Journal of Stem Cell Research and Tissue Engineering

Journal Information
ISSN / EISSN : 2614-1264 / 2614-1256
Published by: Universitas Airlangga (10.20473)
Total articles ≅ 45
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Journal of Stem Cell Research and Tissue Engineering, Volume 6, pp 15-24; https://doi.org/10.20473/jscrte.v6i1.37512

Abstract:
Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, first is stem cells that have self renewal ability and multineage differentiation, second is bioreactor / growth factor, and then scaffold. The combination of hADMSC and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Purpose to prove osteoinduction in a combination of Human Adiposed Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold using blood serum calcium levels. Methods: This study uses 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at 1st,3rd,7th, and 14th days which maxillary bone drilling only. While groups 5 to 8 were the positive control group at 1st,3rd,7th, and 14th days which were given chitosan scaffold. Groups 9 to 12 were treatment group at 1st,3rd,7th, and 14th days which were given hADMSC and chitosan scaffold. Blood collection is carried out in each group to check serum calcium levels. Result there were differences in calcium levels in blood serum in each group. Conclusion the application of hADMSC and chitosan scaffold caused a significant change in serum calcium levels on the 1st, 3rd, 7th and 14th days which meant that the combination of hADMSC and chitosan scaffold could trigger osteoinduction.
Journal of Stem Cell Research and Tissue Engineering, Volume 6, pp 25-31; https://doi.org/10.20473/jscrte.v6i1.37515

Abstract:
The role of type I collagen is as a matrix of extracellular proteins with characteristics of increased cell proliferation which directly affects the physiology and morphology of cells. Type 1 collagen can be obtained either from fish scales. This is what underlies the author to support engineering tissue used for the treatment of periodontal disease in the regenerative field by utilizing collagen derived from gouramy scales. As an initial step, the researchers wanted to conduct a study using collagen extract derived from gouramy scales (Osphoronemus gouramy) which was applied to bone marrow mesenchymal stem cell cultures to see viability in vitro. To determine the viability of collagen in carp (Osphronemus goramy) scales to bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells are taken from mice and planted in 96 well plates. Collagen extracted from gouramy scales using the enzymatic method was dissolved in a condition medium and hydrolyzed into a collagen hydrolysis solution with each concentration of 0.01 mg / ml, 0.02 mg / ml, 0.04 mg / ml, 0.16 mg / ml, 0.32 mg / ml was put into the well prepared and incubated for 24 hours for the MTT assay. Collagen in carp scales can increase the viability of bone marrow mesenchymal stem cells with a percentage above 50% and the highest viability concentration at 0,01 mg / ml. The collagen of gouramy scales soaked in a medium condition has better viability than the collagen hydrolysis solution of carp. Collagen in carp scales is viable against bone marrow mesenchymal stem cells. Collagen scales of gouramy soaked in medium had the highest viability with an optimum dose of 0.01 mg / ml.
Journal of Stem Cell Research and Tissue Engineering, Volume 6, pp 32-38; https://doi.org/10.20473/jscrte.v6i1.37516

Abstract:
Periodontal disease is a pathological inflammatory condition of the periodontal tissues surrounding the teeth, including Human Gingival Fibroblasts (HGF) which is one of the major components of tissue formation in periodonsium. HGF regeneration with the accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering using regenerative materials from cow or pig as the therapies, but these materials have some flaws so this research to find alternative materials regenerative tissue engineering scaffold collagen type 1 derived from fresh water fish scales, one of which are gourami fish scales. This research was conducted to test the viability of fish scales collagen gouramy against Human Gingival Fibroblasts for 24 hours. This study aimed to determine the concentration of fish scale collagen gourami which can maintain the viability of human gingival fibroblast cells for 24 hours. HGF is taken from healthy gingiva and planted in 96 well plates. Fish scales collagen gouramy with a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added to each well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells. The viability of HGF were increased after the addition of fish scales collagen gourami on concentration 0.32 mg/ml until 0.01 mg/ml. The highest viability of the cells was shown after the addition of 0.01 mg/ml. Fish scales collagen gouramy has the potential in tissue engeneering and the concentration of 0.01 mg/ml shows the highest viability of HGF.
Journal of Stem Cell Research and Tissue Engineering, Volume 6, pp 39-47; https://doi.org/10.20473/jscrte.v6i1.37514

