DENTA, Volume 12; doi:10.30649/denta.v12i2.170
Abstract:Background: Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. Purpose : To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. Material and Method : The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). Results : There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). Conclusion : The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.
DENTA, Volume 12; doi:10.30649/denta.v12i2.175
Abstract:Backgrounds : Enterococcus faecalis is a facultative anaerobic gram-positive bacteria that known to have a role in the infection of the root canal, causing the failure of root canal treatment. The extract from anchovy (Stolephorus insularis) contains fluor has antibacterial properties that can inhibit the growth of gram-positive bacteria and has the potential to be developed as a root canal sterilization agent. Purpose : To determine the antibacterial effect of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. Methods : This study was an true experimental study with post test only control group design and were tested by diffusion methods, consisted of 5 groups: 2 control groups DMSO 1% as negative control and ChKM as positive control, 3 treatment groups of anchovy (Stolephorus insularis) extract with different concentrations of 18%, 24% and 30% which each group consisted of 5 samples. Antivbacterial effect was examined by measuring the clear zone around the filter paper. Data were analyzed by ANOVA test followed by LSD test. Results : Results of this study proved the presence of antibacterial effects of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. In this study, the higher concentrations used, the greater the antibacterial effect. Average inhibition zone of each groups: DMSO 1% (6,03 mm), ChKM (14,18 mm), 18%(6,83 mm), 24%(7,03 mm) and 30%(7,16 mm). Conclusion : Anchovy (Stolephorus insularis) extract has antibacterial effect to the Enterococcus faecalis.
DENTA, Volume 12; doi:10.30649/denta.v12i2.161
Abstract:Background: Candidiasis is one of oral infectious disease caused by Candida albicans.The role of C. albicans as pathogen opportunistic in oral infection can affected by immune system. Phagocytosis has contributed in immune system against C. albicans infection, which is the role of monocytes. Grapthophyllum L. Griff has been proved to increase macrophage’s phagocytic activity, but the effect of violet leaves ethanol extract (EEDU) to increase monocyte’s phagocytic activity on C. albicans is unknown. Objectives: To determine the effect of violet leaves extract on monocyte’s phagocytic activity on C. albicans. Methods: This research was acted in vitro on human monocyte cell culture. There was 5 groups: negative control group, positive control group (incubated in Isoprinosine) and treatment groups was incubated in EEDU 2,5%, 5%, 10%. The percentage of activated monocytes was counted. Result: The result showed that incubation of EEDU 2,5%, 5% and 10% could increase monocyte’s phagocytic activity significantly (p<0,05) compared to control groups. The number of monocyte’s phagocytosis on C. albicans incubated with EEDU 2,5%, 5% and 10% is 65%, 60,5%, and 58,75%. The optimal concentration of EEDU to increase monocyte’s phagocytic activity was 2,5%. Conclusion: The study showed that EEDU can increase monocyte’s phagocytic activity on C. albicans. Keywords:Ethanol extract of Graptophyllum pictum L. Griff leaves, Monocyte’s Phagocytic Activity, Candida albicans
DENTA, Volume 12; doi:10.30649/denta.v12i2.174
Abstract:Background: Porphyromonas gingivalis bacteria are found in dental plaques and have the ability to form biofilms. Biofilms are collections of bacterial cells, which are tightly attached to a surface covered by an extracellular polymer matrix released by the bacteria. Periodontitis therapy generally uses tetracycline antibiotics which if used long-term can cause resistance. Chitosan Rajungan crab extract (Portunus pelagicus) has antimicrobial potential which can be used as an alternative therapy. Objective: To determine the antibacterial power of Rajungan crab chitosan extract (Portunus pelagicus) in various concentrations of Porphyromonas gingivalis bacterial biofilms. Method: This research was true experimental laboratorist. This study used Rajungan crab chitosan extract (Portunus pelagicus) to Porphyromonas gingivalis biofilm tested by the test