Inhibition of aflatoxin biosynthesis in Aspergillus flavus by phenolic compounds extracted of Piper betle L
Open Access
- 1 December 2013
- journal article
- Vol. 5 (4), 428-433
Abstract
Food contamination by aflatoxins is an important food safety concern for agricultural products. In order to identify and develop novel antifungal agents, several plant extracts and isolated compounds have been evaluated for their bioactivities. Anti-infectious activity of Piper betle used in traditional medicine of Malaysia has been reported previously. Crude methanol extract from P. betel powdered leaves was partitioned between chloroform and water. The fractions were tested against A. flavus UPMC 89, a strong aflatoxin producing strain. Inhibition of mycelial growth and aflatoxin biosynthesis were tested by disk diffusion and macrodillution techniques, respectively. The presence of aflatoxin was determined by thin-layer chromatography (TLC) and fluorescence spectroscopy techniques using AFB1 standard. The chloroform soluble compounds were identified using HPLC-Tandem mass spectrometry technique. The results, evaluated by measuring the mycelial growth and quantification of aflatoxin B1(AFLB1) production in broth medium revealed that chloroform soluble compounds extract from P. betle dried leaves was able to block the aflatoxin biosynthesis pathway at concentration of 500μg/ml without a significant effect on mycelium growth. In analyzing of this effective fractions using HPLC-MS2 with ESI ionization technique, 11 phenolic compounds were identified. The results showed that the certain phenolic compounds are able to decline the aflatoxin production in A. flavus with no significant effect on the fungus mycelia growth. The result also suggested P. betle could be used as potential antitoxin product.Keywords
This publication has 16 references indexed in Scilit:
- The lipid language of plant–fungal interactionsFungal Genetics and Biology, 2011
- In vitro antifungal activity of hydroxychavicol isolated from Piper betle LAnnals of Clinical Microbiology and Antimicrobials, 2010
- Characterization of ESBL (SHV-12) producing clinical isolate of Enterobacter aerogenes from a tertiary care hospital in NigeriaAnnals of Clinical Microbiology and Antimicrobials, 2010
- Antimicrobial activity of the methanolic extract, fractions and four flavonoids from the twigs of Dorstenia angusticornis Engl. (Moraceae)Journal of Ethnopharmacology, 2007
- Medicinal plants: Traditions of yesterday and drugs of tomorrowMolecular Aspects of Medicine, 2006
- Antibacterial properties of essential oils from Thai medicinal plantsFitoterapia, 2005
- Inhibition of growth and mycotoxin production of Aspergillus flavus and Aspergillus parasiticus by extracts of Agave speciesInternational Journal of Food Microbiology, 2005
- New Additive for Culture Media for Rapid Identification of Aflatoxin-Producing Aspergillus StrainsApplied and Environmental Microbiology, 2001
- Laboratory evaluation of chemical control of aflatoxin production in unshelled peanuts(Arachis hypogaeaL.)Food Additives & Contaminants, 1995
- Acetoxychavicol Acetate, an Antifungal Component ofAlpinia galanga1Planta Medica, 1985