World Journal of Cancer Research

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ISSN / EISSN : 2164-9049 / 2164-9057
Published by: Hans Publishers (10.12677)
Total articles ≅ 126
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彤唐 瑀
World Journal of Cancer Research, Volume 12, pp 7-15; https://doi.org/10.12677/wjcr.2022.121002

Abstract:
# 白血病是一类造血干/祖细胞的恶性克隆性疾病,其治疗方法主要有化学治疗、造血干细胞移植、免疫治疗和分子靶向治疗等。近年来,白血病的免疫治疗取得了重大突破,成为研究热点,尤其是嵌合抗原受体修饰T细胞(chimeric antigen receptor T cells, CAR-T)作为新型治疗模式备受关注。CAR-T细胞的治疗基础为白血病细胞上的特异性抗原,即免疫治疗靶点。笔者对近年来白血病CAR-T细胞治疗靶点的相关研究进行综述。 Leukemia is defined as malignant neoplasm of blood-forming tissues, which characterized as abnormal proliferation of leukocytes. The main treatment includes chemotherapy, allogeneic hematopoietic stem cell transplantation, immunotherapy and cellular therapy. Recently, immunotherapy, is an emerging novel therapy and has achieved dramatic success in clinical practice, especially for chimeric antigen receptor (CAR) T-cell. The basic of CAR-T therapy is tumor specific antigen, this review summarizes related potential tumor antigens in leukemia for CAR-T therapy.
转达 德
World Journal of Cancer Research, Volume 12, pp 1-6; https://doi.org/10.12677/wjcr.2022.121001

Abstract:
# 我国肿瘤发病率与死亡率居高,以乳腺癌、肺癌为代表的恶性肿瘤的诊治面临巨大挑战。氧化应激是指由于活性物质的产生或氧化还原系统代偿能力有限使得细胞内氧化还原状态被破坏的一种病理过程。研究表明氧化应激与肿瘤中氧化应激水平多处于失衡状态并与恶性肿瘤发生发展密切相关。谷胱甘肽(glutathione, GSH)在肺癌、结直肠癌等多种肿瘤中异常表达且与氧化应激失衡相关。另外参与合成GSH的关键酶谷氨酸半胱氨酸连接酶(glutamate cysteine ligase, GCL)及其亚基也受到很大关注,他们可能是造成恶性肿瘤中GSH合成紊乱及氧化应激失衡的重要原因,并在不同肿瘤中发挥促癌或抑癌的作用。但是GCL及其亚基与肿瘤发生发展的关系、与患者临床病理特征的关系尚未系统阐述,该文就GCL及其亚基在肿瘤中的表达及其意义进行综述。 The incidence and mortality of cancer are high in China, but the diagnosis and treatment of malignant tumors represented by breast cancer and lung cancer are facing great challenges. Oxidative stress refers to a pathological process in which the redox state in cells is destroyed due to the production of active substances or the limited compensatory capacity of REDOX system. Studies have shown that oxidative stress is often unbalanced with the level of oxidative stress in tumors and is closely related to the occurrence and development of malignant tumors. Glutathione (GSH) is abnormally expressed in lung cancer, colorectal cancer and other tumors and is associated with oxidative stress imbalance. In addition, glutamate cysteine ligase (GCL) and its subunits, a key enzyme involved in the synthesis of GSH, have also attracted great attention, which may be an important cause of GSH synthesis disorder and oxidative stress imbalance in malignant tumors, and play a role in promoting or inhibiting cancer in different tumors. However, the relationship between GCL and its subunits and tumor development and clinicopathological features of patients has not been systematically described. Therefore, this paper reviews the expression and significance of GCL and its subunits in tumors.
婧周 文
World Journal of Cancer Research, Volume 12, pp 16-22; https://doi.org/10.12677/wjcr.2022.121003

