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(searched for: doi:10.4236/ojab.2014.32002)
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Published: 4 February 2022
by MDPI
Journal: Biosensors
Biosensors, Volume 12; https://doi.org/10.3390/bios12020095

Abstract:
Graphene-oxide and ionic liquid composite-modified pencil graphite electrodes (GO-IL-PGEs) were developed and used as a sensing platform for breast cancer 1 (BRCA1) gene detection. The characterization of GO-IL modified electrodes was executed by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The nucleic-acid hybridization was monitored by a differential pulse voltammetry (DPV) technique by directly measuring the guanine oxidation signal without using any indicator. The effects of the IL concentration, the probe concentration, and the hybridization time were optimized to the biosensor response. The limit of detection (LOD) was calculated in the concentration range of 2–10 μg/mL for the BRCA1 gene and found to be 1.48 µg/mL. The sensitivity of the sensor was calculated as 1.49 µA mL/µg cm2. The developed biosensor can effectively discriminate the complementary target sequence in comparison to a three-base-mismatched sequence or the non-complementary one.
Yawen Wu, , Changjun Hou, Peng Lu, Lan Peng, Jiawei Li, , Danqun Huo
Published: 15 November 2021
Journal: The Analyst
The Analyst, Volume 147, pp 862-869; https://doi.org/10.1039/d1an01759d

Abstract:
Human epidermal growth factor receptor 2 (HER2) is one of the specific markers of breast cancer, which is of great significance to the early diagnosis and prognosis of breast cancer.
Yash Amethiya, Prince Pipariya, Shlok Patel,
Published: 22 October 2021
Intelligent Medicine, Volume 2, pp 69-81; https://doi.org/10.1016/j.imed.2021.08.004

The publisher has not yet granted permission to display this abstract.
Muhamad Huzaifah Omar, Khairunisak Abdul Razak, ,
Published: 6 May 2021
Journal: RSC Advances
RSC Advances, Volume 11, pp 16557-16571; https://doi.org/10.1039/d1ra01987b

Abstract:
This minireview discusses the current on-demand applications of the conductive 3D-printed electrodes based upon polymer/carbon nanomaterial filaments, printed using the FDM 3D printing method, in developing electrochemical sensors and biosensors.
Hassan Nasrollahpour, Ibrahim Isildak, Mohammad-Reza Rashidi, Esmat Alsadat Hashemi, Abdolhosein Naseri,
Published: 1 May 2021
Cancer Nanotechnology, Volume 12, pp 1-16; https://doi.org/10.1186/s12645-021-00082-y

Abstract:
Background: In this label-free bioassay, an electrochemiluminescence (ECL) immunosensor was developed for the quantification of breast cancer using HER-2 protein as a metastatic biomarker. Method: For this purpose, the ECL emitter, [Ru(bpy)3]2+, was embedded into biocompatible chitosan (CS) polymer. The prepared bio-composite offered high ECL reading due to the depletion of human epidermal growth factor receptor 2 (HER-2) protein. Reduced graphene oxide (rGO) was used as substrate to increase signal stability and achieve greater sensitivity. For this, rGO was initially placed electrochemically on the glassy carbon electrode (GCE) surface by cyclic voltammetry (CV) technique. Next, the prepared CS/[Ru(bpy)3]2+ biopolymer solution was coated on a drop of the modified electrode such that the amine groups of CS and the carboxylic groups of rGO could covalently interact. Using EDC/NHS chemistry, monoclonal antibodies (Abs) of HER-2 were linked to CS/[Ru(bpy)3]2+/rGO/GCE via amide bonds between the carboxylic groups of Ab molecules and amine groups of CS. The electrochemical behavior of the electrode was studied using different electrochemical techniques such as electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) and also ECL tests. Results: After passing all optimization steps, the lower limit of detection (LLOQ) and linear dynamic range (LDR) of HER-2 protein were practically obtained as 1 fM and 1 fM to 1 nM, individually. Importantly, the within and between laboratory precisions were performed and the suitable relative standard deviations (RSDs) were recorded as 3.1 and 3.5%, respectively. Conclusions: As a proof of concept, the designed immunosensor was desirably applied for the quantification of HER-2 protein in breast cancer suffering patients. As a result, the designed ECL-based immunosensor has the capability of being used as a conventional test method in biomedical laboratories for early detection of HER-2 protein in biological fluids. Graphic
, Nor Azah Yusof, , Suriana Sabri, , Shuhaimi Mustafa, Nurul Asyikeen Ab Mutalib, Shinobu Sato, Shigeori Takenaka, Nor Azizah Parmin, et al.
Published: 22 July 2020
Journal: RSC Advances
RSC Advances, Volume 10, pp 27336-27345; https://doi.org/10.1039/d0ra03585h

