Objective: To explore the effects of different micrononics on biological characteristics and variants of Lycoris chinensis, lay the foundation for their domesticated cultivation, resource protection and sustainable development and utilization. Method: Through the sample investigation, statistical analysis of the structure, advantage species, ornamental plant diversity and Lycoris chinensis in different Lycoris chinensis; And compare different habitats, Microbial Biological Characteristics of wild Group of Wild Groups in Lycoris chinensis in Different Habulous. Result: The population structure of wild Lycoris chinensis in different micronemia is arbor + shrub + Herbs; the plant community 45 species of 45 species, arbor is built into Celtis sinensis, Liquidambar formosana; the shrub floor is built into a Camellia japonica, Rhododendron simsii Planch.; herbal layer is built into Pellionia radicans, Centella asiatica, Lycoris chinensis. There are few types of trees in the plant community, and the species in the herb are relatively rich. Overall, Herbal layer > Treble > Shrub. There is a significant difference between the sample, the overall performance of light and slope in the micronon birth; the overall performance of China’s stone garlic is D3 > D1 > D4 > D2. Conclusion: Lycoris chinensis is a very development and potential and has a very strong ornamental plant, and different micronon birth conditions the identification form and population structure of Lycoris chinensis is different.

In order to participate in the different pictures of the phosphorus-dissolving and potassium-dissolving compound bacteria and the visual effects of black grass animals, phosphate-dissolving bacteria and gum-dissolving bacteria were analyzed from the rhizosphere line of phosphorus and potassium along the wheat-Qinghai-Tibet highway. The effects of supplementary supplements are the best types, namely: Bacillus argentii (P09, phosphate solubilizing bacteria); Enterobacter roggenkampii (K02, resolving canister bacteria); Bacillus spores (P09, solution Phosphorus bacteria) K04, canning bacteria). The strains were prepared into different proportions of bacterial agents, which were 1:0:0, 0:1:0, 0:0:1, 1:1:0, 1:0:1, 0:1:1, 1:1:1, 1:2:1, 1:1:2 and 2:1:1. The compound inoculum was applied to the soil of alfalfa and ryegrass. By measuring various physiological indicators of plants, the optimal ratio of compound microbial agents is finally determined. After the application of bacterial fertilizer, all the compound bacterial agents have a growth-promoting effect on alfalfa and ryegrass. The overall growth-promoting effect of the 1:1:0 treatment is more obvious than other treatments. The plant height and leaf diameter of alfalfa and ryegrass plants (Wide), tissue water content, chlorophyll content, soluble sugar content, and peroxidase activity increased by 15.33%, 9.00%, 3.35%, 78.19%, 40.11%, 40.77% and 35.55%, 18.60%, 2.47%, 33.53%, 50.00%, 42.66% compared with CK, respectively. Experiments show that the 1:1:0 ratio of bacterial agents has the most obvious growth-promoting effect on plants. Therefore, P09 strain and K02 strain have obvious promoting effect on the growth of plants, which can help plants absorb phosphorus and potassium. Appropriate application of this ratio of inoculants in the soil will help the growth of alfalfa, ryegrass and other plants. Improving the ecological system along the Qinghai-Tibet Highway will help.

The extraction and determination of the main active ingredient from the seeds of “Guiyou No.1”, a new variety of Shatian pomelo as the testing piece, were carried out. The antidiabetic effect of sodium glucose cotransporter 2 (SGLT-2) of the active ingredient, an important target of diabetes mellitus, was studied. The results showed that limonin, naringin and neohesperidin were the main active ingredient of “Guiyou No.1” seed. Among them, neohesperidin and naringin showed relatively good inhibitory effect on SGLT-2 activity, and showed hypoglycemic effect in diabetic mouse model, the hypoglycemic rate was 31.29% and 26.32%, respectively. The results are significant for the comprehensive development and utilization of “Guiyou No.1”.

This paper investigates and analyzes the medicinal plant resources in Songpan County, Sichuan Province. The survey shows that: 1591 species belonging to 696 genera and 173 families of medicinal plants were collected, including 180 species belonging to 151 genera and 80 families of key medicinal plants, and 37 species belonging to 29 genera and 21 families of national key protected and rare medicinal plants. According to the investigation and analysis, some suggestions on the protection and utilization of medicinal plant resources were put forward.

