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(searched for: doi:10.1016/j.snb.2015.08.117)
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Sameer Sonkusale, Maryam Shojaei Baghini, Shuchin Aeron
Published: 15 June 2022
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Nikita Sarawagi, Kalyan Vaid, Jasmeen Dhiman, Treesa Johns,
Published: 26 March 2022
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Mandana Amiri, Simin Arshi, Reyhaneh S. Saberi
Published: 8 December 2021
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Ludger Figura
Published: 19 November 2021
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Ankur Srivastava, Gargi Mishra, ,
Published: 9 November 2021
Materials Letters, Volume 308; https://doi.org/10.1016/j.matlet.2021.131231

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Shipra Verma,
Chemical Engineering and Processing - Process Intensification; https://doi.org/10.1016/j.cep.2021.108708

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Published: 17 July 2021
by MDPI
Journal: Toxins
Abstract:
Mycotoxin contamination of cereals used for feed can cause intoxication, especially in farm animals; therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current trends in food/feed analysis are focusing on the application of biosensor technologies that offer fast and highly selective and sensitive detection with minimal sample treatment and reagents required. The article presents an overview of the recent progress of the development of biosensors for deoxynivalenol and zearalenone determination in cereals and feed. Novel biosensitive materials and highly sensitive detection methods applied for the sensors and the application of these sensors to food/feed products, the limit, and the time of detection are discussed.
Amit Lochab, Megha Saxena, Kajal Jindal, , Vinay Gupta, Reena Saxena
Published: 23 November 2020
Materials Chemistry and Physics, Volume 259; https://doi.org/10.1016/j.matchemphys.2020.124068

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Avinash Kumar Singh, Tarun Kumar Dhiman, Lakshmi G.B. V.S.,
Published: 13 October 2020
Bioelectrochemistry, Volume 137; https://doi.org/10.1016/j.bioelechem.2020.107684

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Shipra Verma,
Published: 9 July 2020
Microfluidics and Nanofluidics, Volume 24, pp 1-15; https://doi.org/10.1007/s10404-020-02364-0

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Ling Fang, Xiaofang Liao, Boyu Jia, Linchun Shi, Linzhi Kang, ,
Published: 30 April 2020
Biosensors and Bioelectronics, Volume 164; https://doi.org/10.1016/j.bios.2020.112255

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Published: 20 April 2020
by MDPI
Journal: Foods
Abstract:
Mycotoxins are the most widely studied biological toxins, which contaminate foods at very low concentrations. This review describes the emerging extraction techniques and the current and alternatives analytical techniques and methods that have been used to successfully detect and identify important mycotoxins. Some of them have proven to be particularly effective in not only the detection of mycotoxins, but also in detecting mycotoxin-producing fungi. Chromatographic techniques such as high-performance liquid chromatography coupled with various detectors like fluorescence, diode array, UV, liquid chromatography coupled with mass spectrometry, and liquid chromatography-tandem mass spectrometry, have been powerful tools for analyzing and detecting major mycotoxins. Recent progress of the development of rapid immunoaffinity-based detection techniques such as immunoassays and biosensors, as well as emerging technologies like proteomic and genomic methods, molecular techniques, electronic nose, aggregation-induced emission dye, quantitative NMR and hyperspectral imaging for the detection of mycotoxins in foods, have also been presented.
Z. Mansurov, Zhazira Supiyeva, , A. Taurbekov, , G. Smagulova, M. Mansurova, M. Biisenbayev, Vladimir Pavlenko
Eurasian Chemico-Technological Journal, Volume 21; https://doi.org/10.18321/ectj885

Abstract:
The adsorption behavior of Au3+ ions on metal electrodes has been studied using an electrochemical quartz crystal microbalance combined with the cyclic voltammetry technique. The experiments were carried out for HAuCl4 using 0.1 mol·L-1 HCl (pH~1) as a background electrolyte solution. The kinetics of electroreduction of Au3+ ions on the rice husk based activated carbon and gold electrodes in chloride electrolytes by the cyclic voltammetry and the electrochemical quartz crystal microbalance with a variation of the scan rate in the range of 5‒50 mV·s-1 has been studied. The diffusion coefficient of Au3+ ions for the tested solution on gold and carbon electrodes was determined by the cyclic voltammetry method on the basis of the Randles-Ševčik equation. It is found that electroreduction of gold goes via the discharge of AuCl4- complexes to the formation of metallic gold with a current efficiency of 97‒99%. The scanning electron microscopic images of the gold adsorbed carbon surface was taken to see gold particles and their morphology. In SEM images, it is clearly seen that the surface of carbon has a relief structure and gold has grown in the form of clusters. The smallest gold nanoparticles that could be examined were 100‒250 nm in diameter on the surface of the c arbon electrode.
, Li-Ye Niu, Jian-Ying Jing, Wan-Ming Zhao
Published: 29 November 2019
Optics & Laser Technology, Volume 124; https://doi.org/10.1016/j.optlastec.2019.105899