Abstract:
Bone defect is one of the challenges for dentists in the process of healing bone tissue. Bone defect can occur in alveolar bone with the etiology of microorganisms and cyst expansion. In addition, cases of bone defects in alveolar bone are also often found in cases with treatment of apex resection and hemisection. Autologous bone graft is a clinical gold standard in the treatment of bone defect. However, the use of bone graft has a limited number of growth factors produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components; stem cell, growth factor, and scaffold. Stem cells will increase osteoblastogenesis and chitosan scaffold will immobilize alkaline phosphatase (ALP) so that serum ALP levels decrease and bone regeneration and mineralization processes become faster. The aim of this study is analyzing the effect of human adipose-derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) in bone defect on serum alkaline phosphatase (ALP) levels. This research was a in vivo laboratory experimental study. Bone defects are planted with chitosan scaffold (CS) and a combination of human adipose-derived mesenchymal stem cells (HADMSC) with chitosan scaffold. Measurement of ALP levels was carried out by the International Federation of Clinical Chemistry (IFCC) method using an analyzer on the 1st, 3rd, 7th and 14th days. Research data were analyzed using multivariate analysis of variance (MANOVA) and Bonferroni tests. The results of the data analysis showed that there were significant differences in ALP levels with CS planting and the combination of HADMSC and CS. the effect of human adipose- derived mesenchymal stem cell (HADMSC) with chitosan scaffold (CS) on bone defect reduces serum alkaline phosphatase (ALP) levels on the 3th and 14th days.
Journal of Stem Cell Research and Tissue Engineering, Volume 6, pp 1-14; https://doi.org/10.20473/jscrte.v6i1.37511

Abstract:
Cervical cancer is a disease caused by a malignant process that occurs in the cervix or cervix. The cause of cervical cancer is not known for certain, but it is estimated that around 95% is caused by HPV (Human Papilloma Virus). Efforts to cure cancer with drugs (pharmacotherapy) or with chemical compounds (chemotherapy) in general have not been able to give satisfactory results, so alternative treatment methods are sought, including traditional medicine, namely by using mangroves. Lumnitzera racemosa is one type of mangrove plant that has been used in alternative medicine because of its potential as anticancer. The aim of this study was to determine the effect of Lumnitzera racemosa mangrove extract on hela cell viability. Lumnitzera racemosa leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 56 ppm, it means that the ethanol extract has toxic properties. The results of the phytochemical test of the leaf extract of Lumnitzera racemosa contained alkaloids, steroids, triterpenoids and saponins. The test results showed that the extract yield was 11.58%, the water content of the extract was 22.17%, and the total phenol was 2742.17 mg GAE. The test results from the LC-MS test resulted in suspected compounds including pyrogallol, isoniazid and caffeine. The ethanolic extract of Lumnitzera racemosa leaf was cytotoxic to the viability of hela cells with the resulting IC50 value of 493.33 µg/mL.
Nindya Rizqi Anjani
Journal of Stem Cell Research and Tissue Engineering, Volume 5, pp 72-79; https://doi.org/10.20473/jscrte.v5i2.33146

Abstract:
Reconstruction of extensive bone tissue damage is a treatment with complication. Because moving the autologous tissue such as bone graft can cause complications that causes problems in the repair of extensive tissue damage so, the principle of tissue engineering (stem cells, bioreactor / growth factor, and scaffold) is used as an alternative to reconstruct damage to the tissue because it has many advantages. The combination of hADMSC and chitosan scaffold, is expected to trigger osteoinduction that can be expressed by osteogenic markers such as phosphorus levels in blood serum. To prove osteoinduction in a combination of Human Adiposed Derived Mesenchymal Stem Cell (hADMSC) and chitosan scaffold using blood serum phosphorus levels. This study used 12 groups with 5 sample each. Groups 1 to 4 were the negative control group at day 1,3,7, and 14. While groups 5 to 8 were the positive control group at day 1,3,7, and 14. Groups 9 to 12 were treatment groups at day 1,3,7, and 14. In the negative control group bone was only removed, in positive control group, bone was removed and chitosan scaffold was added, and in treatment group, bone was removed then, hADMSC and chitosan scaffold combination was added . Blood collection will be carried out in each group for examination of phosphorus levels in the blood serum. There were differences in phosphorus levels in blood serum in each group even though statistically there were only significant differences on day 14. The combination of hADMSC and chitosan scaffold caused a significant change in blood serum phosphorus levels on day 14 which means it triggers osteoinduction.
Ully Nafisah Wardi
Journal of Stem Cell Research and Tissue Engineering, Volume 5, pp 87-102; https://doi.org/10.20473/jscrte.v5i2.33147

