Identification and Characterization of Two Novel IS CR1 -Associated Genes, dfrA42 and dfrA43 , Encoding Trimethoprim-Resistant Dihydrofolate Reductases
- 19 April 2021
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 65 (5)
- https://doi.org/10.1128/aac.02010-20
Abstract
Two novel ISCR1-associated dfr genes, dfrA42 and dfrA43, were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli. These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.Funding Information
- National Natural Science Foundation of China (31770042, 31770043)
- National Key Research and Development Program of China (2017YFD0400301)
- Fundamental Research Fund of Shandong University (2018JC013, 2018JC027)
- State Key Laboratory of Microbial Technology Open Project Funds, Shandong University (M2019-04)
- Key R&D Program of Shandong Province (2020CXGC011305)
- Shandong Provincial Natural Science Foundation, China (ZR2020MH308)
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