Hans Journal of Biomedicine
ISSN / EISSN : 2161-8976 / 2161-8984
Published by: Hans Publishers (10.12677)
Total articles ≅ 134
Latest articles in this journal
Hans Journal of Biomedicine, Volume 11, pp 129-133; https://doi.org/10.12677/hjbm.2021.113017
目的：测定本校生命学院大三本科生苯硫脲(PTC)味盲基因频率。方法：采用阈值法测定250名本科生对PTC的尝味敏感性。结果 味盲率为10.4%，味盲基因频率为0.356，尝味基因频率为0.644。结论：尝味能力与民族、地理位置以及某些疾病相关。 Objective: To determine the taste-blindness gene frequency of PTC among undergraduate students in our college of life science. Methods: To adopt the way of deciding taste-blindness with threshold to determine the sensitivity to the taste on PTC of 250 juveniles. Result: The taste-blindness rate was 10.4%, the taste-blindness gene frequency was 0.356 and the taste frequency was 0.644. Con-clusion: The PTC tasting ability is relevant to nationality, geographic position and some diseases.
Hans Journal of Biomedicine, Volume 11, pp 134-141; https://doi.org/10.12677/hjbm.2021.113018
由病原微生物引起的疾病以及使用常规抗菌剂治疗对社会经济、环境和健康的影响是日益增加的。对抗生素的抗药性越来越强，许多普通抗生素对人类健康和免疫系统产生了有害的生物副作用，这促使人们寻找新的成本效益高、效率高的消毒处理方法和对免疫力有改善的方法。低频活化水(等离子体活化水)是冷大气等离子体与水反应的产物，是一种很有科研发展前景的广谱杀菌剂，低频共振装置发射的舒曼低频的7.83赫兹复制大地磁场的频率，舒曼共振与身体健康息息相关，7.83赫兹的舒曼波与人体脑电波及α波的频率基本接近,它对提高人体免疫力，辅助改善亚健康，激活细胞等方面有一定作用。低频活化水的瞬时活性，即低频活化水在储存和应用后几天内就会恢复到水中，用低频活化水处理大肠杆菌导致超过4-log的减少，该研究成功地证明了低频活化水作为一种有效的环境良性消毒剂的可能性，为优化低频活化水的生化活性和将这种灭毒机制转化为生活中的应用提供了极大的依据。 Diseases caused by pathogenic microorganisms and the use of conventional antimicrobial treatments have an increasing impact on social economy, environment and health. The resistance to antibiotics is getting stronger and stronger. Many common antibiotics have harmful biological side effects on human health and the immune system. This has prompted people to find new cost-effective and efficient disinfection methods and methods to improve immunity. Low-frequency activated water (plasma activated water) is the product of the reaction between cold atmospheric plasma and water. It is a broad-spectrum fungicide with promising scientific research and development. The Schumann low-frequency 7.83 Hz emitted by the low-frequency resonance device replicates the frequency of the geomagnetic field. Schumann resonance is closely related to physical health. The Schumann wave of 7.83 Hz is basically close to the frequency of human brain waves and alpha waves. It has a certain effect on improving human immunity, assisting in improving sub-health, and activating cells. The instantaneous activity of low-frequency activated water, that is, the low-frequency activated water will be restored to the water within a few days after storage and application. Treatment of E. coli with low-frequency activated water resulted in a reduction of more than 4-log. This study successfully proved that low-frequency activated water can be used as The possibility of an effective environmental benign disinfectant provides a great basis for optimizing the biochemical activity of low-frequency activated water and transforming this detoxification mechanism into daily applications.
