DENTA, Volume 12; doi:10.30649/denta.v12i2.170
Background: Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. Purpose : To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. Material and Method : The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). Results : There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). Conclusion : The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.
DENTA, Volume 12; doi:10.30649/denta.v12i2.175
Backgrounds : Enterococcus faecalis is a facultative anaerobic gram-positive bacteria that known to have a role in the infection of the root canal, causing the failure of root canal treatment. The extract from anchovy (Stolephorus insularis) contains fluor has antibacterial properties that can inhibit the growth of gram-positive bacteria and has the potential to be developed as a root canal sterilization agent. Purpose : To determine the antibacterial effect of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. Methods : This study was an true experimental study with post test only control group design and were tested by diffusion methods, consisted of 5 groups: 2 control groups DMSO 1% as negative control and ChKM as positive control, 3 treatment groups of anchovy (Stolephorus insularis) extract with different concentrations of 18%, 24% and 30% which each group consisted of 5 samples. Antivbacterial effect was examined by measuring the clear zone around the filter paper. Data were analyzed by ANOVA test followed by LSD test. Results : Results of this study proved the presence of antibacterial effects of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. In this study, the higher concentrations used, the greater the antibacterial effect. Average inhibition zone of each groups: DMSO 1% (6,03 mm), ChKM (14,18 mm), 18%(6,83 mm), 24%(7,03 mm) and 30%(7,16 mm). Conclusion : Anchovy (Stolephorus insularis) extract has antibacterial effect to the Enterococcus faecalis.
DENTA, Volume 12; doi:10.30649/denta.v12i2.161
Background: Candidiasis is one of oral infectious disease caused by Candida albicans.The role of C. albicans as pathogen opportunistic in oral infection can affected by immune system. Phagocytosis has contributed in immune system against C. albicans infection, which is the role of monocytes. Grapthophyllum L. Griff has been proved to increase macrophage’s phagocytic activity, but the effect of violet leaves ethanol extract (EEDU) to increase monocyte’s phagocytic activity on C. albicans is unknown. Objectives: To determine the effect of violet leaves extract on monocyte’s phagocytic activity on C. albicans. Methods: This research was acted in vitro on human monocyte cell culture. There was 5 groups: negative control group, positive control group (incubated in Isoprinosine) and treatment groups was incubated in EEDU 2,5%, 5%, 10%. The percentage of activated monocytes was counted. Result: The result showed that incubation of EEDU 2,5%, 5% and 10% could increase monocyte’s phagocytic activity significantly (p<0,05) compared to control groups. The number of monocyte’s phagocytosis on C. albicans incubated with EEDU 2,5%, 5% and 10% is 65%, 60,5%, and 58,75%. The optimal concentration of EEDU to increase monocyte’s phagocytic activity was 2,5%. Conclusion: The study showed that EEDU can increase monocyte’s phagocytic activity on C. albicans. Keywords:Ethanol extract of Graptophyllum pictum L. Griff leaves, Monocyte’s Phagocytic Activity, Candida albicans
DENTA, Volume 12; doi:10.30649/denta.v12i2.174
Background: Porphyromonas gingivalis bacteria are found in dental plaques and have the ability to form biofilms. Biofilms are collections of bacterial cells, which are tightly attached to a surface covered by an extracellular polymer matrix released by the bacteria. Periodontitis therapy generally uses tetracycline antibiotics which if used long-term can cause resistance. Chitosan Rajungan crab extract (Portunus pelagicus) has antimicrobial potential which can be used as an alternative therapy. Objective: To determine the antibacterial power of Rajungan crab chitosan extract (Portunus pelagicus) in various concentrations of Porphyromonas gingivalis bacterial biofilms. Method: This research was true experimental laboratorist. This study used Rajungan crab chitosan extract (Portunus pelagicus) to Porphyromonas gingivalis biofilm tested by the test method biofilm. Divided into 5 groups, where each group consisted of 4 samples. The control group consisted of: K- (negative control group), K + (positive control group / tetracycline group), and 3 treatment groups, namely P1 (0.25% Rajungan crab chitosan), P2 (0.5% Rajungan crab chitosan) , P3 (1% Rajungan crab chitosan). Antibacterial power was determined by the value of Optical density in the ELISA Reader. Data analysis using One Way ANOVA followed by LSD test. Result: There were significant differences (p <0.05) seen from the percentage of biofilm inhibition in the negative control group for all groups, the positive control group for the crab crab chitosan extract concentrations of 0.25% and 0.5%. Conclusion: The chitosan crab chitosan extract (Portunus pelagicus) with 1% concentration has the greatest antibacterial effect on the Porphyromonas gingivalis biofilm
DENTA, Volume 12; doi:10.30649/denta.v12i2.164
Background: Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. Purpose: To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. Methods: This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. Results: The data were analyzed with one way Anova test and showed significant difference among groups (p<0,05). To show the difference between each groups we used LSD test and showed significant difference (p<0,05) between K+ compared with P1, and K+ compared with K-. Conclusion: Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models. Keywords: Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes Correspondence: Agni Febrina Pargaputri, Department of Oral Biology Faculty of Dentistry, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. Phone: 031-5945864, fax: 031-5912191, Email: [email protected]
DENTA, Volume 12; doi:10.30649/denta.v12i2.159
Background: Tooth transposition is a rare and severe positional anomaly that may create many orthodontic problems from both esthetic and functional points of view. In this case report, the orthodontic management of distoversion upper central incisor and transposition of the upper right canine and lateral incisor. The patient was treated with a fixed appliance for 4 months. At this moment the central incisor has already corrected, lateral incisor has also erupted and aligned. Patient management should be continued with phase two treatment to correct the transposition of canine.Keywords: Distoversion, tooth transposition, orthodontic management.Correspondence : Departement of Pediatric Dentistry, College of Dentistry, University of Airlangga, Surabaya, East Java, Indonesia.
