Journal of Stem Cell Research and Tissue Engineering

Journal Information
ISSN / EISSN : 2614-1264 / 2614-1256
Current Publisher: Universitas Airlangga (10.20473)
Total articles ≅ 35
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Gusti Revilla
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 70-80; doi:10.20473/jscrte.v4i2.22753

Abstract:
The therapy to heal the bum wound is still imperfect, therefore it is important to conduct specific research concerning this topic that benefits to society. Using both Bone Marrow-stem cell (BM-MSc) and peripheral blood mononuclear cells (PBMCs) from allogenic donors as part of the therapy to heal the bum wound seems to give positive prospect for the future treatment. In this experiment, PBMC and rat BM- derived from mesenchymal stem cell were used as the therapy model. lmmunocytochemistry was used as the method to characterize the phenotype of MSc, It was also used to express the collagen type I and the Indirect ELISA in analyzing the TGF·P 1 secretion. The rats with bum wound were divided into 2 kinds of group; the first group of rats was selected to control the use of PBS; while second group of rats was used as the treatment object that was medicated by the applying of the combination of both BM-MSC and PBMC. Stem-cells subcutaneously administered dose applied to each rat was around of 2 x 106 cells. The result showed that the levels of TGF-β1 secretion in day 3rd and day 7th on the rats which were treated by using the combination of BM-MSC and PBMC were higher compared to the rats from the control group. The experiment that concerns on the thickness of the collagen showed that combination between BM-MSC and PBMC stem cell make it possible in increasing the thickness of collagen 1, besides; it also showed significant differences (p=0.000). This research proved that the combination of BM-MSC and PBMC stem cell served can accelerate the healing process for the bum wound on rats through Increasing of TGF-P 1 secretion and collagen type 1 expression, It means that PBMCs can be applied as good as chemoattractant.
Deya Karsari
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 40-48; doi:10.20473/jscrte.v4i2.22748

Abstract:
This study aims to analyze genetic stability of the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.
Erma Safitri
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 64-69; doi:10.20473/jscrte.v4i2.22752

Abstract:
The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).
Hendi Hendarto
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 56-63; doi:10.20473/jscrte.v4i2.22751

Abstract:
Introduction: Chemotherapy has cytotoxic effect that induces follicular damage and abnormal folliculogenesis leads to ovarian failure. Two crucial growth factors in abnormal folliculogenesis, Growth Differentiation Factor-9 (GDF-9) and Kit-Ligand, will be disrupted and affect follicular development. In this study, we evaluate whether bone marrow transplantation (BMT) has a role on oocyte-granulosa cell interaction by analyzing GDF-9 and Kit-Ligand expressions and also follicular development by analyzing primordial, primary, secondary and graafian follicles of cisplatin-induced ovarian failure in rat. Material and method: Forty eight rats were divided into three groups: control, cisplatin and cisplatin + BMT. Ovarian failure was induced by administration of intraperitoneal cisplatin 5 mg/kg body weight for 1 week. BMT 2 x107 cells were injected through rat tail vein after cisplatin administration. Bone marrow was isolated from rat femur and characterized by CD44(+), CD45(-), CD105(+). Immunohistochemistry examinations for ovarian GDF-9, Kit-Ligand and follicle development evaluation were performed after 2 weeks of BMT injection. Results: The expressions of Kit-ligand among three groups by ANOVA were significant different (p=0.00), whereas by Post Hoc: cisplatin group lower than control group (p=0.00); cisplatin + BMT group higher than cisplatin group (p=0.00); and no significant different between control group and cisplatin + BMT group (p=0.955). The expressions of GDF-9 by Kruskal Wallis showed significant different (p=0.00) among three groups whereas cisplatin + BMT group higher than cisplatin group and control group. In cisplatin + BMT group the number of primordial, primary, secondary and graafian follicles were higher than those in cisplatin group; but were lower than those in control group (p=0.000). Positive Paul Kart Horan (PKH) labeling was seen in cisplatin + BMT group only. Conclusion: In cisplatin-induced ovarian failure in rat, bone marrow transplantation may improve oocytegranulosa cell interaction and follicular development.
Purwati Purwati
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 49-55; doi:10.20473/jscrte.v4i2.22749

Abstract:
Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HN). At the beginning of infection, gpl20 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gpI20 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell. Therefore, CCR5 arid CXCR4 also determine the fate of the target cell. It is the performance of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HN infection. Coding gene CCR5 may mutate to become protective or resistant against HTV infection. In homozygote individuals, it tends to be resistant against infection while in heterozygote individuals it tends to be susceptible to HN infection. Objective: To characterize TCD4 lymphocyte in the next that is resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HTV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HN and comparison to control. Result: This study was performed in several steps, such as mononucleated cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification. Conclusion: Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HN & AIDS treatment.
Fedik Rantam
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 1-9; doi:10.20473/jscrte.v4i1.21588

