Women with chronic follicular gastritis positive for Helicobacter pylori express lower levels of GKN1
- 21 February 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Gastric Cancer
- Vol. 23 (4), 754-759
- https://doi.org/10.1007/s10120-020-01049-5
Abstract
In women, serum levels of CTSB, GKN2, LIPF, LIPFG, AZGP1, TOP2A and PGA4 are proposed as predictive markers of gastric cancer. It is unknown whether GKN1 expression varies with the sex of patients with chronic gastritis or gastric cancer. We studied 36 patients with histopathological diagnosis of chronic gastritis from the state of Guerrero, Mexico. PCR was performed for H. pylori detection and GKN1 expression was determined by RT-qPCR and western blot. GKN1 mRNA expression was significantly lower in patients with chronic follicular gastritis than in those with chronic chemical gastritis (p = 0.00071). The mRNA and protein level of expression of GKN1 were significantly lower in women with chronic follicular gastritis than in men with the same condition (p = 0.0279 and p = 0.0014, respectively); the lowest levels of GKN1 were detected in women with H. pylori-positive follicular gastritis (p = 0.0175 and p = 0.0111, respectively). Through a bioinformatic analysis, estrogen response elements were identified in the GKN1 promoter.Keywords
Funding Information
- Consejo Nacional de Ciencia y Tecnología (CB-2015-01)
- Secretaría de Educación Pública (PFCE-SEP-2017 and 2018)
- Consejo Nacional de Ciencia y Tecnología (296364, 777741)
This publication has 24 references indexed in Scilit:
- The Eukaryotic Promoter Database: expansion of EPDnew and new promoter analysis toolsNucleic Acids Research, 2014
- MicroRNA and signaling pathways in gastric cancerCancer Gene Therapy, 2014
- The Role of Gastrokine 1 in Gastric CancerJournal of Gastric Cancer, 2014
- Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosisJournal of Experimental & Clinical Cancer Research, 2012
- Inactivation of the Gastrokine 1 gene in gastric adenomas and carcinomasThe Journal of Pathology, 2010
- An integrated transcriptomic and computational analysis for biomarker identification in gastric cancerNucleic Acids Research, 2010
- Oestrogen and the enigmatic male predominance of gastric cancerEuropean Journal of Cancer, 2008
- Decreased Expression of Gastrokine 1 and the Trefoil Factor Interacting Protein TFIZ1/GKN2 in Gastric Cancer: Influence of Tumor Histology and Relationship to PrognosisClinical Cancer Research, 2008
- Gastrokine 1 expression in patients with and without Helicobacter pylori infectionDigestive and Liver Disease, 2007
- Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell lineNucleic Acids Research, 1982