Jurnal Biologi Tropis
ISSN / EISSN : 1411-9587 / 2549-7863
Published by: Mataram University (10.29303)
Total articles ≅ 433
Latest articles in this journal
Jurnal Biologi Tropis, Volume 22, pp 461-470; https://doi.org/10.29303/jbt.v22i2.3351
Dafala village is one of the villages in NTT that uses lenglengan leaves as traditional medicine to treat diseases such as coughs, TBC, diarrhea and back pain.This present research aims to know the bioactive compounds and composition of the bioactive contained in Lenglengan (Leucas lavandulifolia Sm.) leaf extract, and too know the effectiveness of lenglengan leaf extract in inhibiting Staphyloccocus areus bacteria. We extracted leaves of Lenglengan by maceration method using ethanol. The extract was obtained and then tested in a qualitative way to determine the bioactive compounds are made by using reagents. While in the quantitative test to Determine of levels of flavonoids, phenols and tannins using UV-Vis spectrophotometry method, alkaloids and saponins using the gravimetric method. The Antibacterial test was carried out by disc paper method. Paper discs containing extract concentrations: 12.5%, 25%, 50%, 62.5% and 75%, positive control (gentamicin) and negative control (aquadest) were placed on MHA media containing S. aureus suspension, incubated at 37°C for 24 hours and then the diameter of the inhibition was observed. Qualititive test result show that the lenglengan leaf extract contains 5 bioactive compounds include alkaloids, flavonoids, saponins, tannins, and phenols. The composition of bioactive compounds in Lenglengan leaf extract: flavonoids 23.93%, saponins: 19.05%, alkaloids: 15.28%, tannins: 5.81%, and phenols: 2,335%. The atibacterial result reveal that lenglengan leaf extract was significantly affect in inhibiting Staphylococcus aureus. The higher concentration of extract lenglengan leaves used, the higher the inhibition zone produced. The highest level response inhibition showed from level of 12.5%: 11.3 mm, 25%: 12.4 mm, 50%: 13.43 mm, 62.5%: 14.43 mm and 75%: 15.58 mm, gentamicin: 17.4 mm aquades: 0 mm.
Jurnal Biologi Tropis, Volume 22, pp 455-460; https://doi.org/10.29303/jbt.v22i2.3356
Early identification is needed to predict the condition of patients who are at risk of worsening symptoms to become more severe. Simple laboratory tests such as measurement of the neutrophil-lymphocyte ratio are known to be used as factors to determine the patient's prognosis in various clinical situations. The purpose of this study was to analyze the value of the neutrophil-lymphocyte ratio as a predictor factor for COVID-19. This study is retrospective, using medical record data of 327 Covid-19 patients at the Dr Chasan Boesoirie Hospital Ternate from April to September 2021. Subjects were classified as not severe and severe COVID-19. The value of the neutrophil-lymphocyte ratio was determined based on the results of a routine blood test (sysmex XN-1000B3 BF). Data were analyzed using SPSS version 16, Pearson's test to determine the relationship between NLR and severity and using the ROC (Receiver Operating Characteristic) curve to determine the strength of the relationship with the Cut-off value, p < 0.05 and AUC to determine the sensitivity and specificity of NLR to COVID-19 severity. Results: From 327 subjects consisting of 229 non-severe and 98 severe, the neutrophil-lymphocyte ratio (NLR) in the non-severe (3.45±2.02) was significantly different from the severe (9.73±5.64) (p < 0.00). The ROC (Receiver operating Characteristic) curve shows that the NLR has a sensitivity of 83%, specificity of 82% with a cut-off of 3.5 with a positive predictive value (PPV) of 88.2% and a negative predictive value (NPV) of 83.6%. The results of this study can be used as a reference for determining clinical prognosis using neutrophil ratio data.
Jurnal Biologi Tropis, Volume 22, pp 353-359; https://doi.org/10.29303/jbt.v22i2.3240
Geographical conditions affect human physical and physiological conditions, one of the examples is the height of residence that can make the respiratory system different among population groups. This study aims to compare the respiratory system between highlanders and lowlanders in terms of hemoglobin levels, blood oxygen saturation, and breaths frequency. The research model used was a comparative study with a purposive sampling method based on: age criteria with a range of 16-19 years, a minimum of 6 months stayed at the research location, and there is no recorded respiratory system disease. Data analysis used was independent t-test with Shapiro-Wilk prerequisite test for normality and one-way ANOVA for homogeneity, then Mann-Whitney test for follow-up non-parametric test. The results showed that the highlanders generally have oxygen saturation and hemoglobin levels with an average value of 98.81% and 14.69g/dL higher than those in the lowlands with a value of 97.86% and 13.14g/dL. On the other hand, the average value of breath frequency of highlanders is 17.38/minute which tends to be lower than that of lowlanders with 24.17/minute. The prerequisite test showed highlanders oxygen saturation data that were not normally distributed with a significance value of 0.003, then oxygen saturation data and breath frequency were not homogeneous with a significance value of 0.000 and 0.003 respectively. The comparative test showed a significance value of 0.002 for hemoglobin through independent t-test, then obtained asymptomatic significance values of 0.004 and 0.000 for oxygen saturation and breaths frequency through the Mann-Whitney test. Based on the significance value obtained, it can be stated that there are significant differences in hemoglobin levels, oxygen saturation, and breaths frequency per minute between highland and lowland residents. It is recommended to conduct further research on lung capacity and chest cavity index to provide a more holistic explanation of the differences in the respiratory system between highland and lowland residents.
