The impact of using culture media containing granulocyte-macrophage colony-stimulating factor on live birth rates in patients with a history of embryonic developmental arrest in previous in vitro fertilization cycles
Open Access
- 1 September 2021
- journal article
- research article
- Published by Galenos Yayinevi in Journal of the Turkish-German Gynecological Association
- Vol. 22 (3), 181-186
- https://doi.org/10.4274/jtgga.galenos.2021.2020.0168
Abstract
Objective: To investigate the effect of using culture media containing granulocyte-macrophage colony-stimulating factor (GM-CSF) on embryological data and reproductive outcomes in patients with early embryonic developmental arrest. Material and Methods: Retrospective case-control study. A total of 39 patients, whose embryos were incubated with culture media containing GM-CSF due to embryonic developmental arrest in two previous in vitro fertilization (IVF) cycles in-between January 2016 and November 2017 at Hacettepe University IVF Center, were enrolled. Control group was generated among patients with first IVF attempts due to tubal factor in the same time period. All embryos in the control group were incubated with single step culture medium (without GM-CSF). For the control group selection, matching was done 1:2 ratio considering female age, body mass index, number of M-II oocyte retrieved, and number of embryo transferred (n=80). Results: Demographic features and embryological data were comparable between two groups. Number of fertilized oocytes (2-pronuclear) was 3.7 +/- 2.0 in GM-CSF group and 3.9 +/- 2.5 in the control (p=0.576). Overall, number of embryos transferred (1.3 +/- 0.5 vs 1.3 +/- 0.5, respectively) and blastocyst transfer rate (67.6% vs 59.2%, respectively; p=0.401) were similar. For the reproductive outcomes, implantation rate (32.3% vs 33.1%, respectively; p=0.937), clinical pregnancy rate (33.3% vs 32.5%, respectively; p=0.770), and live birth rate (25.2% vs 26.2%, respectively; p=0.943) were similar. Conclusion: Using GM-CSF-containing culture media in patients with two previous failed IVF attempts due to embryonic developmental arrest might rectify embryological data and reproductive outcomes. To make solid conclusion further randomized controlled trials are warranted.Keywords
This publication has 31 references indexed in Scilit:
- A randomized clinical trial to evaluate the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) in embryo culture medium for in vitro fertilizationFertility and Sterility, 2013
- Granulocyte Macrophage Colony Stimulating Factor Supplementation in Culture Media for Subfertile Women Undergoing Assisted Reproduction Technologies: A Systematic ReviewInternational Journal of Endocrinology, 2013
- Use of granulocyte colony-stimulating factor for the treatment of unexplained recurrent miscarriage: a randomised controlled trialHuman Reproduction, 2009
- Upregulation of Chemokine and Cytokine Production during PregnancyGynecologic and Obstetric Investigation, 2008
- Permanent embryo arrest: molecular and cellular conceptsMolecular Human Reproduction, 2008
- Telomere lengthening early in developmentNature, 2007
- Apoptosis in the human embryoReviews of Reproduction, 1999
- Isolation of a Receptor Tyrosine Kinase (DTK) from Embryonic Stem Cells: Structure, Genetic Mapping and Analysis of ExpressionGrowth Factors, 1994
- Uterine Epithelial Cells Synthesize Granulocyte-Macrophage Colony-Stimulating Factor and Interleukin-6 in Pregnant and Nonpregnant Mice1Biology of Reproduction, 1992
- Human gene expression first occurs between the four- and eight-cell stages of preimplantation developmentNature, 1988