Our fifth contribution to the checklist of Algerian fungi is dedicated to Dothideomycetes which comprise a highly diverse range of fungi characterized mainly by asci with double two wall layers (bitunicate asci) and often with fissitunicate dehiscence. By screening all available bibliographic sources and herbaria (fungaria) catalogues it was possible to delineate 562 species belonging to 59 families and 176 genera that occurs in Algeria. Additionally, a list of 10 doubtful records unsupported by literature and/or herbarium (fungarium) material is provided. This checklist is the first comprehensive species list fully dedicated to Algerian Dothideomycetes. Therefore, it should be mentioned here that, although the present study will add some new data to our information concerning Fungi in Algeria, this checklist must be considered as a provisional one always waiting for continuous supplementation.