Hans Journal of Biomedicine, Volume 11, pp 40-48; https://doi.org/10.12677/hjbm.2021.112006
在多种逆境条件下，真核生物通常会在细胞质中形成呈聚集状颗粒的mRNA-蛋白质复合体，即应激颗粒(stress granules, SGs)。SGs的形成具有重要生理意义，包括通过调控mRNA翻译以及抑制细胞凋亡相关信号通路等机制，将逆境压力对细胞造成的损伤最小化，并促进细胞对逆境条件的适应以及在逆境条件下的存活。研究表明，SGs与多种疾病的发生关联紧密，包括多种类型的肿瘤、神经退行性疾病以及病毒感染相关疾病等。肿瘤细胞利用SGs促进了逆境条件下的存活，而一些神经退行性疾病中蛋白聚集体的形成也与异常SGs密切相关。因此，SGs逐渐成为以上疾病的新型药物靶点并受到越来越多研究的关注，SGs抑制剂也就应运而生。本文主要就靶向SGs的化合物及其在相关疾病中的应用进行了总结和探讨，以期为SGs抑制剂的研究及其临床应用提供参考。 Upon a variety of adverse conditions, eukaryotic cells usually form aggregating droplet-like mRNA-protein complexes namely stress granules (SGs) in the cytoplasm. The physiological signifi-cance of SGs formation is to minimize stress-related damage, promote stress adaptation and cell survival via regulation of mRNA translation and inhibition of apoptosis-related signaling pathways. Accordingly, dysregulation of SGs are closely associated with many diseases, including many types of cancer, neurodegenerative diseases and viral infectious diseases SGs hijacked by cancer cells promote the survival of cancer cells under adverse conditions, while protein aggregates found in some neurodegenerative diseases are also related to abnormal SGs. Therefore, SGs attract the at-tention of growing studies as a new therapeutic target, concomitantly with the emergence of SGs in-hibitors. This review will summarize and discuss recent progress on compounds targeting SGs and their application in SGs-related diseases, which may be helpful for research and therapeutic appli-cation of SGs inhibitors.
Hans Journal of Biomedicine, Volume 11, pp 106-112; https://doi.org/10.12677/hjbm.2021.112014
蛋氨酸是人体必需氨基酸，参与蛋白质和体内多种具有重要功能的生物分子的合成。近年来在多种模式生物中发现限制膳食中蛋氨酸的摄入可延长寿命，促进代谢健康，延缓或改善多种慢性衰老相关疾病的发生发展。本文简要概括了近年来膳食蛋氨酸限制健康促进作用和相关机制的进展，以其对进一步研究和应用提供有益线索。 Methionine is an essential amino acid for human that plays critical roles in the syntheses of many important biological molecules including protein. Recent studies have demonstrated that dietary methionine restriction can prolong lifespan, improve metabolic heath, prevent and postpone the development of some age-related chronic diseases in various model organisms. In this review, we summarized the recent progression of researches regarding the health promotion effects of dietary methionine restriction and the underlying mechanism, and expected to provide clues for further research and application.
Hans Journal of Biomedicine, Volume 11, pp 63-68; https://doi.org/10.12677/hjbm.2021.112009
异位辅助性肝移植比起原位肝移植有着许多独有的优势，其在急性肝衰竭、遗传代谢性肝病、终末期肝病等治疗上进步显著。但是腹腔空间的缺乏、门静脉血流竞争导致移植肝衰竭、移植早期的急性排斥反应等难题，阻碍了该术式更好的发展。本文就异位辅助性肝移植的目前现状及难点问题作一综述，以期进一步增加对异位辅助性肝移植的认识，为肝移植开拓新的思路。 Heterotopic auxiliary liver transplantation has many unique advantages over orthotopic liver transplantation in the treatment of acute liver failure, inherited metabolic liver disease, end stage liver disease and so on. However, the lack of abdominal space, portal vein blood flow competition lead to graft failure, early acute rejection and other problems, which hinder the better development of this technique. This article reviews the current status and difficulties of heterotopic auxiliary liv-er transplantation, in order to improve the understanding of heterotopic auxiliary liver transplan-tation and open up a new way for liver transplantation.