Jurnal Biologi Tropis, Volume 22, pp 434-440; https://doi.org/10.29303/jbt.v22i2.3367
Gunung Leuser National Park (TNGL) is one of Indonesia's conservation areas, serving as a haven for various plants and animals. The ecotourism zone is a part of the utilization zone in the GLNP area that is intended for the development of ecotourism and has a high diversity of plant species. However, information concerning the potential of plants in the GLNP's ecotourism zone has not been widely disseminated. The present study aims to inventory plant species in Gunung Leuser National Park's ecotourism zone. The exploration method was used in this study, which lasted from November to December 2021. A total of 47 plant species from 30 families were discovered in the study area, with Apocynaceae, Euphorbiaceae, Fabaceae, Lamiaceae, and Solanaceae having the most species. These plants have a variety of uses, including food, spices, ornamental plants, and materials for traditional/ritual ceremonies.
Jurnal Biologi Tropis, Volume 22, pp 485-493; https://doi.org/10.29303/jbt.v22i2.3446
Fern is the best growing medium for orchids, because it is protected, a solution is needed to find a substitute for fern media. In this study, a combination of stem charcoal, husk charcoal and peanut shell compost was chosen. The combination of the three was chosen because the charcoal sticks and husk charcoal are relatively sterile and the peanut shell compost can function as a medium and fertilizer. The purpose of this study 1. To determine the effect of the composition of the planting medium on the growth of Dendrobium sp orchid seeds 2. To determine the best composition of the growing media for the growth of Dendrobium sp orchid seeds. The research time was 2.5 months, the object of the research was the Dendrobium sp. orchid aged 12 months, the research location was at Paranet's house, Gabahan Village, RT 05 RW 12, Jombor Sukoharjo. Simple randomized study design with 4 treatments. Treatment A Planting orchid seeds with a mixture of ferns and peanut shell compost in 10 pots. Treatment B was planting orchid seeds with a mixture of stem charcoal, husk charcoal, and peanut shell compost in a ratio of 1:1:1 for 10 pots. Treatment C was planting orchid seeds with a mixture of charcoal stems, husk charcoal and peanut shell compost in a ratio of 1/4:1/4:1/2 for 10 pots. Treatment D was planting orchid seedlings. The variables observed were the number of leaves, leaf length, leaf area, number of tillers, and wet weight measured 12 weeks after treatment. Data analysis using Varieties Test (Anova or Kruskal Wallis Test) followed by Duncan's Multiple Range Test (DMRT) or Mood Median Test. Growth parameters were observed (leaf length, number of leaves, leaf area, and wet weight). As for the number of tillers, there was a difference in influence, although the difference was not significant. Treatment The combination of K.1/2 media or with a comparison of peanut shell compost, charcoal stems and husk charcoal 1/2:1/4:1/4 gave the best effect on the 4 observed growth parameters (Leaf Length, Leaf Number, Leaf Area). , and wet weight). From the results of this study, collectors, orchid lovers and orchid entrepreneurs can use a combination of stem charcoal, husk charcoal and peanut shell compost as a substitute for ferns with a mixture of charcoal stems, husk charcoal, and peanut shell compost in a ratio of 3/8:3/ 8:2/8.
Jurnal Biologi Tropis, Volume 22, pp 471-477; https://doi.org/10.29303/jbt.v22i2.3305
Hymenopteran order is an order of insects that is useful for pollinating plants, producing honey and wax and can kill insects that harm plants (pests). This study aims to determine the results of collections of Hymenoptera parasitoid insects from forest stands and rice fields. The research was carried out in January-March 2019. The sampling locations were carried out in two types of areas, namely forest stands and rice fields. Based on the results of the study, it was found that the two orders of insects that had a dominant number from the collection were the Order Diptera and Order Hymenoptera. The number of Hymenoptera insects in the forest stands and rice fields were 1.586 individuals and 2.179 individuals, respectively, with a total of 21 families of parasitoid insects. The use of separators in the collection of Hymenoptera parasitoid insects has several advantages compared to using other insect net, namely making it easier to collect insects and the number of insects caught is also higher.
