Archives of Microbiology & Immunology
Latest articles in this journal
Archives of Microbiology & Immunology, Volume 5, pp 151-153; doi:10.26502/ami.93650054
We report therein the onset of three chronic diseases: Behçet's disease, fibromyalgia and rheumatoid arthritis in three sisters after tick bites. Only Behcet's disease was treated in our department. After failure of the initial treatment including colchicine and corticoids, the patient recovered within a prolonged antibiotic therapy time period of 18 months. A 23-year-old female patient was admitted to hospital in the Infectious Diseases Department as she presented with headache, diffuse inflammatory arthromyalgia, abdominal pain and cutaneous folliculitis, and for the umpteenth time for several past years, meningeal syndrome recurrence. The physical examination was unremarkable. The biological test assessment was normal, as were the ophthalmological and cerebral MRI examinations. The patient’s HLA B5 test was negative. The upper digestive fibroscopy shown digestive ulcerations. The diagnosis of Behçet's disease was made on the following: inflammatory arthralgias, digestive ulcerations, folliculitis lesions; recurrent bipolar aphthosis was found at patient’s interview. The patient was given prednisone 0.5 mg/kg/day and colchicine 1 mg/day. The evolution was characterized by an initial improvement of clinical symptoms followed by further iterative recurrence as soon as the corticosteroid doses were reduced. Upon resuming the interview with the patient’ s mother, clinical symptoms began at the age of 9 years old by the occurrence of a voluminous cutaneous erythema following multiple tick bites. One month later the patient presented with subacute lymphocytic meningitis, which was treated as tuberculosis meningitis without relevant bacteriological evidence. On the same day, the patient's two sisters were also bitten by ticks. A few weeks later on, one of them developed osteomyelitis of the tibia, which was operated on. The bacteriological examination result was negative, the histological examination showed non-specific osteomyelitis. Subsequently, she developed various multiple functional complaints that led to the diagnosis of fibromyalgia. Her other sister concomitantly developed seronegative rheumatoid arthritis. The diagnosis of chronic post-tick bite syndrome was suspected despite the negativity of the Lyme serology. The patient significantly improved thanks to high-dose of intravenous penicillin G, despite an initial exacerbation during the first month of treatment, which could be interpreted as confirmation of an infectious process (Jarisch-Herxheimer reaction). Corticosteroids and colchicine were discontinued ; the patient was given doxycycline for a total duration of 18 months. Antibiotic therapy was associated with hydroxychloroquine, the same way as reported for therapy of chronic Q fever and some types of Lyme disease . In 2004, the patient was in complete remission of Behçet's disease. Ten years later, no recurrence has been observed.
Archives of Microbiology & Immunology, Volume 05, pp 214-231; doi:10.26502/ami.93650059
Background: Low-grade glioma (LGG) can behave aggressively, akin to glioblastoma, and prognostic classification is urgently needed. N6-methyladenosine (m6A) modification is a key regulator of transcriptional expression during tumorigenesis and progression. This study aimed to identify transcriptome biomarkers with prognostic predictive value and define molecular subclassifications. Methods: We selected 21 m6A methylation-related genes for analysis of 529 LGG samples from TCGA LGG datasets and 1,152 brain tissues from the GTEx datasets. Through difference analysis, Protein-protein interactions (PPI) network, and spearman correlation analysis, gene expression and correlation were studied. Consensus cluster, gene ontology (GO) analysis, Kyoto Encyclopedia of Genes, and Genomes (KEGG) analysis were performed for classification and functional analysis. Lasso Cox regression algorithm and univariate and multivariate analyses were used for assessing risk factors. Results: The expression of m6A methylation-related genes between normal brain and LGG samples was significantly different. Consensus cluster analysis clearly divided LGG samples into two categories, with a p-value for the difference between prognosis close to 0. Through the lasso Cox regression algorithm and univariate and multivariate analyses, four genetic biomarkers (IGF2BP2, IGF2BP3, YTHDC1, and ALKBH3) were screened out, and the cumulative analysis of these effectively predicted patients’ prognosis. Conclusion: Consensus cluster analysis based on m6A methylation-related genes clearly divided LGG samples into two categories. Moreover, the cumulative analysis of four genetic biomarkers (IGF2BP2, IGF2BP3, YTHDC1, and ALKBH3) effectively predicted prognosis.
