Electroacupuncture Relieves Pain and Attenuates Inflammation Progression Through Inducing IL-10 Production in CFA-Induced Mice
- 20 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Inflammation
- Vol. 43 (4), 1233-1245
- https://doi.org/10.1007/s10753-020-01203-2
Abstract
The therapeutic effect of electroacupuncture (EA) on inflammatory pain has been well recognized clinically, but the mechanism is unclear. Interleukin-10 (IL-10), which is produced by regulatory T (Treg) cell, is a key anti-inflammatory cytokine for relieving inflammatory pain. Therefore, the aim of this study is to investigate whether EA could inhibit CFA-induced pain and attenuate inflammation progression by regulating the activation of immunocyte and inducing the expression of IL-10. In this study, mice were treated with EA (2/100 Hz, 2 mA) for five consecutive days after 1 day of CFA injection. The behavioral tests were measured and analyzed after the daily EA treatment; then, hind paw, spinal cord, and spleen tissues were prepared for assessment. The results showed that EA treatment significantly increased the mechanical threshold and thermal latency after CFA injection and boosted the expression of IL-10 in paw and spinal cord tissues. EA treatment promoted Treg cells; suppressed macrophage and neutrophils cells; reduced the expression of IL-1β, NLRP3, and TNF-α; and ultimately relieved inflammatory pain. The findings suggested that the analgesic and anti-inflammatory effect of EA treatment could be partially associated with suppression of pro-inflammatory cytokines mediated by induction of IL-10.Keywords
Funding Information
- National Natural Science Foundation of China (81774210, 81574062)
- Six Talent Peaks Project in Jiangsu Province (YY-033)
- QingLan Project of Jiangsu Province
- 333 High-level Talents Cultivating Project of Jiangsu Province (2016)
- A Project Funded by the Nursing Priority Academic Program Development of Jiangsu Higher Education Institutions (2019YSHL046)
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