https://doi.org/10.31548/poultry2021.05-06.011

Abstract:
It is established that the growth of young quails depends on the optimal glycine content in the feed. The article presents data on the use of compound feed with different levels of glycine in the feeding of young quails of meat productivity. The research was conducted in the vivarium of the Department of Animal Feeding and Feed Technology. PD Wheat on quail breed Pharaoh. 300 day-old quails were selected for the experiment and three groups were formed. The level of glycine in feed for experimental animals was regulated by the introduction into its composition of a synthetic preparation of this amino acid by weight dosing and stepwise mixing. It was found that the live weight of quails depended on the glycine content in their diet. Thus, from the age of 14 days, the quails of the second and third groups had a live weight higher by 1.2% and 1%, respectively, compared to the control. At 21 days of age, the live weight of quails of the second group, which was fed feed containing 1.50% glycine, was 0.9% higher than in the control group, and the third group, with the level of glycine 1.60% – 1% lower. When feeding quail feed with a content of 1.50% glycine, their live weight at 28 days of age was 1.5% (P<0.01) greater than the control, and the live weight of birds of the third group was 1.5% (P <0.01) less. At the age of 35 days, the quails of the second group had the largest live weight, which is 0.9% more than the birds of the control group. During the first week of life, quails of the second group had an average daily gain less than analogues from the control group by 1.9%; and a week later, this figure increased by 2.6% compared to control. The highest average daily gain for the entire period of the experiment was in poultry of the second group – 8.72 g, which is 0.9% higher than in the control. Thus, it was found that feeding quails compound feed with a glycine content of 1.50% contributes to an increase in their live weight by 0.94%, average daily gains – by 0.9%, and reducing feed costs per 1 kg of growth by 1.4%.
O.V. Tsinoviy, State Research Station of Poultry NAAS, L.I. Nalyvayko
Sučasne ptahìvnictvo pp 24-31; https://doi.org/10.31548/poultry2021.05-06.024

Abstract:
In the absence of diagnostic kits for the detection of antibodies to metapneumovirus infection (MPVI) epizootological monitoring in Ukrainian farms is practically not carried out, imported test systems have a "sky-high" price, so there is a need for domestic methods of diagnosing this disease. The most accurate, easy-to-use method is ELISA-based test systems (enzyme-linked immunosorbent assay). A diagnostic ELISA test system for the detection of antibodies to MPVI has been developed and it has been established that this diagnosticum should be used in the practice of veterinary medicine for serological control of metaviral virus infection. The optimal ratios of components for the manufacture of ELISA test system have been worked out. The form of calculation of antibody titers in blood sera of chickens when testing them in one dilution is calculated. The sensitivity and specificity of the ELISA test system were determined (comparative analysis of serum testing results in ELISA, RNGA and RN). Scientific documentation has been developed – instructions for the manufacture and control of ELISA test systems for the detection of antibodies to metapneumovirus infection in the serum of chickens and instructions for its use. Indication and identification of the obtained virus isolate was carried out using polymerase chain reaction (PCR) and nucleotide sequencing. Based on the studies carried out in the suspension of the internal organs of turkeys (trachea, lungs), a virus belonging to subtype B of the genus Metapneumovirus, subfamily Pneumovirinae, family Paramyxoviridae of the order Mononegavirales was revealed. By comparing the nucleotide sequence of the G gene fragment of the PVT-09/B strain with the sequences of strains and isolates of the avian metapneumovirus subtype B published in the GenBank database, it was found that the metapneumovirus isolated from sick turkeys is phylogenetically close to the Brazilian strains 27A-07 2007 and MPV/B/Brazil-07/USP-08 G
V.A. Davidovych, National University of Life and Environmental Sciences of Ukraine, L.V. Shevchenko, V.M. Mykhalska
Sučasne ptahìvnictvo pp 19-23; https://doi.org/10.31548/poultry2021.05-06.019

