Overexpression of the Transcription Factor AtLEC1 Significantly Improved the Lipid Content of Chlorella ellipsoidea
Open Access
- 17 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Bioengineering and Biotechnology
Abstract
Microalgae are considered to be a highly promising source for the production of biodiesel. However, the regulatory mechanism governing lipid biosynthesis has not been fully elucidated to date, and the improvement of lipid accumulation in microalgae is essential for the effective production of biodiesel. In this study, LEAFY COTYLEDON1 (LEC1) from Arabidopsis thaliana, a transcription factor (TF) that affects lipid content, was transferred into Chlorella ellipsoidea. Compared with wild-type (WT) strains, the total fatty acid content and total lipid content of AtLEC1 transgenic strains were significantly increased by 24.20–32.65 and 22.14–29.91%, respectively, under mixotrophic culture conditions and increased by 24.4–28.87 and 21.69–30.45%, respectively, under autotrophic conditions, while the protein content of the transgenic strains was significantly decreased by 18.23–21.44 and 12.28–18.66%, respectively, under mixotrophic and autotrophic conditions. Fortunately, the lipid and protein content variation did not affect the growth rate and biomass of transgenic strains under the two culture conditions. According to the transcriptomic data, the expression of 924 genes was significantly changed in the transgenic strain (LEC1-1). Of the 924 genes, 360 were upregulated, and 564 were downregulated. Based on qRT-PCR results, the expression profiles of key genes in the lipid synthesis pathway, such as ACCase, GPDH, PDAT1, and DGAT1, were significantly changed. By comparing the differentially expressed genes (DEGs) regulated by AtLEC1 in C. ellipsoidea and Arabidopsis, we observed that approximately 59% (95/160) of the genes related to lipid metabolism were upregulated in AtLEC1 transgenic Chlorella. Our research provides a means of increasing lipid content by introducing exogenous TF and presents a possible mechanism of AtLEC1 regulation of lipid accumulation in C. ellipsoidea.Keywords
This publication has 67 references indexed in Scilit:
- Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed DevelopmentPlant Physiology, 2013
- Enhanced Seed Oil Production in Canola by Conditional Expression of Brassica napus LEAFY COTYLEDON1 and LEC1-LIKE in Developing SeedsPlant Physiology, 2011
- MicroRNAs Regulate the Timing of Embryo Maturation in ArabidopsisPlant Physiology, 2011
- Expression of ZmLEC1 and ZmWRI1 Increases Seed Oil Production in MaizePlant Physiology, 2010
- Identification and functional analysis of the genes encoding 6-desaturase from Ribes nigrumJournal of Experimental Botany, 2010
- SOAP2: an improved ultrafast tool for short read alignmentBioinformatics, 2009
- Assessment of Cr and Ni phytotoxicity from cutlery-washing waste-waters using biomass and chlorophyll production tests on mustard Sinapis alba L. seedlingsEnvironmental Science and Pollution Research, 2009
- LEAFY COTYLEDON1Is a Key Regulator of Fatty Acid Biosynthesis in ArabidopsisPlant Physiology, 2008
- Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advancesThe Plant Journal, 2008
- Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics researchBioinformatics, 2005