Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars
Open Access
- 27 May 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Plant Biology
- Vol. 20 (1), 1-12
- https://doi.org/10.1186/s12870-020-02450-z
Abstract
Low temperature is a major factor influencing the growth and development of Chinese jujube (Ziziphus jujuba Mill.) in cold winter and spring. Little is known about the molecular mechanisms enabling jujube to cope with different freezing stress conditions. To elucidate the freezing-related molecular mechanism, we conducted comparative transcriptome analysis between ‘Dongzao’ (low freezing tolerance cultivar) and ‘Jinsixiaozao’ (high freezing tolerance cultivar) using RNA-Seq. More than 20,000 genes were detected at chilling (4 °C) and freezing (− 10 °C, − 20 °C, − 30 °C and − 40 °C) stress between the two cultivars. The numbers of differentially expressed genes (DEGs) between the two cultivars were 1831, 2030, 1993, 1845 and 2137 under the five treatments. Functional enrichment analysis suggested that the metabolic pathway, response to stimulus and catalytic activity were significantly enriched under stronger freezing stress. Among the DEGs, nine participated in the Ca2+ signal pathway, thirty-two were identified to participate in sucrose metabolism, and others were identified to participate in the regulation of ROS, plant hormones and antifreeze proteins. In addition, important transcription factors (WRKY, AP2/ERF, NAC and bZIP) participating in freezing stress were activated under different degrees of freezing stress. Our research first provides a more comprehensive understanding of DEGs involved in freezing stress at the transcriptome level in two Z. jujuba cultivars with different freezing tolerances. These results may help to elucidate the molecular mechanism of freezing tolerance in jujube and also provides new insights and candidate genes for genetically enhancing freezing stress tolerance.Keywords
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Funding Information
- National Key Research and Development Program of China (2018YFD1000607)
- National Natural Science Foundation of China (31372019)
- Beijing Forestry University (2018BLRD)
This publication has 72 references indexed in Scilit:
- Physio-biochemical and proteome analysis of chickpea in early phases of cold stressJournal of Plant Physiology, 2013
- Alternative Splicing Mediates Responses of the Arabidopsis Circadian Clock to Temperature ChangesTHE PLANT CELL ONLINE, 2012
- A Calcium/Calmodulin-regulated Member of the Receptor-like Kinase Family Confers Cold Tolerance in PlantsPublished by Elsevier BV ,2010
- TopHat: discovering splice junctions with RNA-SeqBioinformatics, 2009
- A Battery of Transcription Factors Involved in the Regulation of Secondary Cell Wall Biosynthesis in ArabidopsisTHE PLANT CELL ONLINE, 2008
- Arabidopsis MAPKs: a complex signalling network involved in multiple biological processesBiochemical Journal, 2008
- Comparative Genomic Sequence and Expression Analyses ofMedicago truncatulaand Alfalfa Subspeciesfalcata COLD-ACCLIMATION-SPECIFICGenesPlant Physiology, 2008
- Cloning and Expression Analysis of a UDP-Galactose/Glucose Pyrophosphorylase from Melon Fruit Provides Evidence for the Major Metabolic Pathway of Galactose Metabolism in Raffinose Oligosaccharide Metabolizing PlantsPlant Physiology, 2006
- The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1Proceedings of the National Academy of Sciences of the United States of America, 2006
- Dual action of the active oxygen species during plant stress responsesCellular and Molecular Life Sciences, 2000