Effects of Zinc Oxide Nanoparticles on Physiological and Anatomical Indices in Spring Barley Tissues
Open Access
- 29 June 2021
- journal article
- research article
- Published by MDPI AG in Nanomaterials
- Vol. 11 (7), 1722
- https://doi.org/10.3390/nano11071722
Abstract
The aim of the present work was to investigate the toxic effects of zinc oxide nanoparticles (ZnO NPs, particle size < 50 nm) on the physiological and anatomical indices of spring barley (Hordeum sativum L.). The results show that ZnO NPs inhibited H. sativum growth by affecting the chlorophyll fluorescence emissions and causing deformations of the stomatal and trichome morphology, alterations to the cellular organizations, including irregularities of the chloroplasts, and disruptions to the grana and thylakoid organizations. There was a lower number of chloroplasts per cell observed in the H. sativum leaf cells treated with ZnO NPs as compared to the non-treated plants. Cytomorphometric quantification revealed that ZnO NPs decreased the size of the chloroplast by 1.5 and 4 times in 300 and 2000 mg/L ZnO NP-treated plants, respectively. The elemental analysis showed higher Zn accumulation in the treated leaf tissues (3.8 and 10.18-fold with 300 and 2000 mg/L ZnO NPs, respectively) than the untreated. High contents of Zn were observed in several spots in ZnO NP-treated leaf tissues using X-ray fluorescence. Deviations in the anatomical indices were significantly correlated with physiological observations. The accumulation of Zn content in plant tissues that originated from ZnO NPs was shown to cause damage to the structural organization of the photosynthetic apparatus and reduced the photosynthetic activities.This publication has 64 references indexed in Scilit:
- Plant nanobionics approach to augment photosynthesis and biochemical sensingNature Materials, 2014
- Effect of soluble copper released from copper oxide nanoparticles solubilisation on growth and photosynthetic processes ofLemna gibbaLNanotoxicology, 2013
- ZnO Nanoparticle Biosynthesis and Its Effect on Phosphorous-Mobilizing Enzyme Secretion and Gum Contents in Clusterbean (Cyamopsis tetragonoloba L.)Agricultural Research, 2013
- Interaction of Nanoparticles with Edible Plants and Their Possible Implications in the Food ChainJournal of Agricultural and Food Chemistry, 2011
- Evidence of the Differential Biotransformation and Genotoxicity of ZnO and CeO2 Nanoparticles on Soybean (Glycine max) PlantsEnvironmental Science & Technology, 2010
- Colloidal suspensions of clay or titanium dioxide nanoparticles can inhibit leaf growth and transpiration via physical effects on root water transportPlant, Cell & Environment, 2009
- Chlorophyll fluorescence imaging of photosynthetic activity in sun and shade leaves of treesPhotosynthesis Research, 2007
- Plastoglobules Are Lipoprotein Subcompartments of the Chloroplast That Are Permanently Coupled to Thylakoid Membranes and Contain Biosynthetic EnzymesTHE PLANT CELL ONLINE, 2006
- Changes of chloroplast ultrastructure and total chlorophyll concentration in cabbage leaves caused by excess of organic Ni(II) complexesEnvironmental and Experimental Botany, 2002
- Differences in sensitivity of the photosynthetic apparatus in Cd-stressed runner bean plants in relation to their agePlant Science, 1997