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Ping Wang, Fubin Pei, Enhui Ma, Qingshan Yang, Haoxuan Yu, Jiao Liu, Yueyun Li, Qing Liu, Yunhui Dong,
Published: 29 August 2019
Bioelectrochemistry, Volume 131; https://doi.org/10.1016/j.bioelechem.2019.107352

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Zeineb Ben Abdallah, Christine Grauby-Heywang, , Sebastien Cassagnere, Fabien Moroté, Eddie Maillard, Halim Sghaier,
Published: 15 June 2019
Biochemical Engineering Journal, Volume 150; https://doi.org/10.1016/j.bej.2019.107262

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Hongsu Wang, Yi Xiu, Yan Chen, Liping Sun, Libin Yang, Honghao Chen, Xiaodi Niu
Published: 24 May 2019
Journal: RSC Advances
RSC Advances, Volume 9, pp 16278-16287; https://doi.org/10.1039/c9ra00907h

Abstract:
The outbreak of food-borne pathogens has become a serious concern; therefore, the detection of pathogenic bacteria in food is required. Untreated, sensitive, and reliable sensors should be developed for the detection of Staphylococcus aureus (S. aureus). In this study, a sensitive antibody-based electrochemical immunosensor was developed using antibody (Ab)-hierarchical mesoporous silica (HMS) bio-conjugates for label-free detection of low concentrations of S. aureus. First, a bio-template method based on butterfly wings was used to prepare the HMS. Then, the carrier material was amino-functionalized to cross-link the antibody with glutaraldehyde. The Ab-HMS bio-conjugates were then immobilized on a glassy carbon electrode (GCE), and the presence of S. aureus was detected by analyzing the changes in the peak currents after the antigen–antibody complex formation. Differential pulse voltammetry (DPV) was performed with bacterial concentrations ranging from 10 to 2 × 103 colony forming units (CFU) mL−1. Selective tests were performed using Escherichia coli (E. coli), Listeria monocytogenes (L. monocytohenes), and Salmonella, and the selective assays showed specific detection of S. aureus using the sensor. In addition, the immunosensor showed a good linear relationship between the peak current increase and logarithmic S. aureus concentration (R2 = 0.9759) with a fast detection time (20 min) and detection limit of 11 CFU mL−1. When the electrochemical impedance spectroscopy (EIS) was performed under the same conditions, the results showed a good linear relationship between the impedance change value and the bacterial concentration (R2 = 0.9720), the limit of detection (LOD) was 12 CFU mL−1. The performance of the sensor was compared with that of the colony counting method in the spiked milk sample test. The results showed no significant difference in the test results. Hence, this electrochemical immunosensor can be used to quickly detect S. aureus in actual food samples with a high sensitivity, specificity and stability.
Philana Nolan, Sanna Auer, Anna Spehar, Christopher T. Elliott,
Published: 3 April 2019
Food Additives & Contaminants: Part A, Volume 36, pp 800-814; https://doi.org/10.1080/19440049.2019.1595171

Abstract:
There are an ample number of commercial testing kits available for mycotoxin analysis on the market today, including enzyme-linked immunosorbent assays, membrane-based immunoassays, fluorescence polarisation immunoassays and fluorometric assays. It can be observed from the literature that not only are developments and improvements ongoing for these assays but there are also novel assays being developed using biosensor technology. This review focuses on both the currently available methods and recent innovative methods for mycotoxin testing. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid on-site analysis, indicating the possible detection methods that will shape the future market.
Published: 4 March 2019
by MDPI
Journal: Chemosensors
Abstract:
Modern analysis of food and feed is mostly focused on development of fast and reliable portable devices intended for field applications. In this review, electrochemical biosensors based on immunological reactions and aptamers are considered in the determination of mycotoxins as one of most common contaminants able to negatively affect human health. The characteristics of biosensors are considered from the point of view of general principles of bioreceptor implementation and signal transduction providing sub-nanomolar detection limits of mycotoxins. Moreover, the modern trends of bioreceptor selection and modification are discussed as well as future trends of biosensor development for mycotoxin determination are considered.
Published: 1 December 2018
Journal: Food chemistry
Food chemistry, Volume 268, pp 342-346; https://doi.org/10.1016/j.foodchem.2018.06.101