Abstract:
Inflammation and alveolar bone resorption are indications of periodontal disease, which is a chronic inflammatory illness caused by bacterial colonization that damages the soft and hard structures that support the teeth. In response to persistent tissue injury and chronic inflammation, fibroblasts also play a role in the synthesis and maintenance of extracellular matrix, cell proliferation, and cell differentiation. Fibroblasts play a crucial part in the healing of wounds. Phenols, alkaloids, flavonoids, and tannins are some of the health-promoting components found in water hyacinth. As a result, plant extracts must be tested first, one of which is the viability test in accordance with the requirements and materials in the field of dentistry. The viability test is a cell-based test that is often used for screening compounds to determine whether the test compound has an effect on cell proliferation or has a direct cytotoxic effect that leads to cell death. The goal of this study is to figure out what concentration of water hyacinth leaf extract can keep human gingival fibroblast cells alive for 24 hours. Primary cell cultures from human gingiva were extracted and placed in a 96-well microplate. For 24 hours, water hyacinth leaf extract at concentrations of 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml, 0.25 mg/ml, 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml, 0.0156 mg/ml was administered to each well in the microplate. After 24 hours of incubation, the MTT assay was carried out by adding MTT solution. The optical density of formazan was measured using an ELISA reader at a wavelength of 590 nm, and viability was calculated using the viability formula. Starting at 0.125 mg/ml, 0.0625 mg/ml, 0.0312 mg/ml, and 0.0156 mg/ml, the vitality of human gingival fibroblast cells was good. In the treatment group, the greatest vitality of human gingival fibroblast cells was 0.0156 mg/ml (75.98%).
Ikhram Kharis
Journal of Stem Cell Research and Tissue Engineering, Volume 5, pp 61-71; https://doi.org/10.20473/jscrte.v5i2.33145

Abstract:
The use of biomaterial such as bone graft material is highly needed in oral and maxillofacial surgery to overcome bone defect that happened due to various reasons. One of the bone graft that widely used is bovine hydroxyapatite (BHA). BHA is produced by means of deproteinizing by a high-temperature heating process so that inorganic material of bone is left where the bone architecture is preserved. This material has osteoconductive property because it induces osteoblast activity and new bone formation. DFDBX is a bone graft derived from bovine bone which has undergone the demineralization process and subsequently frozen. Then, it will be exposed to hydrochloric acid until the bone matrix component-related collagen fibril called BMPs. Runt-Related Transcription factor 2 (RUNX2) is a transcription factor which is needed for osteoblast differentiation and it is first detected at preosteoblast. Osteocalcin is exerted during the last stage of differentiation, started at the early stage of mineralization. Objectives to compare the expression of RUNX2 and Osteocalcin following the application of DFDBX and BHA to the bone defect. Methode30 male New Zealand White Rabbit, 6- months old, 3-3,5kg, divided into 3 groups comprising of 10 animals each, bone defect is created on each animal model. On group 1, DFDBX is applicated, BHA is on group 2, and control group with no graft application. After 2 weeks and 4 weeks following the animal model is terminated to retrieve a bone specimens for Immunohystochemistry examination. Result The expression of RUNX2 following the application of DFDBX and BHA showed a significant difference at week 2 but not showed at week 4. This research also found that the expression of osteocalcin did not show a significant difference at week 2 but showed a significant difference at week 4. ConclussionThis study demonstrate that bone healing process in DFDBX group is more effective than BHA.
Aliza Dewi Fortuna
Journal of Stem Cell Research and Tissue Engineering, Volume 5, pp 52-60; https://doi.org/10.20473/jscrte.v5i2.33144

