(searched for: doi:10.48112/bcs.v1i4.284)
Published: 1 January 2023
Journal: Biomedicine and Chemical Sciences
Biomedicine and Chemical Sciences, Volume 2, pp 58-62; https://doi.org/10.48112/bcs.v2i1.381
Smart phone used as supporting hardware in different applications in chemical analysis are becoming increasingly important in everyday life. Rapid, easy, and straightforward analytical system flow injection analysis system for the determination of the total phenols was conducted using 4-aminoantipyrine reagent. The detection method was based on the reaction total phenols with reagent in the basic media and subsequent formation of a yellow color product. The samples or standard solutions were injection into a carrier stream to react with 4-aminoantipyrine reagent and ammonium chloride with ammonium hydroxide to give yellow color product, which was detected by spectrophotometer at 510 nm. The experimental condition such as flow rate of reagent and carrier, reagent volume, length of reaction coil and concentration of reagent were optimized. A good linear calibration curve in the range of 250-2000 mg L-1 was obtained with regression equation (y=0.0108 x + 0.3453), (R= 0.9989). The limit of detection was in the amount of 0.0112 mg L-1. The method was successfully applied for the determination of the total phenols in wastewater.
Published: 23 December 2022
Al-Kufa University Journal for Biology, Volume 14, pp 76-82; https://doi.org/10.36320/ajb/v14.i3.11182
Ultrasound waves are caused by oscillating molecular motions in a propagation medium. The waves have a very high frequency (20 kHz). Flow injection analysis is can analyze multiple samples, high accuracy, a very fast response time , modern technical devices that are small. This research intended to study the efficiency of ultrasound waves were determined total phenolic compounds in wastewater .Smart phone was used to investigate results and optimum conditions .The sample or standard phenol solution were injected into a carrier stream to react with 4-aminoantipyrine reagent then expose it to ultrasound waves (46 kHz) which was detected by spectrophotometer. A good linear calibration curve in the range of (250-2000 mg L-1 ) was obtained with regression equation ( y=0.0108 x + 0.3453) ,( R= 0.9989 ) . Results of the experiments showed that the ultrasonic method significantly decrease peak height after using ultrasound waves because of low measurement sensitivity due to effected on bonding process between total phenol compounds and the reagent, and the effect on the bonding of the components of the reagent .