HTAPP_Dissociation of human primary colorectal cancer resection specimens into single cell suspension for single cell RNA-seq v1

Abstract

This protocol describes the dissociation of primary human colorectal cancer (CRC) resection specimens into single cell suspensions for single cell RNA-seq applications. It was successfully used for droplet-based single cell RNA-seq of more than 60 patient specimens using the 10x Genomics platform, including 20 HTAPP CRC cohort samples. It can also be used upstream of other single cell RNA-seq approaches such as plate-based SS2 single cell RNA-seq protocols.