Abstract:
The consumption rate of Monosodium Glutamate (MSG) in Indonesia has increased every year. Uncontrolled use of MSG in Indonesia for a long period of time can cause toxic effects on the body. The free glutamate content produced by MSG can affect the work of the immune system, especially in the innate immune system and cause oxidative stress. To determine the effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells. This study is a laboratory experimental in vitro with a post test only control group design. A total of 10cc of peripheral venous blood was isolated using the ficoll gradient centrifugation method. The results of monocyte cell isolates were exposed to Monosodium Glutamate (MSG) according to groups. Group I: negative control, group II: monocyte cells + MSG 3%, group III: monocyte cells + MSG 6%, group IV: monocyte cells + MSG 9%. Subsequently incubated for 24 hours at 37 °C in 5% CO2. Then the viability test was carried out using trypan blue staining. Monocyte cell viability calculations were carried out under an inverted microscope with a magnification of 400x per 100 cells. The data obtained were analyzed statistically using the one-way Anova test followed by the LSD test. The average viability in each group was obtained as follows, monocyte cell viability in the control group was 63%, group II was 47%, group III was 45% and group IV was 35%. There is an effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells with the most significant effect being the 9% MSG concentration with an average viability of 35%.The consumption rate of Monosodium Glutamate (MSG) in Indonesia has increased every year. Uncontrolled use of MSG in Indonesia for a long period of time can cause toxic effects on the body. The free glutamate content produced by MSG can affect the work of the immune system, especially in the innate immune system and cause oxidative stress. To determine the effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells. This study is a laboratory experimental in vitro with a post test only control group design. A total of 10cc of peripheral venous blood was isolated using the ficoll gradient centrifugation method. The results of monocyte cell isolates were exposed to Monosodium Glutamate (MSG) according to groups. Group I: negative control, group II: monocyte cells + MSG 3%, group III: monocyte cells + MSG 6%, group IV: monocyte cells + MSG 9%. Subsequently incubated for 24 hours at 37 °C in 5% CO2. Then the viability test was carried out using trypan blue staining. Monocyte cell viability calculations were carried out under an inverted microscope with a magnification of 400x per 100 cells. The data obtained were analyzed statistically using the one-way Anova test followed by the LSD test. The average viability in each group was obtained as follows, monocyte cell viability in the control group was 63%, group II was 47%, group III was 45% and group IV was 35%. There is an effect of exposure to Monosodium Glutamate (MSG) on the viability of monocyte cells with the most significant effect being the 9% MSG concentration with an average viability of 35%.
Zefry Zainal Abidin
Journal of Stem Cell Research and Tissue Engineering, Volume 5, pp 80-86; https://doi.org/10.20473/jscrte.v5i2.33149

Abstract:
Autogenous bone graft is the gold standard for bone defects treatment, however due to their limitation and the donor site morbidity may caused many surgeons use a xenograft type of bone grafting to cope the problem. Demineralized Freeze Dried Bone Xenograft (DFDBBX) which contains of growth factors, have a good biocompatibility. The aim of this study is observed the difference in bone healing processes between DFDBBX and Bovine Bone Hydroxyapatite Xenograft (BBHAX). Bicortical bone defects were created in the mandibular corpus of 30 New Zealand White Rabbits. The groups were divided into 3 groups which the first group were treated with DFDBBX into the hole and the negative control group was left perforated. The other group was treated with BBHAX. All group were evaluated after second and fourth weeks to count the ammount of osteoblast, osteoclast cells, Collagen-1 (Coll-1) and alkalin phosphatase (ALP). The second week of observation showed a significant difference of mean 12,45, SD 2,97 (p<0,05) in osteoblast cells. In Coll-1 showed with mean 13,2 SD 2,68 (p<0,05). The result of ALP showed with mean 14,6 SD 2,70 (p<0,05). In the the fourth week observation showed increased of osteoclast cells with mean 7,043, SD 2,77 (p< 0,05) and for Coll-1 with mean 17,6, SD 2,30 (p< 0,05). DFDBBX showed more effective in treating bone defects of mandible of new zealand white rabbits in second week of observation.
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