Culture-based Methods for Studying the Bacterial Root Microbiome of Wheat
- 8 November 2020
- book chapter
- protocol
- Published by Springer Science and Business Media LLC
- Vol. 2232, 53-60
- https://doi.org/10.1007/978-1-0716-1040-4_6
Abstract
Beneficial plant-microbe interactions are important and desirable for sustainable intensification of agriculture. Here, we describe methods to isolate microbes from the roots of field-grown wheat plants. This includes the rhizosphere and rhizoplane soil, as well as the root endosphere. We also describe a method to test for endosphere competence of putative endophytes.Keywords
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