Parallel multiphoton excited fabrication of tissue engineering scaffolds using a diffractive optical element
- 21 January 2020
- journal article
- research article
- Published by Optica Publishing Group in Optics Express
- Vol. 28 (3), 2744-2757
- https://doi.org/10.1364/oe.381362
Abstract
Multiphoton excited photochemistry is a powerful technique for freeform nano/microfabrication. However, the construction of large and complex structures using single point scanning is slow, where this is a significant limitation for biological investigations. We demonstrate increased throughput via parallel fabrication using a diffractive optical element. To implement an approach with large field of view and near-theoretical resolution, a scan lens was designed that is optimized for using low-magnification high NA objective lenses. We demonstrate that with this approach it is possible to synthesize large scaffolds at speeds several times faster than by single point scanning.Funding Information
- National Cancer Institute (R01 CA206561-01)
- National Heart, Lung, and Blood Institute (1R21HL126190-01A1, R01 HL131017-01A)
- University of Wisconsin-Madison
This publication has 67 references indexed in Scilit:
- Cell-laden microengineered gelatin methacrylate hydrogelsBiomaterials, 2010
- Biomimetic hydrogels with pro-angiogenic propertiesBiomaterials, 2010
- Adhesion and migration of ovarian cancer cells on crosslinked laminin fibers nanofabricated by multiphoton excited photochemistry.Integrative Biology, 2009
- Multiphoton fabrication of chemically responsive protein hydrogels for microactuationProceedings of the National Academy of Sciences of the United States of America, 2008
- Parallel photopolymerisation with complex light patterns generated by diffractive optical elementsOptics Express, 2007
- Improving spatial resolution of two-photon microfabrication by using photoinitiator with high initiating efficiencyApplied Physics Letters, 2007
- Multiphoton excited fabricated nano and micro patterned extracellular matrix proteins direct cellular morphologyJournal of Biomedical Materials Research Part A, 2006
- Properties of crosslinked protein matrices for tissue engineering applications synthesized by multiphoton excitationJournal of Biomedical Materials Research Part A, 2004
- A Novel Water-Soluble Photoinitiator for the Acrylic Photopolymerization Type Resist SystemChemistry of Materials, 1998
- Photoinitiators for UV and visible curing of coatings: Mechanisms and propertiesJournal of Photochemistry and Photobiology A: Chemistry, 1996