Abstract:
As one of the most toxic mycotoxins, aflatoxin B1 (AFB1) is a major food pollutant which can pose a high risk to human health. In this work, an accurate fluorescent sensing method was proposed for AFB1 determination, based on hairpin structure of G-quadruplex oligonucleotide-Aptamer chimera, silica nanoparticles coated with streptavidin (SNPs-Streptavidin) and N-methyl mesoporphyrin IX (NMM). The hairpin structure of chimera and SNPs-Streptavidin allowed AFB1 detection with high sensitivity and specificity. Moreover, the developed sensor could detect AFB1 in 30 min. The relative fluorescence intensity increased as AFB1 concentrations increased with a linear range of 30-900 pg/mL and a limit of detection (LOD) of 8 pg/mL. The constructed aptasensor was successfully employed to assess AFB1 spiked grape juice and human serum samples. The analytical recovery of AFB1 in the grape juice samples ranged from 95-106%, implying the great potential of the presented aptasensor in food product analysis.
Published: 1 November 2018
by MDPI
Journal: Biosensors
Biosensors, Volume 8; https://doi.org/10.3390/bios8040104

Abstract:
In biomedical science among several other growing fields, the detection of specific biological agents or biomolecular markers, from biological samples is crucial for early diagnosis and decision-making in terms of appropriate treatment, influencing survival rates. In this regard, immunosensors are based on specific antibody-antigen interactions, forming a stable immune complex. The antigen-specific detection antibodies (i.e., biomolecular recognition element) are generally immobilized on the nanomaterial surfaces and their interaction with the biomolecular markers or antigens produces a physico-chemical response that modulates the signal readout. Lowering the detection limits for particular biomolecules is one of the key parameters when designing immunosensors. Thus, their design by combining the specificity and versatility of antibodies with the intrinsic properties of nanomaterials offers a plethora of opportunities for clinical diagnosis. In this review, we show a comprehensive set of recent developments in the field of nanoimmunosensors and how they are progressing the detection and validation for a wide range of different biomarkers in multiple diseases and what are some drawbacks and considerations of the uses of such devices and their expansion.
Ying Tang, , Jin Zhang,
Published: 1 November 2018
Analytica chimica acta, Volume 1031, pp 161-168; https://doi.org/10.1016/j.aca.2018.05.027

Abstract:
A simple and sensitive quartz crystal microbalance (QCM) immunosensing platform was designed for the high-efficient detection of aflatoxin B1 (AFB1) in foodstuff. Initially, phenoxy-derived dextran molecule was immobilized on the surface of QCM gold substrate by using thiolated β-cyclodextrin based on the supramolecular host-guest chemistry between phenoxy group and cyclodextrin. Then, AFB1-bovine serum albumin (AFB1-BSA)-conjugated concanavalin A (Con A) was assembled onto the QCM probe through the dextran-Con A interaction. Glucose-loaded nanoliposome, labeled with monocolonal anti-AFB1 antibody, was used for the amplification of QCM signal. Upon target AFB1 introduction, the analyte competed with the immobilized AFB1-BSA on the probe for the labeled anti-AFB1 antibody on the nanoliposome. Based on specific antigen-antibody reaction, the amount of the conjugated nanoliposomes on the QCM probe gradually decreased with the increment of target AFB1 in the sample. Upon injection of Triton X-100 in the detection cell, the carried nanoliposome was lysed to release the encapsulated glucose molecules. Thanks to the stronger affinity of Con A toward glucose than that of dextran, AFB1-BSA-labeled Con A was displaced from the QCM probe, resulting in the change of the local frequency. Under the optimum conditions, the shift of the functionalized QCM immunosensing interface in the frequency shift was proportional to the concentration of target AFB1 within a dynamic range from 1.0 ng kg−1 to 10 μg kg-1 at a low detection limit of 0.83 ng kg−1. In addition, the acceptable assayed results on precision, reproducibility, specificity and method accuracy for the analysis of real samples were also acquired. Importantly, our strategy can provide a signal-on competitive immunoassay for the detection of small molecules, e.g., mycotoxins and biotoxins, thereby representing a versatile sensing schemes by controlling the corresponding antibody or hapten in the analysis of food safety.
Fuyuan Zhang, Bing Liu, Yan Zhang, Junping Wang, Yang Lu, Juan Deng,
Published: 26 September 2018
Analytica chimica acta, Volume 1047, pp 139-149; https://doi.org/10.1016/j.aca.2018.09.058