method biofilm. Divided into 5 groups, where each group consisted of 4 samples. The control group consisted of: K- (negative control group), K + (positive control group / tetracycline group), and 3 treatment groups, namely P1 (0.25% Rajungan crab chitosan), P2 (0.5% Rajungan crab chitosan) , P3 (1% Rajungan crab chitosan). Antibacterial power was determined by the value of Optical density in the ELISA Reader. Data analysis using One Way ANOVA followed by LSD test. Result: There were significant differences (p <0.05) seen from the percentage of biofilm inhibition in the negative control group for all groups, the positive control group for the crab crab chitosan extract concentrations of 0.25% and 0.5%. Conclusion: The chitosan crab chitosan extract (Portunus pelagicus) with 1% concentration has the greatest antibacterial effect on the Porphyromonas gingivalis biofilm
DENTA, Volume 12; doi:10.30649/denta.v12i2.164
Abstract:Background: Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. Purpose: To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. Methods: This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. Results: The data were analyzed with one way Anova test and showed significant difference among groups (p<0,05). To show the difference between each groups we used LSD test and showed significant difference (p<0,05) between K+ compared with P1, and K+ compared with K-. Conclusion: Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models. Keywords: Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes Correspondence: Agni Febrina Pargaputri, Department of Oral Biology Faculty of Dentistry, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. Phone: 031-5945864, fax: 031-5912191, Email: [email protected]
DENTA, Volume 12; doi:10.30649/denta.v12i2.159
Abstract:Background: Tooth transposition is a rare and severe positional anomaly that may create many orthodontic problems from both esthetic and functional points of view. In this case report, the orthodontic management of distoversion upper central incisor and transposition of the upper right canine and lateral incisor. The patient was treated with a fixed appliance for 4 months. At this moment the central incisor has already corrected, lateral incisor has also erupted and aligned. Patient management should be continued with phase two treatment to correct the transposition of canine.Keywords: Distoversion, tooth transposition, orthodontic management.Correspondence : Departement of Pediatric Dentistry, College of Dentistry, University of Airlangga, Surabaya, East Java, Indonesia.
DENTA, Volume 12; doi:10.30649/denta.v12i2.176
Abstract:Background : The denture base made of acrylic resin has a porosity and water absorbtion characteristic to cause the deposition of food waste, that problem was known to cause a growth of Candida albicans microorganisms. This problem can be solved by providing a coating (Surface coating) made of natural polymer (cellulose acrtate) of sea sea grass (Enhalus acoroides) mixed with hydrocoloid material so as to provide protection properties. Such protective properties can inhibit the bonding between the residual monomer results of acrylic resins and microorganisms. Objective : To analyze and compare the number of Candida albicans colonies on the acrylic resin plate that has surface coating and no surface coating. Method : Using post test only group design, using 12 samples divided into 2 groups. Group 1 is the control group (Heat cured acrylic plate contaminated with Candida albicans). Group 2 : Treatment group (Heat cured acrylic plate with surface coating made from sea grass Enhalus acoroides then contaminated with Cndida albicans). Calculating the number of colonies from Candida albicans was done by calculating the turbidity of SDB media (Saboraud Dextrose Broth) using UV-VIS Specrofotometer with 560 λ wave. Data analysis using an Independent T-Test. Results : Acrylic resin plates with surface coating showed significant differences in the number of Candida albicans colonies. The average results in the control group obtained an average number of colonies of 1,58 CFU/ml, while in the treatment group as much as 1,42 CFU/ml. Conclusion : The results show that surface coating can decrease the amount of Candida albicans colonies because it can close the microporosity, prevent a water absorption, and prevent the formation of the pellicle.