To identify the pathogenic fungus that caused peanut southern blight in Jingzhou City of Hubei Province, the pathogens of peanut southern blight were isolated, purified and identified using the methods of tissue isolation, morphological identification, and molecular identification, which is based on ITS. The pathogenic fungi (QZ) that caused peanut southern blight were identified as Athelia rolfsii, which belonged to the subphylum basidiomycete fungus. The unnatural state is Sclerotium rolfsii Sacc., which belongs to the subphylum hemiknophyllum.

Wheat powdery mildew is a leaf disease that occurs during the whole growth period of wheat, which can significantly reduce the yield and quality of wheat. Planting disease-resistant varieties has always been regarded as the most economical, safe and effective way to control wheat diseases. In order to grasp the resistance level of main plant varieties, newly approved varieties and some high-generation lines to powdery mildew in the middle and lower reaches of the Yangtze River and the Huanghuai wheat area. In this study, the physiological race 52-27 of powdery mildew was used to identify the disease resistance at the seedling stage of the main varieties and reserve wheat vari-eties (lines) in the middle and lower reaches of the Yangtze River and the Huanghuai wheat region; the physiological races E21, 52-27 and 2-40 were used for disease resistance identification of 49 wheat varieties (lines). The results showed that among the 74 test varieties (lines) with resistance at the seedling stage, 25 were resistant to 52-27, accounting for 35.21%. Among the 49 cultivars (lines) identified by the in vitro leaf segment method, 13 were resistant to E21, accounting for 26.53%; 17 were resistant to 52-27, accounting for 34.69%; 12 were resistant to 2-40, accounting for 24.49%; 2 of the 3 races were resistant to disease, accounting for 4.08%.

In order to study the effects of exogenous tryptophan on the growth and physiological characteristics of tomato seedlings, different concentrations (0, 50, 100, 200, and 400 mg/L) of tryptophan solution were sprayed on the leaves. The results showed that the biomass and strong seeding indexes of tomato seedlings under 200 mg/L tryptophan treatment were greater than those of other treatments. With the increase of tryptophan concentration, the chlorophyll and carotenoid contents of leaves showed the trend of increasing first and then decreasing, reaching the maximum at 200 mg/L treatment. The tomato seedlings’ soluble sugar and soluble protein contents were significantly increased by exogenous tryptophan treatment, with the 200 mg/L tryptophan treatment having the highest value. The antioxidant enzyme activities of SOD and POD in tomato leaves of all treatments were increased first and then decreased, reaching the maximum at 200 mg/L treatment, and the lowest MDA content occurred in the 50 mg/L treatment. Taken together, our results showed that 200 mg/L exogenous tryptophan treatment exhibited the most benefits in improving tomato seedlings’ growth and physiological activity.

In order to expand the development space of forestry, improve the ecological environment, and improve the economic, ecological and social benefits of forestry in the middle and west of Jilin Province, this paper adopts the mixed afforestation model of Xanthoceras sorbifolia B. and Hippophae rhamnoides L., which has broad application prospects.

The signal transduction involved in ABA regulates various stages of plant growth and develop-ment, such as seed germination, stomatal closure, root development and senescence, and regu-lates plant adaptation to drought, cold, heat, salt and other stresses. The study of ABA signal transduction pathway is of great significance for understanding the regulation mechanism of plant growth and development. The results of RT-PCR showed that At2g34610 was an ABA-responsive gene and its expression was up-regulated by ABA. In order to study the function of At2g34610, gene multi-target editing vector and overexpression vector were constructed respectively. The vector construction laid a foundation for the creation of target gene overexpression and mutant plants, which are ideal experimental materials for exploring the role and molecular mechanism of At2g34610 in ABA signal transduction and regulation of stress resistance in Arabidopsis thaliana.

After investigating the species composition of basidiomycetes in Wuling Mountain Nature Reserve, the list of basidiomycetes was compiled. The survey results show that there are 168 species of 63 genera in 44 families in Wuling Mountain. Among them, red mushroom, Mushaceae, porous and Filanthaceae contained the largest number of species, accounting for 12.5%, 11.9%, 6.5% and 5.4% of the total number of species, respectively.