DENTA, Volume 12; doi:10.30649/denta.v12i2.176
Background : The denture base made of acrylic resin has a porosity and water absorbtion characteristic to cause the deposition of food waste, that problem was known to cause a growth of Candida albicans microorganisms. This problem can be solved by providing a coating (Surface coating) made of natural polymer (cellulose acrtate) of sea sea grass (Enhalus acoroides) mixed with hydrocoloid material so as to provide protection properties. Such protective properties can inhibit the bonding between the residual monomer results of acrylic resins and microorganisms. Objective : To analyze and compare the number of Candida albicans colonies on the acrylic resin plate that has surface coating and no surface coating. Method : Using post test only group design, using 12 samples divided into 2 groups. Group 1 is the control group (Heat cured acrylic plate contaminated with Candida albicans). Group 2 : Treatment group (Heat cured acrylic plate with surface coating made from sea grass Enhalus acoroides then contaminated with Cndida albicans). Calculating the number of colonies from Candida albicans was done by calculating the turbidity of SDB media (Saboraud Dextrose Broth) using UV-VIS Specrofotometer with 560 λ wave. Data analysis using an Independent T-Test. Results : Acrylic resin plates with surface coating showed significant differences in the number of Candida albicans colonies. The average results in the control group obtained an average number of colonies of 1,58 CFU/ml, while in the treatment group as much as 1,42 CFU/ml. Conclusion : The results show that surface coating can decrease the amount of Candida albicans colonies because it can close the microporosity, prevent a water absorption, and prevent the formation of the pellicle.
DENTA, Volume 11; doi:10.30649/denta.v11i2.97
Background: Heat cure acrylic and nylon thermoplastic valplast and lucitone-FRS brands are the three materials most often used as denture bases. Candida albicans is the dominant microorganism that can cause denture stomatitis. Cleaning denture can be done in two ways, namely the mechanical way with a toothbrush or an ultrasonic cleanser and a chemical method by immersing the denture into the cleaning solution. Chitosan solution is antibacterial and anti fungal which can inhibit the growth of Candida albicans colonies on heat cured acrylic plates, valplast and lucitone-FRS. Purpose: To determine the concentration of chitosan solution and the type of denture plate that most effectively inhibited the growth of Streptococcus mutans Candida albicans colonies on heat cured acrylic, valplast and lucitone-FRS plates. Materials and Methods: 21 heat cured acrylic plate samples, 21 valplast plate samples, 21 lucitone-FRS plate samples measuring 10x10x2 mm divided by 9 groups. Heat cured, valplast and lucitone-FRS acrylic plate samples were contaminated with Candida albicans then immersed using the concentration of 0.25%, 0.5% chitosan solution and sterile aquades as the control group with 90 minutes immersion time. The entire study sample was calculated using Candida albicans colonies on Sabourroud's Dextrose Agar media. Results: The Kruskall Wallis test showed a significant difference (p <0.05) in all treatment groups. Mann-Whitney showed a significant difference (p <0.05) in all groups in the Candida albicans study. Conclusion: Soaking the lucitone-FRS plate in 0.5% chitosan solution for 90 minutes was the most effective in inhibiting the growth of Candida albicans colonies. Keywords:Kitosan, Candida albicans, akrilik heat cured, valplast, lucitone-FRS Correspondence: Anindita Apsari, Department of Prosthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Email : [email protected]/[email protected]
DENTA, Volume 11; doi:10.30649/denta.v11i2.92
Background: Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba hasbeen proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. Purpose: The aim of this study is to determine the cell viability offibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. Materials and Methods: The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. Results: Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). Conclusion: In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells. Keywords: Dental caries, Sonneratia alba, fibroblast, viability, cell cultureCorrespondence: Aprilia, Department of Conservation, Faculty of Dentistry, Hang Tuah Univesity, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]
DENTA, Volume 11; doi:10.30649/denta.v11i2.93
Background: Periodontitis is the most severe complication in oral cavity to diabetes mellitus. Stichopus hermanii contain GAG, a polysaccharide which very useful in wound healing. Hyperbaric oxygen therapy is effective repairing ischemia, hypoxia, and is used as adjuvant therapy. Purpose: To determine effect of administration of Stichopus hermanii (SH) 3% gel and hyperbaric oxygen therapy 3x30 minutes with 5 minute intervals in 7 consecutive days to the number of fibroblasts in ligament periodontal diabetes mellitus rats with periodontitis. Materials and Methods: The experiment design is factorial design. Rats are inducted with STZ single dose 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis. 20 male wistar rats are divided into 5 groups. K- (control), K1 (STZ+P.Gingivalis), K2(STZ+P.Gingivalis+SH), K3(STZ+P.Gingivalis+HBO), K4(STZ+P.Gingivalis+SH+HBO). After 52 days treatment, rats is terminated to make a HPA Sample from the mandible and fibroblast is counted with 400x magnification. Result: One-way Annova here were significant differences (p<0,05) in K- (32.300 ± 11.381), K1 (31.725 ± 5.590), K2 (54.225 ± 7.165), K3 (49.725 ± 10.088), K4 (72.075 ± 9.342). Conclusion: administration of Stichopus hermanii 3% gel combination and 2.4 ATA hyperbaric oxygen 3x30 minutes a day with 5 minutes intervals in 7 consecutive days can increase the number of fibroblasts in diabetes mellitus rats with periodontitis. Keywords: Stichopus hermanii, hyperbaric oxygen, diabetes, periodontitis, fibroblasts Correspondence: Dian Mulawarmanti, Department of Oral Biology,Faculty of Dentistry, , Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]