Abstract:
# 目的:探讨子宫内膜癌患者淋巴结清扫术后并发盆腔淋巴囊肿的危险因素,以用于指导淋巴囊肿的防治。方法:选取于青岛大学附属医院行腹腔镜下淋巴结清扫术的168例子宫内膜癌患者作为研究对象,分析术后发生淋巴囊肿的危险因素。结果:淋巴囊肿发生率为26.8%;单因素分析结果显示,淋巴囊肿的发生与淋巴结切除数目、血清白蛋白水平、腹部手术史、大网膜切除、术中出血量具有相关性(P Objective: To investigate the risk factors of complicated pelvic lymph cyst after lymph node dissection in patients with endometrial cancer to guide the control of lymph cyst. Methods: The 168 cases of endometrial cancer patients undergoing laparoscopic lymph node dissection of Qingdao were selected to analyze the postoperative risk factors of lymphocyst. Results: The incidence of lymphocysts was 26.8%; univariate analysis showed that the number of lymphadenectomies, serum albumin level, abdominal surgery, omentectomy, intraoperative amount of bleeding (P < 0.05); multiple logistic regression analysis showed that the number of lymphadenectomy and serum albumin levels were independent risk factors for lymphadenectomy in patients with endometrial cancer. Conclusion: The incidence of pelvic lymph cyst is high after lymph node dissection, and the number and range of lymphadenectomy, serum albumin levels are their independent risk factors, which should be paid attention to and prevented.
星金 博
World Journal of Cancer Research, Volume 11, pp 97-105; https://doi.org/10.12677/wjcr.2021.113013

Abstract:
目的:借助科学文献可视化软件Citespace对我国人前列腺癌的研究现状、研究热点及发展趋势等进行综合全面的汇总与分析,旨在为人体前列腺癌的研究提供指导与参考。方法:以CNKI数据库为检索对象,在文献分类目录中检索“医药卫生科技”分类目录下的“基础医学”、“临床医学”、“肿瘤学”和“生物医学工程”研究方向中1999~2018年所收录的期刊论文,应用Citespace可视化软件对作者、机构、关键词等进行分析。结果:通过对21,262篇前列腺癌期刊文献进行分析,我们发现前列腺癌研究领域的发文量逐年增长,获得了较多的研究关注。我国对前列腺癌的研究模式主要是以高校以及附属医院为主,研究的内容与社会的需求很好地契合。研究热点逐渐由早期的化疗以及抗雄激素治疗转向近年来的前列腺癌根治术和早期诊断,使得将来对前列腺癌的预防和根除成为可能。 Objective: To comprehensively summarize and analyze the progress, hotspots and trends of prostate cancer research in China, we use the scientific literature visualization software Citespace to provide guidance and reference for the research of human prostate cancer. Methods: The CNKI database was used as the search object, and the journal papers collected from 1999 to 2018 in “basic medicine”, “clinical medicine”, “oncology” and “biomedical engineering” were searched in the literature classification catalogue, and the authors, institutions and keywords were analyzed by Citespace visualization software. Results: Through analysis of the retrieved 21,262 journal literatures, we found an increasing annual amount of articles published in the research field of prostate cancer, indicating that intensive attention has been attracted to prostate cancer research. Majority of research teams are from affiliated hospitals of university. Research interests are in good agreement with the needs of society. Hotspots have been switched from previous chemotherapy and anti-androgen therapy to current radical prostatectomy and early diagnosis, which makes it possible to prevent and eradicate prostate cancer in the future.
姜 梅
World Journal of Cancer Research, Volume 11, pp 112-119; https://doi.org/10.12677/wjcr.2021.113015