Archives of Microbiology & Immunology, Volume 05, pp 207-213; doi:10.26502/ami.93650058
Background: Globally, tuberculosis (TB) is the leading cause of death among People Living with HIV/AIDS (PLWHA). Nigeria has one of the largest burdens of TB and HIV/AIDS in the world. Most cases of TB in Nigeria are closely linked to HIV infections. Aim: In this study, the prevalence of TB among PLWHA was investigated. Methodology: One hundred and sixty three (163) sputum specimens were collected from patients attending COCIN Rehabilitation Center in Mangu Jos, Plateau State. The sputum specimens were stained by ZiehlNeelsen’s technique and examined microscopically for Acid Fast Bacilli (AFB). Results: Out of the 163 sputum specimens tested six were positive for AFB giving an overall prevalence of 3.7%. The prevalence of TB/HIV co-infection with respect to age group and gender revealed that patient within the age range of < 20 years had the highest prevalence of 14.3%, while male patients were found to have a prevalence of 4.9% compared to their female counterparts (1.7%). Regarding marital status, higher prevalence (9.1%) was recorded among patients who are married compared to the prevalence of 2.3% found among the singles. The prevalence in relation to occupation, showed that patients who engaged in farming had the highest prevalence of 5.2%, followed by those who identified as student (5.0%) and business (2.9%). However, no case of TB was recorded among patients who identified as civil servants and housewives. Conclusion: This study has established the link between TB and HIV infection in the study area. We therefore recommend that further studies be conducted in the entire Plateau State for possible intervention in terms of improve antiretroviral treatment coverage tuberculosis screening, treatment and prevention as a way of reducing active TB among people living with HIV.
Archives of Microbiology & Immunology, Volume 05, pp 232-242; doi:10.26502/ami.93650060
COVID-19 virus defined as illness caused by a novel corona virus which first discovered in Wuhan City, China. And On January 30, 2020, the WHO awarded the global health emergency. This is a review of COVID-19's highly affects on almost all the organs and how we precaution and management as the COVID-19 causes many systemic abnormalities like inflammation, endotheliitis, vasoconstriction, hypercoagulability, edema and Lymphocytopenia, with elevated D-dimer, and disseminated intravascular coagulation. Deep vein thrombosis and venous, thrombosis with pulmonary embolization, systemic and pulmonary arterial thrombosis, embolism are reported, ischemic stroke changes, and myocardial infarction are reported also. It can lead to acute coronary syndrome, with heart failure and myocarditis, arrhythmias. Kidney affection was usually secondary to systemic allover disturbances. Stroke may occurred. Delirium and seizures symptoms are common. Impaired the tastes are reported with Psychological disturbances are commonly, Lactate dehydrogenase may be elevated. Many skin manifestations including patchy erythematous rash are noticeable, One of the biggest barriers standing in the way of ending the pandemic the misinformation about the COVID-19 vaccines. At this critical time the Demand for vaccines very effective as it rigorously tested and found to be safe, also Vaccines have no effect on recipients’ genetic material and not effect on fertilization also Antibodies from are estimated to last two to four months, so those who have had a previous infection still get this vaccinated.
Archives of Microbiology & Immunology, Volume 05, pp 182-206; doi:10.26502/ami.93650057
Ebola viral disease remains a great threat to the society due to the limited availability of licensed drugs or vaccines. As the pathogen belongs to Biosafety Level 4, it is extremely dangerous to design a vaccine using conventional techniques. Therefore, reverse vaccinology approach is used to select the suitable epitopes from the genomic sequence of the virus. Using several algorithms, specific epitopes were chosen and 3D structures were obtained. The HLA alleles vary in different population and hence those alleles that commonly occur in African and world population were selected. Homology models were built for those HLA alleles whose 3D structures were unavailable. Then selected 20 epitopes were docked with selected 8 HLAs and further, selected epitope NQDGLICGL was validated by molecular dynamics study.
