Refine Search

New Search

Results in Journal Journal of Stem Cell Therapy and Transplantation: 24

(searched for: journal_id:(59895))
Page of 1
Articles per Page
by
Show export options
  Select all
, Ramesh Geetha, Basha Sabiha Hayath, Kannan Ta, Hussain Shahid
Journal of Stem Cell Therapy and Transplantation, Volume 6, pp 001-004; https://doi.org/10.29328/journal.jsctt.1001024

Abstract:
In the present study, omental adipose tissue was collected from, the animals that underwent ovariectomy and ovariohysterectomy, surgical procedures, at the age of seven months to 11/2 years of age groups. The sample was digested with 0.1% (W/V) collagenase type I and transferred to a beaker with a magnetic stirrer and kept in a stirrer with 600 rpm at 37 °C for 30 minutes. The viability of the cell was evaluated by the trypan blue exclusion method using a hemocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the member in a dead cell and dye it blue. In the present study, the cell yield of fAD-MSCs was 8.15 ± 0.68, 6.55 ± 0.26, 4.85 ± 0.42, 3.90 ± 0.34, and 3.51 ± 0.43 in different age groups viz., 7,8,9 month 1 and 1½ year respectively. In younger age groups, cell yield and viability percentage were more than in animals of higher age groups. In the younger age group, stem cells proliferation status is considered potent for therapeutic application.
, Samy Romysa, Beshoy Sf Khalil, Khalil Beshoy Sf, Kordy Aya Ahmed, Said Marwa Mh, Rashed Nouran S, Fakruzzamana, Ik Kong
Journal of Stem Cell Therapy and Transplantation, Volume 5, pp 001-003; https://doi.org/10.29328/journal.jsctt.1001023

Abstract:
The oocyte is the female gamete that contributes not only half of the genetic material but also all of the cytoplasm to the zygote, supplying the transcripts, proteins, mitochondria and other components necessary for early embryonic development. The intrinsic oocyte quality is one of the main factors affecting the embryo yield, the implantation rate and the rate of healthy offspring. It is obvious that a fertilized oocyte must reach the blastocyst stage within 6–9 days in the proper culture conditions to have a significant chance of inducing a pregnancy and producing an offspring. The ability to sustain the first week of embryonic development is clearly influenced by the follicular status from which the oocyte is obtained indicating that this developmental potential is inherent within certain oocytes. Since most early embryos that do not reach the blastocyst stage are blocked at or close to the maternal to zygotic transition (MZT)-stage, which occurs at the eight-cell stage in cattle, one could speculate that incompetent oocytes fail to appropriately activate the embryonic genome. Oocyte selection based on glucose-6-phosphate dehydrogenase (G6PDH) activity has been successfully used to differentiate between competent and incompetent bovine oocytes. Recently, molecular regulation of genes regulating biological process of Brilliant Cresyl Blue staining (BCB) selected oocytes and embryos was investigated to explain their variation in quality and developmental potentiality. This short review will highlights some of these efforts that have been done in this interesting area of research.
Bawazir Amen, Al Kabli Reem, Bukhari Abdullah, Khader Esraá, Alrashed Mohammed, Alzahrani Mohammed, Al Badri Motasim
Journal of Stem Cell Therapy and Transplantation, Volume 4, pp 017-021; https://doi.org/10.29328/journal.jsctt.1001022

Abstract:
Objectives: The aim of this study was to assess the knowledge, attitude and motivation toward stem cell donation among Saudi population in Riyadh, Saudi Arabia. Methods: This is a cross-sectional study that was conducted at different malls in Riyadh. Selection of malls was done randomly according to the geographical distribution of Riyadh, in which sample size was calculated and distributed equally. The participants were asked to complete a questionnaire that addressed their knowledge, attitude and motivation toward stem cell transplantation and donation. Results: Results of this study showed that population knowledge about stem cell transplantation and donation is considered to be low. Only (37.8%) has enough information about stem cell transplantation and donation. There is a positive correlation between level of education and participant’s knowledge regarding stem cell transplantation and donation. The study revealed that 39.3% of participants have willingness for stem cell donation. Conclusion: It has been found that two third of population expressed lack of knowledge about stem cell transplantation and donation. Also, only 40% of participants showed willingness for donation, and the most common reason for not donating stem cell was the lack of information about stem cell and the value of donation While, increasing level of education was associated with better understanding of stem cell donation and its role in therapy and saving lives. Therefore, suitable campaign, advertising and counseling program for population is recommended to increase level of knowledge and motivation toward stem cell donation.
Benetti Federico, Natalia Scialacomo, Enrique Mariani, Luis Geffner, Bruno Benetti Eng, Daniel Brusich, Yan Duarte, Ernesto Peñaherrera
Journal of Stem Cell Therapy and Transplantation, Volume 1, pp 017-027; https://doi.org/10.29328/journal.jsctt.1001003

, Melissa Popowski, Bum-Kyu Lee, Cathy Rhee, Vishwanath R Iyer
Journal of Stem Cell Therapy and Transplantation, Volume 1, pp 052-062; https://doi.org/10.29328/journal.jsctt.1001005

Abstract:
Research into regulation of the differentiation of stem cells is critical to understanding early developmental decisions and later development growth. The transcription factor ARID3A previously was shown to be critical for trophectoderm and hematopoetic development. Expression of ARID3A increases during embryonic differentiation, but the underlying reason remained unclear. Here we show that Arid3a null embryonic stem (ES) cells maintain an undifferentiated gene expression pattern and form teratomas in immune-compromised mice. However, Arid3a null ES cells differentiated in vitro into embryoid bodies (EBs) significantly faster than control ES cells, and the majority forming large cystic embryoid EBs. Analysis of gene expression during this transition indicated that Arid3a nulls differentiated spontaneously into mesoderm and neuroectoderm lineages. While young ARID3A-deficient mice showed no gross tissue morphology, proliferative and structural abnormalities were observed in the kidneys of older null mice. Together these data suggest that ARID3A is not only required hematopoiesis, but is critical for early mesoderm differentiation.
Page of 1
Articles per Page
by
Show export options
  Select all
Back to Top Top