DENTA, Volume 11; doi:10.30649/denta.v11i2.95
Abstract:Background:Maxillary expansion uses mechanical forces to open the maxillary suture which causes inflammation and bone remodeling around the area. Stichopus hermanni is known to contain flavonoid, chondroitin sulfate, and saponin which is used to inhibit inflammatory process, increase bone metabolism and mineralization, and wound healing. Purpose: The aim of this experiment is to know the effect of Stichopus hermanni to bigonial width on maxillary suture remodeling expansion using cephalometric analysis. Materials and Methods: Experimental units used were 24 male Cavia cobaya divided into 4 groups (n=6). Group K(-) is negative control. Helical spring was applied and activated in the other 3 groups for 10 days. Group K(+) was given 2% NaCMC gel, both group P1 and P2 was given 3% Stichopus hermanni gel. Group P2 were also given retention period for 10 days after activation period. Cavia cobaya were then decapitated and observed for the bigonial width changes. The data was analyzed with One Way ANOVA test continued with LSD test. Results: The result of ANOVA test showed siginficant differences among the treatment groups p=0,000 (p<0,05). LSD test showed significant differences between group K(-) and K(+) (Sig. 0,005), K(-) and P1 (Sig. 0,001), and group K(-) and P2 (Sig. 0,000). Conclusion: 3% Stichopus hermanni gel increases bigonial width but not significant in remodeling process on maxillary suture expansion. Keywords: Stichopus hermanni, bone remodeling, maxillary suture expansion, bigonial width, cephalometric analysis Correspondence: Noengki Prameswari, Department of Orthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, 5912191, Email: [email protected]
DENTA, Volume 11; doi:10.30649/denta.v11i2.103
Abstract:Background: Nursing bottle carries is a problematic condition in children. On one side, parents try to supply enough nutrition to their children, though on the other side this night-time bottle feeding habit might cause negative effect on the childrens dental health. Esthetic dental treatment on children have improved greatly over the past few years, as parents expect an almost-complete restoration of their child’s dental condition. The alternatives for pediatrics esthetic dental restoration may depend on the cooperativeness and habit of the child themselves, while also taking the child’s development into consideration. Aim: to report evaluation of aesthetic restoration on primary teeth. Case: A 4 year-old female child was presented by her parents, with damaged four anterior teeth, which made her uncomfortable especially on feeding due to the sharp edges. From history taking we found a night-time bottle-feeding habit, which usually occurred until patient was asleep. Case Management: restoration of the four primary incisive teeth was performed using a resin composite crown. Outcome of the treatment was evaluated until the primary teeth exfoliateand permanent teeth arise. Conclusion: cooperation between patient, parents, and dentist is important in the dental restorative treatment on paediatric patient. Moreover, a good communication with parents is encouraged, especially on follow-up schedule, treatment cost, success rate and possibility of treatment failure is also encouraged. Staged evaluation is needed due to the natural substitution of the primary teeth by permanent teeth, besides the development process of the jaws.Keywords: Nursing bottle caries, esthetic restoration, growthanddevelopmentCorrespondence: Annisa Listya Paramita, drg., Sp.KGA. Departemen Ilmu Kedokteran Gigi Anak. Fakultas Kedokteran Gigi Universitas Hang Tuah, Surabaya. Jl. Arif Rahman Hakim No. 150, Surabaya.
DENTA, Volume 11; doi:10.30649/denta.v11i2.94
Abstract:Background: Diabetes mellitus (DM) is a metabolic disorders characterized by hyperglycemia caused by oxidative stress. Porphyromonas gingivalis (P.g) is a periodontopathogen caused periodontitis exacerbate blood glucose levels (BGL). Hyperbaric oxygen therapy (HBOT) combined with stichopus hermanii (SH) has a role as an antioxidant and antibacterial agent. Purpopse: To analysis effect of HBOT, SH powder and combination of both to the level of blood glucose diabetic Wistar rat induced P.g. Materials and Methods: This study was pre-test-post-test control group design, using 20 male Wistar rat were divided into 5 groups, Group1 (STZ), Group2 (STZ+P.g), Group3 (STZ+P.g+HBOT), Group4 (STZ+P.g+SH powder), Group5 (STZ+P.g+HBO+SH). STZ induction intravenous for 4 days in a row. P.g. induction was exposure 3 times for 4 days. After therapy (7 days), sample blood tail vein of rat were taken for measure BGL by glucometer. Result: Level of blood glucose K1(280.25±88.786)mg/dl, K2(372.25±135.662)mg/dl, K3(249.50±96.652)mg/dl, K4(172.00±82.077)mg/dl, K5(104.75±38.257)mg/dl. Conclusion: Induction of STZ increased the BGL but induction of P.g. cannot exacerbate BGL . HBOT combined with SH powder can reduce BGL in STZ and P.g. induction group.Keywords: Diabetes mellitus, periodontitis, blood glucose levels, Stichopus hermanii, hyperbaric oxygen.Correspondence: Yoifah Rizka, Department of Periodontology, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, 5912191, Email address: [email protected]