Retinal capillary oximetry with visible light optical coherence tomography
- 26 May 2020
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 117 (21), 11658-11666
- https://doi.org/10.1073/pnas.1918546117
Abstract
Assessing oxygen saturation (sO(2)) remains challenging but is nonetheless necessary for understanding retinal metabolism. We and others previously achieved oximetry on major retinal vessels and measured the total retinal oxygen metabolic rate in rats using visible-light optical coherence tomography. Here we extend oximetry measurements to capillaries and investigate all three retinal vascular plexuses by amplifying and extracting the spectroscopic signal from each capillary segment under the guidance of optical coherence tomography (OCT) angiography. Using this approach, we measured capillary sO(2) in the retinal circulation in rats, demonstrated reproducibility of the results, validated the measurements in superficial capillaries with known perfusion pathways, and determined sO(2) responses to hypoxia and hyperoxia in the different retinal capillary beds. OCT capillary oximetry has the potential to provide new insights into the retinal circulation in the normal eye as well as in retinal vascular diseases.Funding Information
- HHS | NIH | National Eye Institute (R01 EY027833)
- HHS | NIH | National Eye Institute (R01 EY024544)
- HHS | NIH | National Eye Institute (R01 EY010145)
- HHS | NIH | National Eye Institute (P30 EY010572)
- Research to Prevent Blindness (William & Mary Greve Special Scholar Award)
This publication has 58 references indexed in Scilit:
- Oxygen consumption and distribution in the Long-Evans rat retinaExperimental Eye Research, 2012
- Split-spectrum amplitude-decorrelation angiography with optical coherence tomographyOptics Express, 2012
- Oxygen modulation of neurovascular coupling in the retinaProceedings of the National Academy of Sciences of the United States of America, 2011
- Two-photon high-resolution measurement of partial pressure of oxygen in cerebral vasculature and tissueNature Methods, 2010
- Retinal vessel oximetry-calibration, compensation for vessel diameter and fundus pigmentation, and reproducibilityJournal of Biomedical Optics, 2008
- Retinal angiogenesis in development and diseaseNature, 2005
- Erythrocyte-associated transients in Po2revealed in capillaries of rat mesenteryAmerican Journal of Physiology-Heart and Circulatory Physiology, 2005
- Molecular Stability and Function of Mouse HemoglobinsZoological Science, 1998
- Scanning electron-microscopic study of corrosion casts on retinal and choroidal angioarchitecture in man and animalsProgress in Retinal and Eye Research, 1994
- A note on two problems in connexion with graphsNumerische Mathematik, 1959