Abstract:
An electrochemical DNA biosensor was developed based on the fabrication of silicon nanowires/platinum nanoparticles on a screen-printed carbon electrode for the detection of Sus scrofa mitochondrial DNA in food.
Nuzhat Jamil, Waheed S. Khan
Published: 14 February 2020
The publisher has not yet granted permission to display this abstract.
Vjaceslavs Gerbreders, , Eriks Sledevskis, Andrejs Gerbreders, Irena Mihailova, Edmunds Tamanis,
Published: 27 January 2020
Journal: CrystEngComm
CrystEngComm, Volume 22, pp 1346-1358; https://doi.org/10.1039/c9ce01556f

Abstract:
Optimal synthesis parameters for hydrothermal growth of nine ZnO nanostructure morphologies as a durable, homogeneous coating have been determined.
, Marianna-Thalia Nikolelis, Spyridoula Bratakou, Vasillios N. Psychoyios
Published: 26 January 2020
The publisher has not yet granted permission to display this abstract.
Published: 22 November 2019
by MDPI
Journal: Sensors
Sensors, Volume 19; https://doi.org/10.3390/s19235111

Abstract:
A toxicity electrochemical DNA biosensor has been constructed for the detection of carcinogens using 24 base guanine DNA rich single stranded DNA, and methylene blue (MB) as the electroactive indicator. This amine terminated ssDNA was immobilized onto silica nanospheres and deposited on gold nanoparticle modified carbon-paste screen printed electrodes (SPEs). The modified SPE was initially exposed to a carcinogen, followed by immersion in methylene blue for an optimized duration. The biosensor response was measured using differential pulse voltammetry. The performance of the biosensor was identified on several anti-cancer compounds. The toxicity DNA biosensor demonstrated a linear response range to the cadmium chloride from 0.0005 ppm to 0.01 ppm (R2 = 0.928) with a limit of detection at 0.0004 ppm. The biosensor also exhibited its versatility to screen the carcinogenicity of potential anti-cancer compounds.
Published: 27 April 2019
by MDPI
Journal: Applied Sciences
Applied Sciences, Volume 9; https://doi.org/10.3390/app9091745

Abstract:
The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.
Vjaceslavs Gerbreders, Marina Krasovska, Irena Mihailova, , , Andrejs Gerbreders, Edmunds Tamanis, Inese Kokina, Ilona Plaksenkova
Published: 14 March 2019
Sensing and Bio-Sensing Research, Volume 23; https://doi.org/10.1016/j.sbsr.2019.100276

The publisher has not yet granted permission to display this abstract.
Hosna Ehzari, Meysam Safari,
Published: 29 July 2018
Microchemical Journal, Volume 143, pp 118-126; https://doi.org/10.1016/j.microc.2018.07.033

The publisher has not yet granted permission to display this abstract.
Aqmar N.Z.N, , Z.M. Zain, S. Rani
IOP Conference Series: Materials Science and Engineering, Volume 340; https://doi.org/10.1088/1757-899x/340/1/012016

Abstract:
This paper addresses the development of differential pulse voltammetry (DPV) embedded algorithm using an ARM cortex processor with new developed potentiostat circuit design for in-situ 3-electrode cell sensing. This project is mainly to design a low cost potentiostat for the researchers in laboratories. It is required to develop an embedded algorithm for analytical technique to be used with the designed potentiostat. DPV is one of the most familiar pulse technique method used with 3-electrode cell sensing in chemical studies. Experiment was conducted on 10mM solution of Ferricyanide using the designed potentiostat and the developed DPV algorithm. As a result, the device can generate an excitation signal of DPV from 0.4V to 1.2V and produced a peaked voltammogram with relatively small error compared to the commercial potentiostat; which is only 6.25% difference in peak potential reading. The design of potentiostat device and its DPV algorithm is verified.
, Sonia, Swati Mahendru, Pankaj Tyagi,
Published: 22 November 2017
Integrated Ferroelectrics, Volume 185, pp 134-143; https://doi.org/10.1080/10584587.2017.1370349

Abstract:
Biosensors having the recognition element as DNA/RNA oligon-ucleotides are known as genosensors, which have been widely utilized for many applications including clinical diagnostic tool for diseases like cancer and a range of infectious diseases. This review aims to discuss the vast repertoire of nanomaterial based genosensors, their designing and numerous applications, including their usage in food quality assessment, environmental monitoring, as aptasensors for the recognition of nucleosides, as biomarkers for the identification of DNA methylation in epigenetics and molecular beacon nano-sensors for probing the living cancer cells etc. Genosensors are of utmost significance for solving various puzzles related to cellular processes and their control mechanisms.
Published: 9 November 2017
by MDPI
Journal: Nanomaterials
Nanomaterials, Volume 7; https://doi.org/10.3390/nano7110381