Abstract:
The introduction of feed for poultry carotenoids that are not transformed in the body, including lycopene and astaxanthin, causes an attractive commercial appearance of chicken egg yolks. The production of high-quality and safe table eggs involves limiting the use of synthetic yolk dyes and switching to natural dyes, including tomato and seaweed products. The aim of this study was to determine the egg productivity of poultry, morphological composition of eggs, as well as feed and water intake with the addition of oil extracts of lycopene (20, 40 and 60 mg/kg or feed) or astaxanthin (10, 20 and 30 mg/kg of feed) to the diet. laying hens. The experiment was performed on 45 chickens of the High Line W36 cross at the age of 24 weeks. Each additive was fed to laying hens for 30 days in increasing concentrations. The use of lycopene or astaxanthin in different doses to laying hens did not have a negative impact on the clinical condition of the bird and behavior, did not cause death of birds of the experimental groups throughout the experiment. The condition of feathers and visible mucous membranes in chickens during the use of experimental diets was characteristic of clinically healthy birds. It was found that the addition of lycopene at doses of 20, 40 and 60 mg/kg and astaxanthin at doses of 10, 20 and 30 mg/kg of compound feed for 30 days did not significantly affect feed consumption and egg productivity. Increasing the content of astaxanthin to 20 and 30 mg/kg of compound feed, as well as lycopene to 60 mg/kg of compound feed resulted in a slight decrease in water consumption by laying hens. Feeding laying hens supplements of astaxanthin oil extract at doses of 10, 20 and 30 mg/kg or lycopene at doses of 20, 40 and 60 mg/kg of compound feed for 90 days did not significantly affect the weight of eggs, as well as the weight of protein, yolk and shell. Additions of lycopene and astaxanthin oil extracts to the diet of laying hens may be promising for use in correcting the color of egg yolks.
A.M. Fedorchenko, Bila Tserkva National Agrarian University, V. V. Nedosekov, National University of Life and Environmental Sciences of Ukraine
Sučasne ptahìvnictvo pp 22-27; https://doi.org/10.31548/poultry2021.03-04.022

Abstract:
The movement to ensure Ukraine's integration into the EU is a modern direction of the current state reform. A positive reflection of this process is the official adoption of a number of legislative documents in accordance with international requirements, the standards of which regulate the production of safe and quality products, including poultry. Thus, the implementation of the necessary HACCP (Hazard Analysis and Critical Control Point) requirements for product safety and quality at the level of European standards was able to introduce in the country only a small number of large enterprises in the poultry industry. Most medium and small poultry farms in Ukraine have not implemented the requirements of the HACCP standard and international ISO quality standards, which limited their ability to control product safety in accordance with international requirements and became an obstacle to selling their products in foreign markets. A positive and necessary point of implementation of the European biosafety requirements of the HACCP principles is the expanded possibility in carrying out effective and detailed control of safety and quality indicators of food products in the poultry industry. This control should be established by clearly defined components that are interconnected in interconnected technological processes. Such components in poultry hatcheries are: a detailed analysis of critical control points of hazards of each stage of the production process; use of components and raw materials in general; application of timely monitoring, preventive anti-epizootic and corrective measures to prevent danger at all production sites of poultry hatcheries. Thanks to the constant control of all critical control points of dangers in poultry hatcheries, it is possible to achieve the production of safe and high-quality products obtained from healthy poultry in the poultry industry.
O.V. Tsinoviy, National University of Life and Environmental Sciences of Ukraine, L.I. Nalyvayko
Sučasne ptahìvnictvo pp 9-14; https://doi.org/10.31548/poultry2021.03-04.009

Abstract:
The main biological (cultural) properties of field isolates of metapneumovirus (MPV) were studied, normal and hyperimmune sera to metapneumovirus for IHR-diagnosticum (erythrocyte diagnosticum based on indirect hemagglutination reaction) were obtained, and a set of M-component components was developed. A diagnostic system of IHR (indirect hemagglutination reaction) was developed, with the help of which epizootological monitoring was carried out in poultry farms and the spread of a new infectious disease of poultry (metapneumovirus infection) among turkeys and chickens in poultry farms of Ukraine was studied. The purpose of the research is to develop a domestic diagnostic test system (erythrocyte diagnosticum for IHR) for metapneumovirus infection (MPVI) or infectious avian rhinotracheitis (IRT). As a result of the conducted researches metapneumovirus infection or infectious rhinotracheitis was established in poultry farms of 4 regions of Ukraine. The pathogen was isolated, its molecular-biological properties were studied by PCR (polymerase chain reaction), and it was established that it belongs to the genus Metapneumovirus (MPV), subtype B. The results of the epidemiological monitoring indicate that the developed erythrocyte MPV (IRT) antigen based on IHR is sensitive and specific and can be used to control the spread of metapneumovirus infection and the intensity of immunity in birds vaccinated against this disease. The production inspection in poultry farms in the western regions of Ukraine established the possibility of using IHR-diagnosticum for control of MPVI. As a result of the performed work the new domestic method of diagnostics, forecasting and protection of poultry against a metapneumovirus infection is offered. Prospects for further research are to use this erythrocyte diagnosticum based on the indirect hemagglutination reaction to monitor metapneumovirus infection of birds in poultry farms in Ukraine and determine the epizootic situation for this disease.
V.M. Poliakovskyi, National University of Life and Environmental Sciences of Ukraine, V.M. Mykhalska, L.V. Shevchenko
Sučasne ptahìvnictvo pp 6-10; https://doi.org/10.31548/poultry2021.01-02.006