Abstract:
Infection with DENV causes a spectrum of clinical disease ranging. The aim of this study is to investigate the infectivity of DENV with degree of severity dengue infection in Surabaya. Dengue infection was established by IgM anti dengue, and two step multiplex RT PCR and Nucleotide sequence. Grading of degree severity infection follow the WHO criteria 2011. DSS cases found 3 from 36 patients caused by DENV 2. The most uninfective was DENV 1, and the most prevalence dengue infection caused by DENV 3. The infectivity of dengue infection shown 16 patients lead to severity with plasma leakage. All of sera patients detecting using multiplex RT-PCR were positive, but it were analyzed using Duo ELISA only 22 serum sera positive IgM and IgG from 36 sera. . The Phylogenetic analysis indicates that the isolates from 2011 to 2012 close related with dengue isolate from 1998 and belong to 2009 to 2020.In this study it indicates that DENV 2 predominantly is the cause of DSS.
Andika Djaja
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 10-16; doi:10.20473/jscrte.v4i1.21589

Abstract:
Fractures usually heal normally. In some conditions, the healing process do not occur normally, but become delayed union or non union as a complication. Successful healing of fractures is a complex interaction between the process of angiogenesis and osteogenesis (the interaction of osteoblasts and osteoclast). Physical stimuli such as exposure of EMF (electromagnetic fields) influences of the osteogenesis process both in the development stage of embryo reinforcement and the fracture healing stage. The aim of this study is to determine the healing of delayed union fractures in experimental animals due to the expossure of Extreme Low Frequency-Pulsed Electromagnetic Field (ELF-EMF) by comparing the RUST scores. The experimental study was conducted Department of Nutrition Animal Laboratory, Faculty of Medicine, Universitas Indonesia with 56 experimental rats during August-September 2018. There were no differences of animal characteristics in the study. It was found that there were significant differences in Rust Score in the treatment and control groups in each examination week. There were no differences in clinical improvement in the two groups. This study concluded that there was an improvement in delayed union fracture healing after the administration of ELF-EMF as seen from the difference in Rust score.
Deya Karsari
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 34-39; doi:10.20473/jscrte.v4i1.21597

Abstract:
The aim of this study was to isolate and identify Avian Influenza A non-H5 virus from muscovy duck at two live bird markets in Surabaya. Muscovy duck is the natural reservoir of Avian Influenza virus, in which all of the 16 HA subtypes and 9 NA maintained. The Avian Influenza virus replicates in intestinal tract of the reservoirs, causing the high amount of virus shed in the faeces. This study is an observational descriptive study, using non random sampling method of determined samples. The method used in this study were Hemagglutination Inhibition (HI) test and Enzyme Linked Immunosorbent Assay (ELISA) test. Avian Influenza A non-H5 virus was identified 19.23% (5 samples out of 26) in PS1 and 23.34% (7 samples out of 30) in PS2. This finding shows that Avian Influenza A non-H5 virus could be isolated and identified from muscovy duck at two live bird markets in Surabaya.
Racma Nisaa
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 26-33; doi:10.20473/jscrte.v4i1.21591

Abstract:
The quality of drinks is one of the problems that exist in society, especially because of bacterial contamination of drinks that often occurs in developing countries. One of them is soy milk drinks that are contaminated by bacteria including those sold by street vendors in the Pogot area of Surabaya. Based on the background description in this study can be formulated as follows: Is the number of Most Probable Number (MPN) Coliform in soy milk sold by street vendors in the Pogot area of Surabaya fulfill the Indonesian National Standard (SNI) 7388: 2009? The purpose of this study was to determine the amount of Coliform in soy milk sold by street vendors in the Pogot area of Surabaya compared to the Indonesian National Standard (SNI) 7388: 2009 regarding the maximum limit of microbial contamination in food. This study used a descriptive study design that soy milk sold by street vendors in the Pogot area of Surabaya was the population. Samples were used as many as 16 samples from 4 street vendors, each of which was taken 4 then laboratory-tested and analyzed using percentages. Based on the requirements of the Indonesian National Standard (SNI) 7388: 2009, the limit of contamination of the Most Probable Number (MPN) Coliform in soy milk is 20 / ml. Based on the results of the research of 4 soy milk traders there were 2 traders whose the results fulfilled the requirements of the Indonesian National Standard (SNI) and 2 traders whose the results didn't fulfill the requirements of the Indonesian National Standard (SNI). So 50% were eligible (MS) and 50% were not eligible (TMS).
David Kamadjaja
Journal of Stem Cell Research and Tissue Engineering, Volume 4, pp 17-25; doi:10.20473/jscrte.v4i1.21590

Abstract:
Amniotic membrane of human placenta is a source of abundant mesenchymal stem cell (hAMSC) which makes it a potential source of allogeneic multipotent cell for bone healing. However, much has to be explored about its isolation procedure and the osteogenic differentiation potential. The aims of this study are to establish the procurement procedure of human amniotic membrane, the isolation and culture of hAMSC, the MSC phenotypic characterization, and the in vitro osteogenic differentiation of hAMSC. Results of the study are as follows. The quality of human amniotic membrane would be best if procured from Caesarean operation under highly aseptic condition to avoid fungal and bacterial contamination on the culture. Isolation procedure using modified Soncini protocol yielded large amount of MSC with high proliferative capacity in culture medium. Characterization of hAMSC showed that the majority of the target cells exhibited specific MSC markers (CD10S and CD90) with a small number of these cells expressing CD45the marker of hematopoeitic cells. The in vitro osteogenic differentiation of hAMSC followed by Alizarin Red staining showed that osteoblastic differentiation was detected in a significantly high number of cells. This study concludes that hAMSCs isolated from human amniotic membrane have the capacity for in vitro osteogenesis which makes them be one of the potential allogeneic stem cells for application in maxillofacial bone reconstruction.
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