Türk Mikrobiyoloji Cemiyeti Dergisi

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ISSN : 0258-2171
Total articles ≅ 283
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Seher Akkus, Merve Cihan, Rüveyda Akçin, Dogukan Ozbey, Harika Öykü Dinç, Tevhide Ziver, Nesrin Gareayaghi, Serhat Sirekbasan, Suat Sarıbaş, Mert Ahmet Kuskucu, et al.
Türk Mikrobiyoloji Cemiyeti Dergisi, Volume 51, pp 263-270; https://doi.org/10.5222/tmcd.2020.50570

Kübra Evren, Hale Ahsen Yardibi Demir, Fatma Mutlu Sarıgüzel, Bedia Dinç
Türk Mikrobiyoloji Cemiyeti Dergisi, Volume 51, pp 239-244; https://doi.org/10.5222/tmcd.2021.71601

Ali Ahmet Kilimcioğlu, Nogay Girginkardeşler, Tuba Oyur, Selin Bölük Sabuncu, Didem Düzyol Azak, Serhan Görgün, Işın Akyar, Özgür Kurt, Tanıl Kocagöz, Ahmet Özbilgin
Türk Mikrobiyoloji Cemiyeti Dergisi; https://doi.org/10.5222/tmcd.2021.20981

Abstract:
Objective: It was aimed to develop a new Multiplex Polymerase Chain Reaction (PCR) protocol with isolates obtained from local patients for the diagnosis of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis, which can cause severe gastrointestinal system complaints especially in immunocompromised patients and children. Method: DNA isolation was performed with a commercial kit from three stool samples of different patients whose microscopic examination showed dense amounts of Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis. First, a special PCR protocol has been developed for each protozoon. Then, the multiplex PCR protocol, in which these three protozoa can be diagnosed together, was optimized. Results: In the multiplex PCR protocol performed after DNA isolation, bands of 95 bp., 227 bp. and 258 bp. were obtained for Cryptosporidium sp., Blastocystis sp. and G. intestinalis, respectively. Conclusion: Blastocystis sp., Cryptosporidium sp. and Giardia intestinalis were diagnosed by multiplex PCR with the original protocol developed. Due to the difficulties in using different methods in parasitological examination, by adding other protozoa important for public health to this optimized protocol, it will be possible to detect a large number of parasites with a single molecular method.
Hasan Cenk Mirza, Banu Sancak
Türk Mikrobiyoloji Cemiyeti Dergisi; https://doi.org/10.5222/tmcd.2021.96658

Abstract:
Objective: Members of Enterobacterales can cause various diseases in humans. The objective of this study was to determine the genus/species distribution and antimicrobial susceptibilities of Enterobacterales isolated from blood cultures in Central Laboratory of Hacettepe University Hospital. Method: Enterobacterales isolated from blood between July-2014 and April-2018 were included in the study. MALDI-TOF MS was used for the identification of isolates. Antimicrobial susceptibilities were determined with automated system (VITEK 2 Compact for the isolates between 2014 and 2018; BD Phoenix for the isolates in 2018) and disk diffusion method. Results of antimicrobial susceptibility testing were interpreted according to EUCAST breakpoints. Results: In total, 1765 isolates belonging to the order Enterobacterales were isolated from blood cultures. The most common microorganisms were Escherichia coli (47.6%), Klebsiella (34.1%), Enterobacter (6%), Proteus (4.4%) and Serratia spp. (3.5%), respectively. The remaining isolates included Salmonella, Citrobacter, M. morganii, Pantoea, Raoultella and Providencia spp. The lowest resistance rates among E. coli, Klebsiella and Enterobacter spp. isolates were observed against meropenem and amikacin. However, 21.1% of Klebsiella isolates were resistant to meropenem. The most active antimicrobials against Proteus isolates were piperacillintazobactam and meropenem. Resistance was not observed against piperacillin-tazobactam and meropenem among Proteus isolates. The most active antimicrobial against Serratia isolates was trimethoprim/ sulfamethoxazole with a resistance rate of 0%. Resistance was not noted against ampicillin and trimethoprim/ sulfamethoxazole among Salmonella isolates, whereas 26.1% of isolates were resistant to ciprofloxacin. All Citrobacter isolates were susceptible to meropenem, amikacin and cefepime. Conclusion: Findings of our study may guide the selection of proper antimicrobials for the treatment of bacteremia caused by Enterobacterales. Furthermore, this study provides important epidemiological information regarding the distribution of members of Enterobacterales causing bacteremia.
Ayşe Sultan Karakoyun
Türk Mikrobiyoloji Cemiyeti Dergisi; https://doi.org/10.5222/tmcd.2021.71602

Abstract:
Candida vaginitis (CV) is a neglected but growing public health problem. It is estimated that three out of four women have had at least one CV attack. The diagnosis and treatment of CV is often inadequate due to the tendency of women to self-diagnose and use over-the-counter drugs when they have vaginal or vulvar complaints, and clinicians plan treatment only according to clinical findings. There are limitations regarding the safety and efficacy of oral azoles, which are primarily preferred for the treatment of vaginitis, and these drugs have not revealed the desired levels of clinical or mycological cure. Also, there are a few options for current drugs used for treatment are not numerous the development of new antifungal drugs is thus urgently needed. Ibrexafungerp (IBX) is a semisynthetic triterpenoid glucan synthase inhibitor derived from enfumafungin. IBX has been shown to be a promising oral antifungal in the treatment of acute CV, and its use was approved on June 1, 2021. IBX is remarkable by it’s high oral bioavailability, low risk of side effects, few drug-drug interactions, good tissue penetration, increased activity at low pH in the vagina, and efficacy with regard to multi-drug-resistant fungi. In this review, in vitro and in vivo data on IBX were evaluated and compiled in light of current knowledge.
Gökçe Kader Aslan, Fatma Esenkaya Taşbent, Metin Doğan
Türk Mikrobiyoloji Cemiyeti Dergisi; https://doi.org/10.5222/tmcd.2021.26817

