Under oil open-channel microfluidics empowered by exclusive liquid repellency
Open Access
- 17 April 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (16), eaay9919
- https://doi.org/10.1126/sciadv.aay9919
Abstract
Recently, the functionality of under oil open microfluidics was expanded from droplet-based operations to include lateral flow in under oil aqueous channels. However, the resolution of the under oil fluidic channels reported so far is still far from comparable with that of closed-channel microfluidics (millimeters versus micrometers). Here, enabled by exclusive liquid repellency and an under oil sweep technique, open microchannels can now be prepared under oil (rather than in air), which shrinks the channel dimensions up to three orders of magnitude compared to previously reported techniques. Spatial trapping of different cellular samples and advanced control of mass transport (i.e., enhanced upper limit of flow rate, steady flow with passive pumping, and reversible fluidic valves) were achieved with open-channel designs. We apply these functional advances to enable dynamic measurements of dispersion from a pathogenic fungal biofilm. The ensemble of added capabilities reshapes the potential application space for open microfluidics.Funding Information
- National Science Foundation (EFRI-1136903-EFRI-MKS)
- National Institutes of Health (R01 EB010039 BRG)
- National Institutes of Health (R01 CA185251)
- National Institutes of Health (R01 CA186134)
- National Institutes of Health (R01 CA181648)
- National Institutes of Health (R21 AI135166)
- American Cancer Society (IRG-15-213-51)
- U.S. Environmental Protection Agency (H-MAP 83573701)
- U.S. Environmental Protection Agency (H-MAP 83573701)
- U.S. Environmental Protection Agency (H-MAP 83573701)
- Career Award at the Scientific Interface from Burroughs Wellcome Fund
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