Hans Journal of Biomedicine, Volume 11, pp 55-62; https://doi.org/10.12677/hjbm.2021.112008
目的：观察盐霉素对人胶质母细胞瘤(U87)细胞增殖、凋亡的影响，检测盐霉素对凋亡相关基因表达影响以探究对U87细胞抑制机制。方法；分别用高浓度(SH, 4 μmol/L)、低浓度盐霉素(SL, 2 μmol/L)处理U87细胞，分为空白对照组(NC组)、SH组、SL组，48小时后，通过集落形成实验观察盐霉素对U87细胞的集落形成能力的影响。通过RT-PCR检测SENP2等33个凋亡相关下调基因RNA的表达水平。结果：集落形成实验结果显示，SH和SL组均能抑制U87的增殖，且成浓度依赖性(P Objective: To observe the effect of salinomycin on the proliferation and apoptosis of human glio-blastoma U87 cells, and to detect the effect of salinomycin on the expression of apoptosis-related genes to explore the mechanism of inhibition on U87 cells. Methods: U87 cells were treated with high concentration (SH, 4 μmol/L) and low concentration salinomycin (SL, 2 μmol/L), and divided into NC group, SH group, and SL group. After 48 hours, observe the effect of salinomycin on the col-ony forming ability of U87 cells through the colony formation experiment. RT-PCR was used to de-tect the RNA expression level of 33 apoptosis-related down-regulated genes such as SENP2. Results: The results of MTT and colony formation experiments showed that both SH and SL groups could in-hibit the proliferation of U87 in a concentration-dependent manner (P < 0.05). Flow cytometry re-sults showed that the apoptosis rate in SH group increased. PCR results showed that the expression of 33 apoptosis-related genes such as SENP2 was down-regulated. Conclusion: Salinomycin can sig-nificantly inhibit the proliferation of U87 cells and promote cell apoptosis.
Hans Journal of Biomedicine, Volume 11, pp 1-7; https://doi.org/10.12677/hjbm.2021.111001
长期暴露于高压环境下将影响大脑中具有调控情绪、行为表现的单胺类神经传导物质如多巴胺和血清素的浓度。精神益生菌被认为可在肠道中刺激神经传导物质的产生，再经由脑肠轴线的途径而对心理或行为方面产生正面有益的帮助，因此可利用身心压力的方式诱导类忧郁发生的模式来进行精神益生菌的筛选作业。在实验中，选用了ICR小鼠进行益生菌喂食两周后执行两周禁锢之测试，区分为禁锢组(RS)与乳酸菌补充之组别。比较每日管喂1.0 × 109 CFU乳酸菌株对禁锢鼠产生的影响。实验结束后牺牲小鼠，检测脑部中多巴胺与血清素的浓度，发现摄取GKF3四周后的小鼠脑中多巴胺与血清素显着的增加(p Long-term exposure to stress can affect the concentration of monoamine neurotransmitters such as dopamine (DA) and serotonin (5-HT) in the brain which is linked to certain symptoms of depression. Psychobiotics, the probiotics which stimulate the neurotransmitters via gut-brain axial and benefit the mental health, were screened by the stress-induced depressive-like behavior in mice. Twen-ty-five ICR mice were divided into five groups as follows: restraint-stressed group (RS), RS+ probi-otics Lactobacillus plantarum GKM3 group, RS+ probiotics L. reuteri GKR1 group, RS+ probiotics L. fermentum GKF3 group, and RS+ probiotics L. plantarum group (isolated from a commercial prod-uct). After two weeks of probiotics consumption at dosage 1.0 × 109 CFU/mice/day, mice were im-mobilized in restrainers for two hours once a day for two weeks. The probiotics strain GKF3 was se-lected as shown significant increases in levels of DA and 5-HT in the brain after four weeks of the experiment (p < 0.05). These results suggested the potential applications of psychobiotics, L. fer-mentum GKF3, in the prevention of depression-related brain diseases.
Hans Journal of Biomedicine, Volume 11, pp 75-86; https://doi.org/10.12677/hjbm.2021.112011
情绪是人对外界或自我刺激产生的心理和生理反应。如果人机交互(Human Computer Interaction, HCI)系统可以识别情绪，那么心理疾病的诊断和心理学的研究将会更加客观和有效。本文提出了一种仅基于光电容积脉搏波(photoplethysmography, PPG)的情绪识别方法对情感进行分类。由多波长近红外透射光谱法测量获得的脉搏波，经过特征点检测，获得其信号特征，使用不同的机器学习算法验证由PPG信号识别情绪的性能。结果表明，使用脉搏波进行情绪四分类，其识别正确率为96.2%，且单个样本的测试时间短。这意味着基于PPG信号可以实现快速的多类情绪识别，对无创检测、可穿戴设备和临床实践具有潜在的价值。 Emotion is person’s psychological reaction to external or self-stimulation with the physiological re-action. If Human Computer Interaction (HCI) system can be used to recognize emotion, the diagnosis of mental illness and psychological research will be more objective and effective. In this paper, an emotion recognition method based on photoplethysmography (PPG) is proposed to classify emo-tions. The signals measured by multi-wavelength near-infrared transmission spectroscopy are de-tected by feature points, and the signal characteristics are obtained. Different machine learning al-gorithms are used to verify the performance of emotion recognition by PPG signals. The results show that when PPG is used for emotion classification, the accuracy of recognition is 96.2%, and the testing time of a single sample is short. This means that fast multi-class emotion recognition can be achieved based on PPG signals, which has potential value for non-invasive detection, wearable de-vices and clinical practice.