Abstract:
Objective: Stenotrophomonas maltophilia is an opportunistic pathogen that frequently causes nosocomial infections in recent years. It is generally isolated from respiratory tract samples, blood, urine and drainage materials. Due to multiple antibiotic resistance, a limited number of antibiotics are used in the treatment of these infections. The aim of this study is to investigate the antibiotic resistance status and risk factors in isolated S. maltophilia strains. Method: Diversity and antibiotic susceptibility levels of S. maltophilia strains isolated from various clinical samples between January 2018 and June 2020 were examined using conventional methods and VITEK2 automated system. Demographic and diagnostic data of the patients were retrieved from the hospital’s data base to identify the risk factors of infection. Results: Of the 300 strains examined, 46% were isolated from intensive care units, 35.3% from patients hospitalized in other clinics, and 18.7% from outpatient clinic patients. It was observed that 64 (21.3%) of 300 patients were immunosuppressed. Trimethoprim-sulfamethoxazole resistance was 1.3% and levofloxacin resistance was 0.7%. Conclusion: Resistance rates were found to be lower than the literature data in the study. It was concluded that hospitalization in the intensive care unit and immunosuppression are important risk factors for S. maltophilia infections.
Rahime Aksoy, Ebru Us
Türk Mikrobiyoloji Cemiyeti Dergisi; https://doi.org/10.5222/tmcd.2021.20591

Abstract:
Objective: The presence of anti-nuclear antibodies (ANA) are important indicators of systemic connective tissue diseases. Anti-DFS70 autoantibody is also known to be found in healthy individuals and has not yet been identified in relation with diseases. The aim of our study was to investigate the relationship between clinical complaints, diagnoses and inflammation parameters [C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and rheumatoid factor (RF)] of patients with anti-DFS70 antibody positivity detected by Indirect Immune Fluorescent-ANA (IIFANA) and Immunoblot method. Method: A total of 450 patients, 412 (91.5%) women and 38 (8.5%) men, between the ages of 4–90, were included in the study. The clinical diagnoses and inflammation parameters of the patients were evaluated retrospectively. Results: Complaints were pain (126/136) and rash, dyspnea, pruritus (10/136). While inflammation was not detected in 81 (64.3%) of the patients with pain complaints, it was found in 45 (35.7%) and was found to be statistically significant (p=0.001). Inflammation was found to be negative in 106 (67.9%) patients with systemic involvement of connective tissue, positive in 50 (32.1%) patients (p<0.001), negative in 19 (95%) and positive in 1 (5%) patients with urticaria (p<0.001). Ten of the 60 patients with a definitive diagnosis had systemic involvement of the connective tissue, 10 had urticaria, and 40 had different diagnoses. Conclusion: It was concluded that DFS70 positivity was higher in women and this pattern may accompany many diseases. Our study suggests that the cause of pain may be the DFS70 antibody when inflammation parameters are eliminated, and that there may be a relationship between urticaria and DFS70 autoantibodies. Keywords: Anti-nuclear antibodies, DFS
Banu Hümeyra Keskin, Emel Çalışkan, Sare Kaya, Ezgi Köse, Idris Şahin
Türk Mikrobiyoloji Cemiyeti Dergisi, Volume 51, pp 254-262; https://doi.org/10.5222/tmcd.2021.82787

Mayram Hacıoğlu, Özlem Oyardı
Türk Mikrobiyoloji Cemiyeti Dergisi; https://doi.org/10.5222/tmcd.2021.59023

Abstract:
Objective: Polymicrobial biofilms consisting of a combination of various bacteria and/or fungi are generally much more resistant than monomicrobial biofilms formed by these species alone. In this study, it was aimed to investigate how Candida albicans biofilms were affected in the presence of Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii or Pseudomonas aeruginosa or supernatants of these bacteria. Method: C. albicans with Gram-negative polymicrobial biofilms were formed on sterile microplates by using control strains of C. albicans SC 5314, E. coli ATCC 25922, K. pneumoniae ATCC 700603, A. baumannii ATCC 19606 and P. aeruginosa PA01. The number of C. albicans in biofilms was determined in the presence of both Gram- negative bacteria and sterile supernatants. Results: According to our results, all Gram negative bacteria displayed an antagonist effect against C. albicans in the biofilm and a three log decrease was observed compared to the control. Sterile supernatants were shown to have an inhibitory effect on the C. albicans biofilms and reduce the number of yeasts by at least one log. MTT assay and fluorescence microscopy images also confirmed the results. Conclusion: In C. albicans-Gram-negative polymicrobial biofilms that can occur in many infections, bacteria affected C. albicans biofilm cells as antagonist agents, both with their cells and sterile cell-free supernatants
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