Abstract:
目的:探讨益肺解毒方(YFJDF)含药血清逆转人肺腺癌A549/DDP细胞耐药性及诱导其凋亡的机制。方法:制备YFJDF含药血清,CCK8法分别检测不同浓度的顺铂对A549、A549/DDP细胞的增殖抑制率,不同浓度YFJDF对A549、A549/DDP细胞的增殖抑制率,YFJDF联合各浓度顺铂对A549/DDP细胞的增殖抑制率,并根据结果计算IC50值、A549/DDP细胞对顺铂的耐药指数及YFJDF逆转A549/DDP细胞的逆转耐药倍数。流式细胞术检测各组细胞凋亡率、West-blot检测各组细胞Bax、Bcl-2蛋白的表达水平。结果:不同浓度的DDP作用A549、A549/DDP细胞48 h后,其IC50值分别为4.49 μg/mL和45.09 μg/mL,计算出的耐药指数为10.05倍。不同浓度YFJDF对A549/DDP细胞的增殖有抑制作用,并且随着YFJDF浓度的升高和药物作用时间的延长对A549/DDP细胞的抑制作用也逐渐增加,选择中剂量的YFJDF、并作用48 h用于后续逆转实验。YFJDF联合各浓度顺铂对A549/DDP细胞的抑制作用更为明显,其48 h的IC50值为29.37 μg/mL,逆转倍数为1.53倍。流式细胞仪检测结果显示:YFJDF + DDP组细胞凋亡率显著增加,可达到69.3%;Western blot结果显示:YFJDF + DDP组Bax的表达量显著增加、Bcl-2的表达量明显减少。结论:益肺解毒方能逆转A549/DDP细胞对顺铂的耐药,其机制可能是促进顺铂诱导A549/DDP凋亡。 Objective: To study the reversal effect of Yifei Jiedu Decoction on cisplatin resistant human lung adenocarcinoma cell line A549/DDP and to explore the mechanism of induce cell apoptosis. Methods: Prepare YFJDF medicated serum. The method of CCK8 was used to detect the proliferation inhibition rate of different concentrations of DDP on A549 and A549/DDP cells, the proliferation inhibition rate of different concentrations of YFJDF on A549 and A549/DDP cells and YFJDF combined with different concentrations of DDP on A549/DDP cells. IC50, the drug resistance index of A549/DDP cells to DDP and the reversal drug resistance fold of YFJDF were calculated according to the results. Apoptosis rate in each group detected by flow cytometry. The expression level of apoptosis-related protein Bax and Bcl-2 was detected by western blot in each group. Results: After different concentrations of DDP acted on A549 and A549/DDP cells for 48 hours, the IC50 was 4.49 μg/mL and 45.09 μg/mL, drug resistance index was 10.05 times. Different concentrations of YFJDF have an inhibitory effect on the proliferation of A549/DDP cells, and with the increase of YFJDF concentration and the prolongation of drug action time, the inhibitory effect on A549/DDP cells will gradually increase. A medium dose of YFJDF was selected and the action time of 48 hours was selected for the subsequent reversal experiment. The inhibitory effect of YFJDF combined with DDP at various concentrations on A549/DDP cells was more obvious, and its 48 h IC50 was 29.37 μg/mL, and the reversal drug resistance fold was 1.53 times. The results of flow cytometry showed that the apoptosis rate of the YFJDF + DDP group increased significantly, reaching 69.3%; Western blot results showed that the expression of Bax in the YFJDF + DDP group increased significantly, and the expression of Bcl-2 decreased significantly. Conclusion: Yifei Jiedu Decoction can reverse the resistance of A549/DDP cells to DDP, and its mechanism may be to promote the apoptosis of A549/DDP induced by DDP.
梅魏 冬
World Journal of Cancer Research, Volume 11, pp 1-7; https://doi.org/10.12677/wjcr.2021.111001

Abstract:
目的:探讨养阴扶正方对肺癌小鼠体内HIF-1α、Arg1表达的影响。方法:建立Lewis肺癌C57BL/6鼠移植瘤模型,建模成功后,分别予以养阴扶正方低、中、高剂量组进行干预;绘制肿瘤生长曲线,计算抑瘤率,Elisa方法检测瘤组织中乳酸的含量,West-blot、Real-time分别检测Lewis肺癌HIF-1α、Arg1的蛋白及mRNA表达。结果:Lewis肺癌移植瘤模型建立;养阴扶正方可抑制Lewis肺癌生长,降低Lewis肺癌乳酸,减瘤组织HIF-1α、Arg1的mRNA的表达。结论:养阴扶正方改善了肺癌的酸性微环境,通过对HIF-1α、Arg1的调控,减少M2型TAMs的数量,降低免疫抑制蛋白因子的分泌,逆转了免疫抑制,抑制了肺癌的生长,是养阴扶正方抗NSCLC的新机制。 Objective: Discuss the effects of Yangyin Fuzheng Decoction on HIF-1α and Arg1 expression in lung cancer mice. Method: Establish the model of Lewis lung cancer C57BL/6 mouse transplantation tumor; the low, medium and high dose of Yangyin Fuzheng Decoction were given for intervention after modeling successfully. Draw the tumor growth curve, calculate tumor-inhibition rate, detect the lactic acid content of with Elisa, test HIF-1α, Arg1 protein and mRNA expression of Lewis lung cancer with West-blot and Real-time respectively. Result: The establishment of Lewis lung cancer transplantation tumor model establishment; Yangyin Fuzheng Decoction can inhibit the growth of Lewis lung cancer, decrease Lewis lung cancer lactic acid and reduce mRNA expression of tumor tissue HIF-1αand Arg1. Conclusion: Yangyin Fuzheng Decoction improves the acidic microenvironment of lung cancer by regulating HIF-1αand Arg1, reduces the amount of M2 style TAMs, decreases the secretion of immunosuppressive protein factor, reverses the immunosuppression and inhibits the growth of lung cancer, which is an Anti-NSCLC new mechanism of Yangyin Fuzheng Decoction.
桐刘 心
World Journal of Cancer Research, Volume 11, pp 67-75; https://doi.org/10.12677/wjcr.2021.113009