Jurnal Biologi Tropis, Volume 22, pp 381-389; https://doi.org/10.29303/jbt.v22i2.3321
Polyethylene terephathalate (PET) is a type of plastic content that is commonly is difficult to degrade so that it has an impact on global environmental problems. Plastic waste pollution needs to be overcome by using environmentally friendly methods to accelerate the PET plastic biodegradation process. Biodegradation is the use of microorganism activity to decompose plastic compounds so as to reduce the volume of waste. Ideonella sakaiensis is a bacterium that produces PETase enzymes that play a role in degrading PET. This literature review aims to determine the potential of Ideonella sakaiensis encoding the PETase gene in degrading polyethylene terephathalate plastic waste by discussing three focus studies, namely: 1) the potential of Ideonella sakaiensis, 2) the characteristics of polyethylene terephathalate plastic waste, 3) the relationship between the PETase coding gene and the degradation of polyethylene plastic waste terephathalate. Biodegradation of plastic waste Polyethylene terephathalate using Ideonella sakaiensis which has the potential to degrade PET faster with the PETase enzyme. The biodegradation mechanism carried out involves the process of transforming the PETase coding gene from I. sakaiensis to Escherichiacoli bacteria influenced by pH and the effectiveness of the PETase enzyme work until the use of harvested products interacts with Polyethylene terephathalate plastic waste, which decomposes plastic waste. ÃÂ
Jurnal Biologi Tropis, Volume 22, pp 374-380; https://doi.org/10.29303/jbt.v22i2.3114
The location close to urban areas and the rapid rate of decline in area can threaten the diversity of rare fruits in the Sibohe Forest. A re-survey in the Sibohe Forest, Singkawang, West Kalimantan was conducted to confirm reports of the existence of 15 rare fruit plant species collected in 2017-2018 by previous researchers. The inventory process started from collection of specimens carried out during June-July 2021, herbarium making, identification and descriptive data analysis. Based on the similarity of regional names, 13 out of 15 species were confirmed according to the previous reports, while 2 species were not found due to the possibility of out of fruiting season. However, 4 of the 13 confirmed species were identified with different scientific names. Additional survey to the people in Pontianak City aged 17-34 years revealed some different names for the same type of fruit among these four species. Four new plant species were also collected and described in this study including amok (Alpinia sp., Zingiberaceae), nubik (Artocarpus sp., Moraceae), tehengan (Artocarpus sp., Moraceae), and smallest arok (Ficus sp., Moraceae). Potentials and challenges in the inventory of rare fruit plants in the Sibohe Forest should be studied regularly as well as efforts to increase public knowledge and interest, especially for the younger generation. Moreover, recommendation should be made to support conservation activities based on the actual data collected in the field regularly.
Jurnal Biologi Tropis, Volume 22, pp 511-517; https://doi.org/10.29303/jbt.v22i2.2972
Long bean (Vigna sinesis L.) is a horticultural plant that is easy to cultivate. Cultivation of long bean plants certainly has several factors that can reduce production yields such as environmental factors, pest attacks, and diseases. This study aims to determine the pests and diseases that generally attack long bean plants and the symptoms they cause. This study used direct observation method (Systematic sampling) to determine the percentage of pest and disease attacks on long bean plants. From the total number of plants in the location, 5 plant samples were taken to be observed. Based on the pest and disease identification research that has been carried out, it was found that some of the symptoms that arise due to pest/disease attacks on most of the long bean plants were found. Symptoms that appear include damage to flower buds and leaves with holes caused by black ladybugs (Brachyplatys sp.); leaf curl, leaves and stems turn black due to aphids (Aphis craccivora); jagged leaf edges, and holes caused by grasshoppers (Oxya sp.) and lasiocampidae caterpillars; thickening of the leaf veins on young leaves, leaves shrinking caused by Bean Common Mosaic Virus (BCMV); The leaves turn yellow and the bone blanching occurs due to the Mungbean Yellow Mosaic Virus (MYMV).
Jurnal Biologi Tropis, Volume 22, pp 504-510; https://doi.org/10.29303/jbt.v22i2.3447
Kale land is a short-lived plant, containing the nutrients needed by the human body. The growth of kale land is influenced by various factors, one of which is the adequate supply of nutrients needed by plants. Fertilization is one of the efforts that can be done to ensure the availability of nutrients on agricultural land. Research has been carried out on the growth response of kale land due to the application of vermicompost and NPK fertilizers. The purpose of this research is to obtain information about (1) increased growth of kale land due to the application of vermicompost, (2) increased growth of kale land due to application of NPK fertilizer, (3) increased growth of kale land due to the interaction of vermicompost and NPK fertilizer treatments. The research was carried out using an experimental method in the form of factorial arranged according to a completely randomized design. The first factor is vermicompost (C) with doses of 0 kg, 0.4 kg, 0.8 kg, 1.2 kg and 1.6 kg vermicompost per square meter of agricultural land. The second factor is the application of NPK fertilizer (P) at a dose of 0 g, 0.5 g, 1 g, 1.5 g per plant. Thus there were 20 treatment combinations and each combination was carried out with 3 replications. The growth parameters observed were plant height, total leaf, leaf length, leaf width and stem diameter of land kale. Research data were analyzed by Anova. The conclusions of the study were: (1) The application of vermicompost could increase plant height, number of leaves, leaf length and leaf width but could not increase stem diameter of kale land (2) application of NPK fertilizer can improve all growth parameters of kale land kangkong. (3) The interaction of vermicompost and NPK fertilizer cannot increase the growth of kale land.