Archives of Microbiology & Immunology, Volume 5, pp 154-175; doi:10.26502/ami.93650055
Objective: To analyze the impact of study selection on the results of a recently published meta-analysis of the efficacy of hydroxychloroquine (HCQ) and hydroxychloroquine plus azithromycin (AZI) in Covid-19 patients. Methods: 31 studies were reviewed looking for critical bias. Combined hazard ratios and confidence intervals were calculated for both treatments using a fixed effects size model and a random effects model. Quantitative analysis regarding the toxicity of the association HCQ plus AZI is made. Results: Meta-analyses performed on the 11 studies we deem critically unbiased show a mortality reduction of 55% for HCQ and 66% for HCQ plus AZI. For both treatments, our meta-analysis indicates a significant efficacy in reducing mortality in hospitalized Covid-19 patients.
Archives of Microbiology & Immunology, Volume 4, pp 95-109; doi:10.26502/ami.93650049
Objective In this retrospective case study, we determine the results, side effects and long-term outcome features of treatment with imiquimod 5% in women with recurrent or residual cervical intraepithelial neoplasia (rrCIN). Methods The study was set in three outpatient clinics of hospitals in the Netherlands. Women diagnosed with rrCIN and treated with imiquimod 5% intravaginally between 2010 and up to and including 2017 were included. Data were extracted from medical records. The main outcome measures were complete regression or partial regression of SIL (squamous intraepithelial lesions) (cytology) or CIN (histology), side effects and long-term outcome in terms of the need for further excisional treatment during the follow-up period. Outcomes were assessed using descriptive statistics. Results The 18 studied women tolerated imiquimod well: all completed the treatment, with a temporary stop or dose reduction in two. The treatment was successful in 11 women overall (61%) of whom 8 women (80%) with high grade CIN (grades II-III). Of these 11 women, 4 women developed a recurrence of which 3 women were treated successfully with imiquimod or a LLETZ procedure. One woman died in the follow up without treatment of the CIN lesion. Of the remaining seven women with unsuccessful treatment, four patients underwent additional therapy. Two women underwent a hysterectomy, one woman underwent multiple procedures and the last woman underwent laser therapy and imiquimod, but died in the follow up. Of the remaining three out of those 7 women with unsuccessful treatment, two women refused further therapy and one woman required no further therapy since she cleared HPV and showed no dysplasia in the follow-up period. Conclusions In studied population, imiquimod treatment was well tolerated and associated with resolution or regression of SIL/CIN in 61% of women. We have started a randomized controlled trial to compare the efficacy of imiquimod with that of LLETZ in recurrent or residual CIN.
Archives of Microbiology & Immunology, Volume 4, pp 38-50; doi:10.26502/ami.93650043
In Oman, mastitis is an important disease that affects the dairy animals, especially cows. In this study, bacteria and fungi from subclinical and clinical mastitis were identified using 16S rDNA and 18S rDNA, respectively, in 76 milk samples from 30 cows. The frequency of subclinical mastitis (75%) was higher than clinical mastitis (25%). Bacterial isolates were detected in 82% of the samples, out of which 12% showed mixed bacterial cultures. The most predominant isolated bacteria were environmental bacteria rather than minor and contagious bacteria from subclinical (53.6%, 42.8% and 3.6%, respectively) and clinical mastitis (62.5%, 25% and 12.5%, respectively). Antibiotic resistance profiles of the isolated bacteria for six commonly used antibiotics showed an increase in resistance compared to a previous study in 1991. Most isolated bacteria were resistance to AMP, while they were more sensitive for SXT and TE. Eleven percent of the isolated bacteria were resistance to four of the antibiotics tested or more. About half of the samples (47%) were positive for fungal growth. Most of those samples were positive for bacteria, which suggested that detected fungi may be opportunistic. However, 3% of the investigated samples were negative for bacterial growth, which may indicate pathogenic involvement in mastitis. In conclusion, the major association of mastitis with environmental bacteria and the detected multi-antibiotics resistance emphasized the need for using appropriate control protocols by allowing to investigate each case and determine whether antibiotic treatment is necessary and which antibiotics to be used.
Archives of Microbiology & Immunology, Volume 4; doi:10.26502/ami.93650052
Archives of Microbiology & Immunology, Volume 4; doi:10.26502/ami.93650046