Abstract:
This article discloses the requirements for the installation of poultry houses and indoor equipment for keeping guinea fowl. In the construction of poultry houses it is necessary to take into account the behavioral and physiological characteristics of guinea fowl on which their growth and development, productivity, feed consumption and disease resistance. For construction of a poultry house choose the elevated site or with a small slope that thaws and rain waters did not collect and did not stagnate. The size of the poultry house depends on the number of livestock in it, so it is recommended to place up to 5 heads per 1 m2 of floor area. The roof of the poultry house is made sloping. Litter is used in the premises for keeping guinea fowl: peat, straw, sawdust, litter thickness –10-15 cm. During the whole winter period the litter is not removed, only fresh is added regularly. If the litter is heavily soiled, the contaminated part is removed and a clean one is added. For winter keeping of guinea fowl the room is well warmed, without allowing in it humidity and formation of a mold. When keeping guinea fowl in the summer, it is recommended to equip them with a walking yard, fenced with wire mesh, around which bushes and trees are planted or canopies are made to protect the bird from direct sunlight. In winter, an artificial heat source is installed in the poultry house, for example, electric brooders, electric lamps with a metal shade-reflector located at a height of 15-20 cm from the floor. Seats for guinea fowl are made of chipped bars with rounded upper edges, which are installed on the opposite side of the windows in a horizontal form. Nests are set in the henhouse long before the laying hens begin to lay eggs, so that the guinea fowl have time to get used to them and lay eggs in the nests. Feeders are made like troughs, which prevents contamination and scattering of food. A turntable is mounted on the brackets on top of the feeder, it rotates around its axis and also prevents the feed from scattering. At the height of the feeder is made so that the edges of the sides were at the level of the back of the bird. It is better to use vacuum or nipple drinkers to water the guinea fowl, which provide them with fresh running water.
I.M. Cheverda, National University of Life and Environmental Sciences of Ukraine, M.о. Zakharenko, V.V. Solomon
Sučasne ptahìvnictvo pp 18-23; https://doi.org/10.31548/poultry2021.01-02.018

Abstract:
The effect of gonadoectomy (removal of the testicles) in Adler's silver roosters on the clinical condition, live weight, slaughter rates and chemical composition of the pectoral muscle was studied. The experiment was performed on 40 roosters of 6-weeks, which according to the principle of analogues were divided into two groups: control and experimental 20 heads each. It was found that in the preparatory period, which lasted 10 days indicators of the clinical condition – body temperature, pulse and respiration, as well as live weight of the roosters of the experimental group did not differ from the control. Gonadoectomy of roosters of the experimental group, conducted at the age of 45 days by a specially developed method, increased the body temperature of the poultry on the second day after surgical intervention by 1.03°C, and on the third – by 1.15°, while other indicators of clinical condition – pulse, number respiratory movements and live weight did not change compared to control. The duration of the postoperative period in the roosters of the experimental group was 3-4 days and ended with the healing of the wound on the skin. Subsequently, body temperature, pulse and the number of respiratory movements in gonadoectomized roosters corresponded to similar indicators of poultry in the control group. The live weight of gonadoectomized roosters in the rearing process, which was monitored decadelly from the 65th to the 95th day compared to the control did not change, on 105th and 115th day had a tendency to increase, and 125th day increased by 4.8 %. Slaughter rates of gonadoectomized roosters corresponded to similar indicators of poultry of the control group. The weight of the gutted carcass was higher by 5.2% and that of the muscular stomach by 7.8%. Differences in the chemical composition of the pectoral muscle of gonadoectomized roosters compared with control, namely: on the content of dry matter, moisture, fat, protein and ash were not found. Thus, gonadoectomy of roosters of the Adler's silver meat-egg breed does not affect their clinical condition, the chemical composition of the pectoral muscles, but increases the live weight of the bird on the 125th day of rearing
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