Hans Journal of Biomedicine, Volume 11, pp 168-175; https://doi.org/10.12677/hjbm.2021.113022
CXXC锌指蛋白5 (CXXC finger protein 5, CXXC5)属于CXXC锌指蛋白家族。CXXC5基因是视色素反应基因，编码视色素诱导的核因子，又可称为RINF。CXXC5除了作为表观遗传调控因子外，还可以通过调控Wnt/β-catenin通路参与细胞的生长和分化过程；CXXC5多种恶性肿瘤的发生发展相关，可以作为TGF-β的靶基因，可活化激活TNF-α，参与调控肿瘤细胞的生长代谢过程，CXXC5表达情况对肿瘤治疗的预后也有参考作用；CXXC5对氧反应元件起抑制作用，参与细胞能量代谢等生物学过程。本文对近年来CXXC5的研究进展作一综述。 CXXC finger protein 5 (CXXC5) belongs to the CXXC zinc finger protein family.CXXC5 gene, also known as RINF, is a visual pigment response gene that encodes the nuclear factor of visual pigment induc-tion. In addition to acting as an epigenetic regulator, CXXC5 also participates in the process of cell growth and differentiation by regulating the Wnt/β-catenin pathway. CXXC5 is related to the occur-rence and development of a variety of malignant tumors. It can be used as a target gene of TGF-β, activate TNF-α, and participate in the regulation of the growth and metabolism of tumor cells. The expression of CXXC5 also plays a reference role in the prognosis of tumor therapy. CXXC5 inhibits oxygen response elements and participates in biological processes such as cell energy metabolism. In this paper, the research progress of CXXC5 in recent years is reviewed.
Hans Journal of Biomedicine, Volume 11, pp 187-194; https://doi.org/10.12677/hjbm.2021.114024
为实现精神疲劳状态的客观量化连续监测，本研究基于心率变异性的特征信息，研究了不同疲劳状态的量化识别方法。设计实验采集了不同疲劳状态下的心电信号，采用小波变换去除噪声干扰，提取了心率变异性的时域、频域等特征参数，并对所提取的特征参数对比分析以构建支持向量机等多种分类模型，最终利用支持向量机算法实现了正常、疲劳、嗜睡三种不同程度的精神状态分类。结果表明，本方法对于疲劳状态识别具有较高准确性，通过优化支持向量机的参数，对于不同疲劳状态的识别精度可超过80%。将此技术运用到日常生活中，可对人的主观疲劳状态进行客观化评价，在交通、教育、医学监护等方面具有重要的研究意义和应用前景。 In order to achieve the objective quantitative continuous monitoring of mental fatigue state, the quantitative identification method of different fatigue states is studied based on the characteristic information of heart rate variability. The ECG signals under different learning duration were col-lected in the design experiment. Wavelet transform was used to reduce the noises or interference, and characteristic parameters of heart rate variability (HRV) were extracted in the time domain and frequency domain. The extracted characteristic parameters were compared and analyzed to construct various classification models such as support vector machine. Finally, three mental states of normal, fatigue and drowsiness were classified by support vector machine algorithm. The results show that this method has a high accuracy for fatigue state identification, and the identification ac-curacy of different fatigue state can be more than 80% by optimizing the parameters of support vector machine. The application of this technology in daily life can objectively evaluate people’s subjective fatigue state, which has important research significance and application prospect in transportation, education, medical monitoring and so on.