Pig-to-Macaque Islet Xenotransplantation
- 31 January 2020
- book chapter
- protocol
- Published by Springer Science and Business Media LLC
- Vol. 2110, 289-314
- https://doi.org/10.1007/978-1-0716-0255-3_19
Abstract
The advancement toward a clinical application for porcine islets to cure diabetes in humans must include reproducible long-term successes in non-human primate (NHP) models. Many dedicated researchers around the world are continuing to work toward this goal. In this chapter, we describe procedures for islet isolation of pancreatic islets from adult and neonatal/fetal pigs. We further include procedures for the induction of diabetes in non-human primates and subsequent insulin therapy, islet transplantation, immunosuppression, and also the daily maintenance of xenotransplanted NHPs. The procedures that we outline in this chapter are ones that we have successfully utilized in pig-to-NHP islet transplantation models. However, where appropriate, alternative methods will also be identified.Keywords
This publication has 24 references indexed in Scilit:
- Improved Health-Related Quality of Life in a Phase 3 Islet Transplantation Trial in Type 1 Diabetes Complicated by Severe HypoglycemiaDiabetes Care, 2018
- A Comparative Analysis of the Safety, Efficacy, and Cost of Islet Versus Pancreas Transplantation in Nonuremic Patients With Type 1 DiabetesAmerican Journal of Transplantation, 2015
- Islet xenotransplantation: what is the optimal age of the islet‐source pig?Xenotransplantation, 2014
- Clinical Islet XenotransplantationDiabetes, 2012
- Reduced Progression of Diabetic Microvascular Complications With Islet Cell Transplantation Compared With Intensive Medical TherapyTransplantation, 2011
- The Clinical Impact of Islet TransplantationAmerican Journal of Transplantation, 2008
- What strain of pig should be used?Xenotransplantation, 2008
- Five-Year Follow-Up After Clinical Islet TransplantationDiabetes, 2005
- Islet Transplantation in Seven Patients with Type 1 Diabetes Mellitus Using a Glucocorticoid-Free Immunosuppressive RegimenThe New England Journal of Medicine, 2000
- Pancreas transplantation: a reviewTransplantation Proceedings, 1998