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(searched for: doi:10.14499/indonesianjcanchemoprev11iss1pp1-6)
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N D Amalina, , Harjito
Published: 1 June 2021
Journal of Physics: Conference Series, Volume 1918; https://doi.org/10.1088/1742-6596/1918/3/032006

Abstract:
Currently, nanoparticle is at fastest pace due to the enhanced implication in drug delivery system. The synthesis of chitosan nanoparticles through ionic gelation methods is becoming increasingly relevant due to the low cost involved and environment-friendly approach. In this study, cytotoxic effect of chitosan-Citrus aurantium peels extract nanoparticles (NPs-CA) against MDA-MB-231 breast cancer cells was investigated. The initial confirmations of NPs-CA were using Particles size analysis (PSA), which showed the size of NPs-CA. The Zeta potential was measured to check the stability of NPs-CA. The cytotoxic effect was carried out in MDA-MB-231 breast cancer cells under MTT assay. Based on PSA analysis, the size of F1 and F2 were ranging between 372 – 4472 nm and 94 – 373 nm, respectively. The zeta potential mean value of F2 NPs-CA 34,9 mV, it can be concluded that the particles show less chances for agglomeration. On the various test concentration, the F2 NPs-CA performed better cytotoxic effects than Citrus aurantium peels extract with IC50 value of 83 μg/mL and 320 μg/mL, respectively. These findings pose a positive impact to expand further studies in the development of nanoparticle to cancer therapy.
S Mursiti, , A Marianti
Published: 1 June 2021
Journal of Physics: Conference Series, Volume 1918; https://doi.org/10.1088/1742-6596/1918/5/052005

Abstract:
Excessive Reactive oxygen species (ROS) level can lead to the activation of apoptosis and inhibit metastasis through the NF-κB pathway. Therapeutic approaches by increasing ROS level may be effective strategic in cancer treatment. Citrus maxima (CM) is one of a natural medicinal plant that exhibits cytotoxic effect on several cancer cells. However, the CM extract effect to regulate ROS level remains unclear. Therefore, in this study aims to evaluated the cytotoxic effects of CM extract on metastasis breast cancer MDA-MD-231 cells. Cytotoxic activity of CM extract was evaluated using MTT assay, ROS levels was calculated under flowcytometry. Furthermore, the binding interaction between phytoconstituents and protein that responsible on the ROS metabolic pathway was analyzed under in silico molecular docking. The presence of CM extract increased cells death with IC50 value of 338 μg/mL for 24 h through induction of ROS levels. Molecular docking studies revealed that the hesperidin displayed the best binding energies at -21.4766 (NF-κB) kcal/mol. The binding interaction of hesperidin with the active site of NF-κB protein suggested that amino acid residues (His537, Asp519, Gly407, Gln479, Arg416) might play a role in ROS metabolism. These findings have shown that CM extract inhibited cancer proliferation by increasing ROS levels.
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