Abstract:
In order to develop a sensitive immunoassay for Aflatoxin B1 (AFB1) monitoring, a hybridoma secreting anti-AFB1 monoclonal antibody with high binding affinity was screened. A new type of CdTe/CdS/ZnS quantum dot was synthesized and conjugated with an artificial antigen for use as a fluorescent probe in a simple one-step fluorescence immunoassay (FLISA). The developed FLISA exhibited a sensitive determination of AFB1 in cereal samples in a wide linear range, from 0.08 to 1.97 ng mL-1, with a detection limit of 0.01 ng mL-1 in cereal samples. The corresponding immunoglobulin genes of the Fab fragment were cloned and sequenced, and expression of Fab was successfully verified in HEK293 cells, with a Kd value of 1.09×10-7 mol L-1 to AFB1. To investigate the interactions between the antibody and AFB1, molecular docking, molecular dynamic simulation, and quantum-chemical computation were performed on AFB1 and a homology model of the Fab fragment. Our results showed that residues Ser32, Trp93, and Trp98 played the most important roles in the binding through hydrogen bond formation, Pi-alkyl interaction, and van der Waals interaction. In addition, the electrostatic potential study of AFB1 demonstrated that electrostatic interaction also played an important role in the recognition process. The theory study results provided guidance for hapten design and antibody improvement through genetic engineering.
Bo-Tao Wang,
IEEE Sensors Journal, Volume 18, pp 8303-8310; https://doi.org/10.1109/jsen.2018.2866169

Abstract:
An optical fiber biosensor based on gold nanoparticles and protein A co-modified Au film coated photonic crystal fiber (Au-PCF) is proposed and demonstrated for human IgG detection. For the electric field coupling effect between surface plasmon resonance (SPR) of Au film and localized surface plasmon resonance (LSPR) of gold nanoparticles, the refractive index sensitivity can be enhanced significantly. Besides, goat anti-human IgG is immobilized by protein A modified on the gold nanoparticles surface to interact with human IgG. Protein A can specifically bind the Fc region of the antibodies and has a high degree of orientation for capturing antibodies. Experimental results indicate that the refractive index sensitivity of Au-PCF sensor modified gold nanoparticles reaches 3915 nm/RIU, which is about 1.6 times higher than the Au-PCF sensor without gold nanoparticles modified. The lowest detection limit of human IgG based on gold nanoparticles and protein A co-modified Au-PCF sensor is 37 ng/mL, which is about 2.4 times and 6.3 times lower than gold nanoparticles-Au-PCF sensor and Au-PCF sensor, respectively. Such a sensitivity enhanced optical fiber biosensor based on SPR-LSPR coupling effect has potential application in clinical disease diagnosis and immunoassays.
, Hongbo Wang, , Zhenguo Ji
Published: 1 June 2018
Materials Research Express, Volume 5; https://doi.org/10.1088/2053-1591/aaca0d

Abstract:
The detection of aflatoxin B1 (AFB1) using immunoassays, especially electrochemical immunoassays, is fast, sensitive and efficient. In this study, 2-aminoethanethiol was used to enhance the speed and sensitivity of conventional electrochemical immunoassays for AFB1 detection by assembly on the surface of a Au electrode, forming self-assembled monolayers (SAMs). Then, non-competitive immunoassays occurred on the modified electrode surface forming an electrochemical immunoassay sensor. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy were used to examine the state of the SAMs, and an electrochemical workstation was used to monitor the current change of the electrochemical reaction, so as to characterize the designed immunosensor. Our experimental results shows that, the 2-aminoethanethiol reagent was successfully assembled on the Au surface through Au-S bonding and the –NH2 terminal group faced outward. Herein, the minimum concentration of AFB1 which caused a significant current change was 0.01 ng/mL. The prepared immunosensor also exhibited excellent stability and sensitivity after storage for 7 days or upon regeneration.
, , Bettina Takács, István Szendrő, George Szakacs, , Eszter Tóth-Szeles, , Diána Weiser, , et al.
Published: 23 April 2018
Journal: Food chemistry
Food chemistry, Volume 267, pp 10-14; https://doi.org/10.1016/j.foodchem.2018.04.089