Abstract:
This article provides an overview of the recent development of ZnO nanowires (NWs) for chemoresistive sensing. Working mechanisms of chemoresistive sensors are unified for gas, ultraviolet (UV) and bio sensor types: single nanowire and nanowire junction sensors are described, giving the overview for a simple sensor manufacture by multiple nanowire junctions. ZnO NW surface functionalization is discussed, and how this effects the sensing is explained. Further, novel approaches for sensing, using ZnO NW functionalization with other materials such as metal nanoparticles or heterojunctions, are explained, and limiting factors and possible improvements are discussed. The review concludes with the insights and recommendations for the future improvement of the ZnO NW chemoresistive sensing.
Published: 31 August 2017
by MDPI
Journal: Sensors
Sensors, Volume 17; https://doi.org/10.3390/s17091993

Abstract:
The rapid and accurate determination of specific circulating biomarkers at different molecular levels with non- or minimally invasive methods constitutes a major challenge to improve the breast cancer outcomes and life quality of patients. In this field, electrochemical biosensors have demonstrated to be promising alternatives against more complex conventional strategies to perform fast, accurate and on-site determination of circulating biomarkers at low concentrations in minimally treated body fluids. In this article, after discussing briefly the relevance and current challenges associated with the determination of breast cancer circulating biomarkers, an updated overview of the electrochemical affinity biosensing strategies emerged in the last 5 years for this purpose is provided highlighting the great potentiality of these methodologies. After critically discussing the most interesting features of the electrochemical strategies reported so far for the single or multiplexed determination of such biomarkers with demonstrated applicability in liquid biopsy analysis, existing challenges still to be addressed and future directions in this field will be pointed out.
, Chandan Singh, Saurabh Srivastava, Prasad Admane, Ved V. Agrawal, Gajjala Sumana, , Amulya Panda, Liang Dong,
Published: 19 July 2017
Journal: RSC Advances
RSC Advances, Volume 7, pp 35982-35991; https://doi.org/10.1039/c7ra05491b

Abstract:
We report a universal protocol for the in situ bioinspired green synthesis of metal nanoparticles on simultaneously reduced graphene oxide sheets using a black pepper extract for quantification of breast cancer biomarker.
Xiaojiao Qin, , Li Deng, Rongfu Huang,
Published: 1 November 2016
Biosensors and Bioelectronics, Volume 85, pp 957-963; https://doi.org/10.1016/j.bios.2016.05.076

Abstract:
In this work, we have developed an electrochemical sensor for label-free and ultrasensitive detection of DNA (exemplified by breast cancer 1gene) by using a photocatalytic reaction. Upon recognition of target DNA, the ethidium bromide molecules which were embedded in the hybridized double strand DNA (dsDNA, target DNA and capture DNA) could photo-catalytically generate singlet oxygen upon green light emitting diode irradiation, leading to an efficient cleave of the dsDNA. As a result, the voltammetry for the [Fe(CN)6]3-/4- was improved increased remarkably because of less blocking of electrode and weaker charge repulsion. Such a simple strategy provided an ultrasensitive detection of breast cancer 1 gene down to the attomolar level with a broad linear range (10 aM-100 nM). The sensor has been the most sensitive eletrochemical methods for detection of breast cancer 1 gene by far in comparison with those without an amplification procedure. Also the sensor can discriminate mismatched DNA from perfectly matched target DNA with high selectivity. Therefore, simplicity, high sensitivity and specificity provided by this photocatalytic eletrosensor will make it a promising tool for early diagnosis of gene-related diseases.
Published: 1 April 2016
Materials Science and Engineering: C, Volume 61, pp 506-515; https://doi.org/10.1016/j.msec.2015.12.091

Abstract:
A sensitive electrochemical DNA biosensor was developed for Helicobacter pylori (H. pylori) detection using differential pulse voltammetry. Single-stranded DNA probe was immobilized on a graphene oxide/gold nanoparticles modified glassy carbon electrode (GO/AuNPs/GCE). A hybridization reaction was conducted with the target DNA and the immobilized DNA on the electrode surface. Oracet blue (OB) was selected for the first time as a redox indicator for amplifying the electrochemical signal of DNA. Enhanced sensitivity was achieved through combining the excellent electric conductivity of GO/AuNPs and the electroactivity of the OB. The DNA biosensor displayed excellent performance to demonstrate the differences between the voltammetric signals of the OB obtained from different hybridization samples (non-complementary, mismatch and complementary DNAs). The proposed biosensor has a linear range of 60.0-600.0 pM and a detection limit of 27.0 pM for detection of H. pylori. In addition, the biosensor have responded very well in the simulated real sample evaluations, signifying its potential to be used in future clinical detection of the H. pylori bacteria.
Anu Singh, Meenakshi Choudhary, M.P. Singh, H.N. Verma, ,
Published: 8 April 2015
Bioelectrochemistry, Volume 105, pp 7-15; https://doi.org/10.1016/j.bioelechem.2015.03.005

The publisher has not yet granted permission to display this abstract.
Published: 17 March 2015
Journal: Nanoscale
Nanoscale, Volume 7, pp 7234-7245; https://doi.org/10.1039/c5nr00194c

Abstract:
We report the fabrication of an efficient, label-free, selective and highly reproducible immunosensor with unprecedented sensitivity (femto-molar) to detect a breast cancer biomarker for early diagnostics.
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