Effect of TRAF1 on Phenotypic Changes Of Kupffer Cells In Liver Transplantation Ischemic-Reperfusion
Open Access
- 2 September 2021
- journal article
- research article
- Published by Bentham Science Publishers Ltd. in Current Pharmaceutical Biotechnology
- Vol. 22 (12), 1602-1611
- https://doi.org/10.2174/1389201022666210111113900
Abstract
Objective: The purpose of this study was to explore the effect of TRAF1 on phenotypic changes of KCs in I/R in liver transplantation. Methods: SD rats were randomly divided into sham group and liver transplantation I/R group. KCs were extracted from rat livers in each group. KCs were transfected by lentivirus of si-TRAF1 or si-HOIP, and induced by lipopolysaccharide (LPS). Flow cytometry was used to detect cell apoptosis. Western blot and RT-PCR were used to detect the protein and mRNA expression levels. Results: Compared with sham group, the expression levels of TRAF1, TNF-α and IL-1β were significantly increased in I/R group (P<0.05). In addition, compared with control group, the expression levels of NF-κB (P65 and p-P65) and M1 phenotype (TNF-α and IL-1β) were notably increased in si-TRAF1 and si-HOIP group (all P<0.05). Furthermore, the levels of linear ubiquitin complex (LUBAC) were markedly increased in LPS-induced KCs in comparison with control group (P<0.05). Moreover, compared with control group, the expression levels of P65, p-P65, TNF-α and IL-1β were notably decreased in si-TRAF1 and si-HOIP group (P<0.05). The expression levels of P65, p-P65, TNF-α and IL-1β were significantly increased in si-TRAF1 and si-HOIP group when compared to control group (P<0.05). Conclusion: TRAF1 was an important negative regulator of liver transplantation I/R by inhibiting the activation of NF-κB in KCs and preventing its M1 phenotype transformation.Keywords
This publication has 16 references indexed in Scilit:
- The signaling adaptor TRAF1 negatively regulates Toll-like receptor signaling and this underlies its role in rheumatic diseaseNature Immunology, 2016
- Chronic liver injury in mice promotes impairment of skin barrier functionviatumor necrosis factor-alphaCutaneous and Ocular Toxicology, 2015
- TRAF1 is a critical regulator of cerebral ischaemia–reperfusion injury and neuronal deathNature Communications, 2013
- Opposing Roles for TRAF1 in the Alternative versus Classical NF-κB Pathway in T CellsJournal of Biological Chemistry, 2012
- Linear ubiquitination prevents inflammation and regulates immune signallingNature, 2011
- SHARPIN forms a linear ubiquitin ligase complex regulating NF-κB activity and apoptosisNature, 2011
- A better way to do small-for-size liver transplantation in ratsFrontiers of Medicine, 2011
- NF‐κB signalingWires Systems Biology and Medicine, 2009
- Kupffer cell-dependent TNF-α signaling mediates injury in the arterialized small-for-size liver transplantation in the mouseProceedings of the National Academy of Sciences of the United States of America, 2006
- Kupffer cells: Another player in liver tolerance inductionLiver Transplantation, 2003