Abstract:
Mycotoxins, present in a wide range of food and feed commodities, are toxic secondary metabolites produced by a number of different fungi. Certain mycotoxins do not readily degrade at high temperatures, therefore are resistant to food processing, and consequently are present in the human and animal food supply. Optical waveguide lightmode spectroscopy (OWLS) was applied for the detection of aflatoxin B1, in a competitive immunoassay format, to compare the analytical sensitivity achieved with an immunosensor design allowing signal enhancement by increasing the sensor surface through immobilization of gold nanoparticles (AuNPs) of different size and origin (obtained by chemical or biotechnological synthesis). The effects of AuNPs median size, the methods of sensitization and the biochemical parameters on immunosensor performace were examined. After optimization of the sensitized sensor surface, an immunosensing method was developed for the analysis of aflatoxin in paprika matrix and the results were compared with HPLC reference measurements.
Tamal Sarkar, Neethu Narayanan,
International Journal of Environmental Research, Volume 11, pp 591-601; https://doi.org/10.1007/s41742-017-0052-0

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, Noor Mohammad Danesh, , , Ahmad Sarreshtehdar Emrani, Reza Zolfaghari,
Published: 1 August 2017
Biosensors and Bioelectronics, Volume 94, pp 374-379; https://doi.org/10.1016/j.bios.2017.03.028

Abstract:
There is a prompt need for determination of aflatoxin B (AFB) in food products to avoid distribution and consumption of contaminated food products. In this study, an accurate electrochemical sensing strategy was presented for detection of AFB based on aptamer (Apt)-complementary strands of aptamer (CSs) complex which forms a π-shape structure on the surface of electrode and exonuclease I (Exo I). The presence of π-shape structure as a double-layer physical barrier allowed detection of AFB with high sensitivity. In the absence of AFB, the π-shape structure remained intact, so only a weak peak current was recorded. Upon the addition of AFB, the π-shape structure was disassembled and a strong current was recorded following the addition of Exo I. Under optimal conditions, the electrochemical signals enhanced as AFB concentrations increased with a dynamic range of 7-500pg/mL and a limit of detection (LOD) of 2pg/mL. The developed aptasensor was also used to analyze AFB spiked human serum and grape juice samples and the recoveries were 95.4-108.1%.
Jeffery Alexander Powell, ,
Published: 3 July 2017
Journal: RSC Advances
RSC Advances, Volume 7, pp 33688-33700; https://doi.org/10.1039/c7ra01970j

Abstract:
We report in this study, the development of a polymorphic biosensitive Si nanocore superstructure as a SERS biosensing platform.
, , , Massimo Pica Ciamarra, Carlo Altucci, Rosanna Capparelli, ,
Published: 9 February 2017
Journal: PLoS ONE
Abstract:
Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue in biosensing since the signal they induce in the transducer, and specifically in a Quartz-Crystal Microbalance, is undetectable. A pesticide like parathion (M = 292 Da) is a typical example of contaminant for which a signal amplification procedure is desirable. The ballasting of the analyte by gold nanoparticles has been already applied to heavy target as proteins or bacteria to improve the limit of detection. In this paper, we extend the application of such a method to small analytes by showing that once the working surface of a Quartz-Crystal Microbalance (QCM) has been properly functionalized, a limit of detection lower than 1 ppb is reached for parathion. The effective surface functionalization is achieved by immobilizing antibodies upright oriented on the QCM gold surface by a simple photochemical technique (Photonic Immobilization Technique, PIT) based on the UV irradiation of the antibodies, whereas a simple protocol provided by the manufacturer is applied to functionalize the gold nanoparticles. Thus, in a non-competitive approach, the small analyte is made detectable by weighing it down through a “sandwich protocol” with a second antibody tethered to heavy gold nanoparticles. The immunosensor has been proved to be effective against the parathion while showing no cross reaction when a mixture of compounds very similar to parathion is analyzed. The immunosensor described in this paper can be easily applied to any small molecule for which polyclonal antibodies are available since both the functionalization procedure of the QCM probe surface and gold nanoparticle can be applied to any IgG, thereby making our device of general application in terms of target analyte.
Reza Eivazzadeh-Keihan, Paria Pashazadeh, Maryam Hejazi, Miguel de la Guardia,
Published: 1 February 2017
TrAC Trends in Analytical Chemistry, Volume 87, pp 112-128; https://doi.org/10.1016/j.trac.2016.12.003

Published: 4 March 2016
Biosensors and Bioelectronics, Volume 81, pp 532-545; https://doi.org/10.1016/j.bios.2016.03.004

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