Abstract:
目的:检测NEK6在HepG2细胞中的定位,肝细胞肝癌组织与正常肝脏组织中的表达水平,分析其与临床病理学资料间的关系。方法:在HepG2细胞中转染pMCV-Nek6-Myc和pMCV-Nek6-Flag,应用间接免疫荧光法测定NEK6在HepG2细胞中的定位;应用RT-qPCR法检测47例肝细胞肝癌(HCC)组织与相应远癌旁组织(>1 cm)中NEK6 mRNA的表达水平;应用Western blot法检测该47例肝细胞肝癌组织与相应正常肝组织中NEK6蛋白的表达水平。收集并统计该组患者的临床病理学资料,包括性别、年龄、肿瘤大小、肿瘤数量(单个/多个)、血清AFP水平(≥400 ng/ml或 Objective: To detect the localization of NEK6 in HepG2 cells, the expression level of hepatocellular carcinoma tissues and normal liver tissues, and to analyze the relationship between NEK6 and clinicopathological data. Methods: HepG2 cells were transfected with pMCV-Nek6-Myc and pMCV-Nek6-Flag, and the localization of NEK6 in HepG2 cells was determined by indirect immunofluorescence method. RT-qPCR was used to detect the expression level of NEK6 mRNA in 47 cases of hepatocellular carcinoma (HCC) tissues and corresponding distant adjacent tissues (>1 cm). Western blot was used to detect the NEK6 protein expression levels in the 47 cases of hepatocellular carcinoma tissues and corresponding normal liver tissues. The clinical pathological data of this group of patients were collected and counted, including gender, age, tumor size, tumor number (single/multiple), serum AFP level (≥400 ng/ml or <400 ng/ml), Edmondson grade, fiber envelope , Microvascular invasion (microvascular invasion MVI), etc., to analyze the relationship between NEK6 mRNA expression level and various clinicopathological characteristics. Paired samples were tested by paired t test, chi-square test was used for univariate analysis, and binary logistic regression analysis was used for multivariate analysis. The results were considered statistically significant at P < 0.05. Results: Immunofluorescence experiments showed that NEK6 was mainly located in the nucleus of HepG2 cells, and a small amount in the cytoplasm; RT-qPCR results showed that the expression of NEK6 mRNA in HCC was (1.405 ± 0.472), which was significantly higher than that of normal liver tissue: the expression level in HCC (1.133 ± 0.474); the difference was statistically significant (t = 3.143, P = 0.0029); Western blot results showed that the expression level of NEK6 protein in HCC was (1.031 ± 0.193), which was significantly higher than that of normal liver. The expression in tissues (0.906 ± 0.182) has a statistically significant difference (t = 3.464, P = 0.0012). Conclusion: NEK6 mainly exists in the nucleus of HepG2 cells, and its expression is up-regulated in hepatocellular carcinoma. Microvascular invasion is an independent factor related to the expression of Nek6 and may be used as one of the indicators of poor prognosis of liver cancer.
丽严 双
World Journal of Cancer Research, Volume 11, pp 24-31; https://doi.org/10.12677/wjcr.2021.111004

Abstract:
在我国,结直肠癌发病率呈逐年增加趋势,靶向药物联合化疗已成为晚期结直肠癌治疗的趋势所在,近几年随着靶向药物治疗的应用,很大程度上延长了晚期结直肠癌患者的生存期。同时,由于对结直肠癌病因学、分子生物学、细胞学的不断升入研究及相关试验开展,新的靶向药物不断涌现。本文综述了结直肠癌靶向治疗的临床应用与研究进展。 In China, the incidence rate of colorectal cancer is increasing year by year. Targeted drug combination chemotherapy has become the trend of treatment for advanced colorectal cancer. In recent years, with the application of targeted drug therapy, the survival time of patients with advanced colorectal cancer has been greatly extended. At the same time, with the development of the research on the etiology, molecular biology and cytology of colorectal cancer, new targeted drugs are emerging. This paper reviews the clinical application and research progress of targeted therapy for colorectal cancer.
莹王 梦
World Journal of Cancer Research, Volume 11, pp 121-125; https://doi.org/10.12677/wjcr.2021.114016

