The Polarity of an Amino Acid at Position 1891 of Severe Fever with Thrombocytopenia Syndrome Virus L Protein Is Critical for the Polymerase Activity
Open Access
- 27 December 2020
- Vol. 13 (1), 33
- https://doi.org/10.3390/v13010033
Abstract
Severe fever with thrombocytopenia syndrome virus subclone B7 shows strong plaque formation and cytopathic effect induction compared with other subclones and the parental strain YG1. Compared to YG1 and the other subclones, only B7 possesses a single substitution in the L protein at the amino acid position 1891, in which N is changed to K (N1891K). In this study, we evaluate the effects of this mutation on L protein activity via a cell-based minigenome assay. Substitutions of N with basic amino acids (K or R) enhanced polymerase activity, while substitutions with an acidic amino acid (E) decreased this activity. Mutation to other neutral amino acids showed no significant effect on activity. These results suggest that the characteristic of the amino acid at position 1891 of the L protein are critical for its function, especially with respect to the charge status. Our data indicate that this C-terminal domain of the L protein may be crucial to its functions in genome transcription and viral replication.Keywords
This publication has 34 references indexed in Scilit:
- Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome VirusJournal of Virology, 2015
- Amino Acid Substitutions in Polymerase Basic Protein 2 Gene Contribute to the Pathogenicity of the Novel A/H7N9 Influenza Virus in Mammalian HostsJournal of Virology, 2014
- The First Identification and Retrospective Study of Severe Fever With Thrombocytopenia Syndrome in JapanThe Journal of Infectious Diseases, 2013
- Severe Fever with Thrombocytopenia Syndrome, South Korea, 2012Emerging Infectious Diseases, 2013
- Epidemiologic Features of Severe Fever With Thrombocytopenia Syndrome in China, 2011-2012Clinical Infectious Diseases, 2013
- A Family Cluster of Infections by a Newly Recognized Bunyavirus in Eastern China, 2007: Further Evidence of Person-to-Person TransmissionClinical Infectious Diseases, 2011
- Fever with Thrombocytopenia Associated with a Novel Bunyavirus in ChinaThe New England Journal of Medicine, 2011
- Bunyaviridae RNA Polymerases (L-Protein) Have an N-Terminal, Influenza-Like Endonuclease Domain, Essential for Viral Cap-Dependent TranscriptionPLoS Pathogens, 2010
- The N-Terminal Domain of the Arenavirus L Protein Is an RNA Endonuclease Essential in mRNA TranscriptionPLoS Pathogens, 2010
- Molecular Basis for High Virulence of Hong Kong H5N1 Influenza A VirusesScience, 2001