Abstract:
费城染色体阳性急性髓系白血病(Ph+AML)是一种罕见的AML亚型,2016年修订的世界卫生组织(WTO)髓系恶性肿瘤将其归类为预后不良伴有遗传学异常的AML暂定型。Ph+AML是有别于慢性髓系白血病的一组高度复杂的异质性疾病,对化疗反应差,易复发,预后不佳,具有独特的临床和血液学特点。 Philadelphia chromosome-positive acute myeloid leukemia (Ph+AML), as a rare subtype of AML, is classified as provisional AML of poor prognosis accompanied with genetic abnormalities according to the 2016 revision of World Health Organization (WTO)-defined myeloid malignancy. Ph+AML is a group of highly complex heterogeneous diseases different from chronic myeloid leukemia (CML) with poor response to chemotherapy. It is characterized by easy recurrence, poor prognosis, and unique clinical and hematological features.
江 杨
World Journal of Cancer Research, Volume 11, pp 48-58; https://doi.org/10.12677/wjcr.2021.112007

Abstract:
目的:评价EB病毒、幽门螺杆菌感染与胃癌组织中表皮生长HER2、P53、Ki-67表达的相关性,初步探讨两者感染在胃癌发生发展的相关作用。方法:收集我院行胃癌根治手术病例的组织蜡块共78例,采用原位杂交方法检测胃癌组织中EBV感染情况,利用免疫组织化学方法检测胃癌组织中Hp感染状态以及HER2、P53、Ki-67等蛋白表达情况。结果:EBV感染阳性率为12.8% (10/78),Hp阳性率为57.7% (45/78),EBV、Hp两者感染不存在一致性;胃癌组织中EBV、Hp感染分别与性别、年龄、淋巴结有无转移、胃腺癌分化程度、浸润深度、临床分期无显著差异;HER2、P53、Ki-67等蛋白表达在胃癌组织中与性别、年龄、淋巴结有无转移、腺癌分化程度、浸润深度、临床分期无显著差异;P53、Ki-67蛋白表达与Hp感染有显著性差异,P53、Ki-67蛋白表达与Hp感染存在正相关关系,Hp阳性组与Hp阴性组HER2蛋白表达无显著性差异;EBV阳性或Hp阳性组与两者联合感染组中P53、Ki-67蛋白表达差异无统计学意义。结论:Hp感染组与非感染组胃癌组织中P53、Ki-67表达存在显著差异,且Hp感染与P53、Ki-67表达呈正相关,胃癌组织中Hp感染可能促进上述蛋白表达;胃癌组织中EBV或Hp感染和EBV、Hp联合感染分别对HER2、P53、Ki-67蛋白表达无增强作用,两者之间无相关性,EBV、Hp可能是胃癌发生发展中的两个独立因素。 Objective: This study is aimed to evaluate the correlation between Epstein-Barr virus, Helicobacter pylori infection and the expression of human epidermal growth factor receptor-2, P53 and Ki-67 in Gastric Cancer tissues, which is helpful to determine the correlation between those two pathogen and gastric cancer. Methods: A total of 78 cases of tissue wax blocks for radical gastric cancer surgery in our Hospital were collected. In situ hybridization was used to detect EBV infection in gastric cancer tissues, and immunohistochemical methods were used to detect the status of HP infection and the expression of HER2, P53, Ki-67 and other proteins in gastric cancer tissues. Results: The positive rate of EBV infection was 12.8% (10/78) and the positive rate of HP was 57.7% (45/78). There is no consistency between the two infections. There were no significant differences between EBV and HP infections in gastric cancer tissues, including gender, age, lymph node metastasis, gastric adenocarcinoma differentiation, depth of invasion, and clinical stage. The expression of HER2, P53, Ki-67 and other proteins in gastric cancer tissues were not significantly different from sex, age, lymph node metastasis, adenocarcinoma differentiation, invasion depth, and clinical stage. The expressions of P53 and Ki-67 proteins were significantly different from Hp infection. There was a positive correlation between the expression of P53 and Ki-67 and Hp infection. There was no significant difference in HER2 protein expression between Hp-positive group and Hp-negative group. There was no significant difference in the expression of P53 and Ki-67 protein between EBV positive or HP positive group and EBV combined HP infection group. Conclusion: There were significant differences in the expression of P53 and Ki-67 in gastric cancer tissues between the HP infection group and the non-infection group, and Hp infection was positively correlated with the expression of P53 and Ki-67. Hp infection in gastric cancer tissues may promote the above protein expression. EBV or HP infection and EBV and Hp infection in gastric cancer tissues have no effect on the enhancement of P53 and Ki-67 protein expression, respectively, and there is no correlation between them. EBV and Hp may be two independent factors in the development of gastric cancer.
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